Construction of a nitrite sensor based on biocatalyst electrode

基于生物催化电极原理，研制了一种亚硝酸根传感器。以血红素蛋白质如细胞色素c为催化剂，电化学还原亚硝酸盐.研究发现，在酸性溶液中，微量细胞色素c的存在能在较低的负电位下有效地还原亚硝酸盐.即使在100 ℃下处理30 min，细胞色素c的催化效果也保持不变.结果表明，该催化剂的催化活性是由于细胞色素c吸附在电极表面所致.利用黄血红素蛋白和醌血红素蛋白详细研究了吸附血红素蛋白的电化学行为.结果表明，利用血红素蛋白、细胞色素c、肌红蛋白和血红蛋白可以构建亚硝酸盐传感器.甲基紫精也被发现是有效的催化剂，以减少亚硝酸盐在电极上的过电位在中性溶液中。

A nitrite sensor was developed on the basis of the principle of biocatalyst electrodes. Heme proteins such as cytochrome c was employed as a catalyst to reduce nitrite electrochemically.1. It was found that the presence of a trace amount of cytochrome c was effective to reduce nitrite at less negative potentials in acidic solutions.2. The catalytic effect of cytochrome c remained unchanged even when treated at lOO ﾟC for 30 min.3. It was revealed that the catalytic activity was due to cytochrome c adsorbed on the surface of electrodes.4. The electrochemical behavior of adsorbed heme-proteins was studied in detail by the use of flavohemo-proteins ans quinohemo proteins.5. It was shown that nitite sensors could be constructed by usisng heme proteins, cytochrome c, myoglobin and hemeglobin.6. Methyl viologen was found to be also effective as a catalyst to reduce nitite at electrodes with a reduced overpotential in neutral solutions.