Construction of a nitrite sensor based on biocatalyst electrode

基于生物催化剂电极的亚硝酸盐传感器的构建

• 批准号: 04660142
• 负责人: IKEDA Tokuji
• 金额: $ 1.28万
• 依托单位: KYOTO UNIVERSITY
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for General Scientific Research (C)
• 财政年份: 1992
• 资助国家: 日本
• 起止时间: 1992 至 1993
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-04660142/
• 关键词: nitrite sensor; biocatalyst electrode; cytochrome c; bioelectrocatalysis; チトクロムC

A nitrite sensor was developed on the basis of the principle of biocatalyst electrodes. Heme proteins such as cytochrome c was employed as a catalyst to reduce nitrite electrochemically.1. It was found that the presence of a trace amount of cytochrome c was effective to reduce nitrite at less negative potentials in acidic solutions.2. The catalytic effect of cytochrome c remained unchanged even when treated at lOO ﾟC for 30 min.3. It was revealed that the catalytic activity was due to cytochrome c adsorbed on the surface of electrodes.4. The electrochemical behavior of adsorbed heme-proteins was studied in detail by the use of flavohemo-proteins ans quinohemo proteins.5. It was shown that nitite sensors could be constructed by usisng heme proteins, cytochrome c, myoglobin and hemeglobin.6. Methyl viologen was found to be also effective as a catalyst to reduce nitite at electrodes with a reduced overpotential in neutral solutions.

基于生物催化电极原理，研制了一种亚硝酸根传感器。以血红素蛋白质如细胞色素c为催化剂，电化学还原亚硝酸盐.研究发现，在酸性溶液中，微量细胞色素c的存在能在较低的负电位下有效地还原亚硝酸盐.即使在100 ℃下处理30 min，细胞色素c的催化效果也保持不变.结果表明，该催化剂的催化活性是由于细胞色素c吸附在电极表面所致.利用黄血红素蛋白和醌血红素蛋白对血红素蛋白的电化学行为进行了详细的研究.结果表明，利用血红素蛋白、细胞色素c、肌红蛋白和血红蛋白可以构建亚硝酸盐传感器.甲基紫精也被发现是有效的催化剂，以减少亚硝酸盐在电极上的过电位在中性溶液中。

项目成果

池田篤治: "Electrocatalytic activity of cytochrome C for the reduction of nitric oxide" Electro-analysis. 6(in press). (1994)

Atsuji Ikeda：“细胞色素 C 还原一氧化氮的电催化活性”电分析 6（出版中）。

Tokuji Ikeda: "Enzyme-catalysed electrochemical oxidation of D-gluconate at electrode coated with D-DLDH, a membranebound flavohemoprotein" J.Electroanal.Chem.352. 267-278 (1993)

Tokuji Ikeda：“在涂有 D-DLDH（一种膜结合黄素血蛋白）的电极上 D-葡萄糖酸的酶催化电化学氧化”J.Electroanal.Chem.352。

池田篤治: "Bioelectrocatalysis at electrodes coated with alcohol dehydlogenase,a quinokemoprotein with hemec serving as a built-in mediater" J.Electroanal.Chem.361. 221-228 (1993)

Atsuji Ikeda：“涂有乙醇脱氢酶的电极上的生物电催化，一种以血红素作为内置介质的醌蛋白”J.Electroanal.Chem.361 (1993)。

池田篤治: "Bioelectrocatalysis at electrodes coated with alcohol dehydrogenase,a quinohemoprotein with heme serving as a built-in mediator." J.Electroanal.Chem.361. 221-228 (1993)

Atsuji Ikeda：“涂有乙醇脱氢酶的电极的生物电催化，一种以血红素作为内置介质的奎宁血红素蛋白。”J.Electroanal.Chem.361 (1993)。

池田篤治: "Enzyme-catalyzed electrochemical oxidation of D-gluconate at electrode coated with D-GLDH,a membrane-bound flavohemo protein" J.Electroanal.Chem.352. 267-278 (1993)

Atsuji Ikeda：“D-葡萄糖酸在涂有 D-GLDH（一种膜结合黄素血蛋白）的电极上的酶催化电化学氧化”J.Electroanal.Chem.352 (1993)。