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Structural Analysis of Sulfated Polysaccharide with Anti-AIDS Virus.

抗艾滋病病毒硫酸多糖的结构分析。

基本信息

批准号：
04660226
负责人：
OKUTANI Koichi
金额：
$ 1.34万
依托单位：
Kagawa University
依托单位国家：
日本
项目类别：
Grant-in-Aid for General Scientific Research (C)
财政年份：
1992
资助国家：
日本
起止时间：
1992 至 1993
项目状态：
已结题

项目摘要

A marine strain of Pseudomonas (HA-318) produces a polysaccharide with a rate component, fucosamine. The polysaccharide was extracted with 1% phenol solution and purified by addition of ethanool followed by precipitation with Cetevlon and DEAE-cellulose eolumn chromatography. This polysaccharide preparation, which was homogeneous in both ultracentrifugal and electrophoretic analyzes, was composed of galacturonic acid, galactose, fucosamine, and pyruvate in the molar rations of 3 : 1 : 1 : 1, together with N-acetyl groups.Sulfated derivatives of the polysaccharide prepared by chlorosulfonic acid treatment of a fucosamine-containing polysaccharide from a marine Pseudomonas showed strong anti-viral activity ageinst herpes simplex type 1 (HSV-1). This sulfated polysaccharide inhibited the cytopathic effect of HSV-1 at 0.72 ug/ml. No cytotoxic effects to Vero cells were detected with concentrations of sulfated polysaccharide up to 1,000 ug/ml.Sulfated derivatives was fractionated by chromatography on a DEAE-celulose column. The fraction eluted with 0.6M NaCl showed the anti-HIV-1 activity (IC_<50>=0.69 ug/ml) which was higher than that of dextran sulfate(1.5 ug/ml). No cytotoxicity of this sample for MT-4 cells was noted at concentration up to 100 ug/ml. It should be noted that this showed an anti-HIV-1 effect at a lower degree of sulfation (S=5.6%, W/W) than dextran sulfate (S >17%, W/W).

海洋假单胞菌菌株（HA-318）产生具有速率组分岩藻糖胺的多糖。用1%苯酚溶液提取多糖，加入乙醇，然后用十六烷基硫酸钠和DEAE-纤维素柱层析进行纯化。该多糖制剂在超电泳和电泳分析中均为均一的，由半乳糖醛酸、半乳糖、岩藻糖胺和丙酮酸以3：1：1：1的摩尔比组成。1,通过氯磺酸处理海洋假单胞菌的含岩藻糖胺的多糖制备的多糖的硫酸化衍生物显示出强的抗-1型单纯疱疹病毒（HSV-1）。该硫酸多糖在0.72 μ g/ml时抑制HSV-1的细胞病变效应。硫酸化多糖浓度高达1，000 μ g/ml时，未检测到对Vero细胞的细胞毒性作用。硫酸化衍生物通过色谱法在DEAE-纤维素柱上分级分离。用0.6M NaCl洗脱的组分显示出抗HIV-1活性（IC_<50>=0.69 μ g/ml），高于硫酸葡聚糖（1.5 μ g/ml）。在高达100 μ g/ml的浓度下，未观察到该样品对MT-4细胞的细胞毒性。应该注意的是，这显示出在比硫酸葡聚糖（S > 17%，W/W）更低的硫酸化程度（S= 5.6%，W/W）下的抗HIV-1效果。