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Structural Aanalyzes and the Immunostimulant Activity of the Anti-AIDS Virus Polysaccharide from Marinc Microalga

海微藻抗艾滋病病毒多糖的结构分析及其免疫增强活性

基本信息

批准号：
07456097
负责人：
OKUTANI Koichi
金额：
$ 4.22万
依托单位：
Kagawa University
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (B)
财政年份：
1995
资助国家：
日本
起止时间：
1995 至 1996
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-07456097/
关键词：
Anti-AIDS Virus Sulfated Polysaccharide Antiviral Activites Microalga 海洋微細藻多糖

项目摘要

A marine microalga, Cochlodinium polykrikoides, produces the extracellular sulfated polysaccharide. The bacteria-free strain of C.porikrikoides was grown in the ESM medium for 50 days in continuous light at 3500 lux in a growth cabinet. Isolation and purification of the polysaccharide were accomplished by precipitation with ethanol and Cetavion, followed by DEAE-cellulose column chromatography. This polysaccharide was composed of mannose, galactose, glucose, and glucuronic acid, together with sulfate groups. The product inhibited the cytopathic effect of HIV-1, HIV-2, Zidovudine resistant HIV-1, and variuos enveloped viruses, at a similar concentrations of those of dextran sulfate, the concentrations of which were not markedly inhibitory to the blood coagulation process. The product did not inhibit the binding of HIV-1 to the host cells and the binding of anti-gp120 monoclonal antibody to HIV-1 gp120, suggesting that the mechanism of anti-HIV activity of the product may be primarily due to the inhibition of virus cell fusion. A monoclonal antibody produced by the extract of Dinoflagellate Gymnodiniu sp.reacted with the extract and cell surface of of C.polykrikoides. Therefore more precise experiments on production of monoclonal antibodies are needed for immunological identification of C.polykrikoides.

海洋微藻 Cochlodinium polykrikoides 可产生胞外硫酸多糖。 C.porikrikoides 的无菌菌株在生长柜中、在 3500 勒克斯的连续光照下、在 ESM 培养基中生长 50 天。通过用乙醇和 Cetavion 沉淀，然后通过 DEAE-纤维素柱色谱法完成多糖的分离和纯化。这种多糖由甘露糖、半乳糖、葡萄糖和葡萄糖醛酸以及硫酸基团组成。该产品在与硫酸葡聚糖相似的浓度下抑制HIV-1、HIV-2、齐多夫定耐药HIV-1和各种包膜病毒的细胞病变作用，而硫酸葡聚糖的浓度对血液凝固过程没有明显抑制作用。该产品不抑制HIV-1与宿主细胞的结合以及抗gp120单克隆抗体与HIV-1 gp120的结合，提示该产品的抗HIV活性机制可能主要是由于抑制病毒细胞融合。由裸鞭甲藻提取物与多核甲藻提取物和细胞表面反应产生的单克隆抗体。因此，需要更精确的单克隆抗体生产实验来对 C.polykrikoides 进行免疫学鉴定。