Structural Aanalyzes and the Immunostimulant Activity of the Anti-AIDS Virus Polysaccharide from Marinc Microalga

一种海洋微生物，聚克里藻，产生胞外硫酸多糖。将无菌的C.porikrikoides菌株在生长箱中在3500勒克斯的连续光照下在ESM培养基中生长50天。采用乙醇沉淀和Cetavion沉淀，再经DEAE-纤维素柱层析分离纯化多糖。该多糖由甘露糖、半乳糖、葡萄糖和葡萄糖醛酸以及硫酸酯基团组成。本品在与硫酸葡聚糖相似的浓度下抑制HIV-1、HIV-2、齐多夫定耐药HIV-1和各种包膜病毒的细胞病变效应，硫酸葡聚糖的浓度对凝血过程无显著抑制作用。该产物不抑制HIV-1与宿主细胞的结合及抗gp 120单克隆抗体与HIV-1 gp 120的结合，提示该产物抗HIV活性的机制可能主要是由于抑制病毒细胞融合。由甲藻裸甲藻提取物产生的单克隆抗体与多克里藻的提取物和细胞表面反应。因此，需要更精确的单克隆抗体的生产实验的免疫学鉴定C. polykrikoides。

A marine microalga, Cochlodinium polykrikoides, produces the extracellular sulfated polysaccharide. The bacteria-free strain of C.porikrikoides was grown in the ESM medium for 50 days in continuous light at 3500 lux in a growth cabinet. Isolation and purification of the polysaccharide were accomplished by precipitation with ethanol and Cetavion, followed by DEAE-cellulose column chromatography. This polysaccharide was composed of mannose, galactose, glucose, and glucuronic acid, together with sulfate groups. The product inhibited the cytopathic effect of HIV-1, HIV-2, Zidovudine resistant HIV-1, and variuos enveloped viruses, at a similar concentrations of those of dextran sulfate, the concentrations of which were not markedly inhibitory to the blood coagulation process. The product did not inhibit the binding of HIV-1 to the host cells and the binding of anti-gp120 monoclonal antibody to HIV-1 gp120, suggesting that the mechanism of anti-HIV activity of the product may be primarily due to the inhibition of virus cell fusion. A monoclonal antibody produced by the extract of Dinoflagellate Gymnodiniu sp.reacted with the extract and cell surface of of C.polykrikoides. Therefore more precise experiments on production of monoclonal antibodies are needed for immunological identification of C.polykrikoides.