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Immunoelectron microscopy of vasotocin neurons in the chicken hypothalamu

鸡下丘脑催产素神经元的免疫电镜观察

基本信息

批准号：
04660290
负责人：
HIRUNAGI Kanjun
金额：
$ 1.22万
依托单位：
Nagoya University
依托单位国家：
日本
项目类别：
Grant-in-Aid for General Scientific Research (C)
财政年份：
1992
资助国家：
日本
起止时间：
1992 至 1993
项目状态：
已结题

项目摘要

Vasotocin(VT)- immunoreactive(ir) neurons of the domestic chicken were studied by an immunohistochemical technique.There are several VT-ir cell populations in the preoptic areas and periventricular regions of the hypothalamus. The sizes of these cell bodies are 22-25X15mum. Shape factors are 0.7-0.8. In preoptic areas VT-ir cell bodies are distributed just under a pia mater. In the periventricular region, there are many VT-ir processes and somata. Some of these immunoreactive fibers apparently originate in each of the immunoreactive somata in the subependymal zone. Thich immunoreactive processes running toward the third ventricle seem to reach the ependymal cell layr. These processes seem to be dendritic processes of immunoreactive neurons located in the subependymal region.Immunoelectron microscopy of VT-ir neurons of the P-1 region(Berk et al. 1982)revealed VT-ir processes that make contact with the basal elements of ependymal cells of the third ventricle. On these VT-ir processes, immunonegative terminals are found. In the subependymal neuropil of the P-1 region, many immunoreactive processes of various sizes are found. Immunonegative synaptic terminals containing clear vesicles were frequently observed on thin immunopositive processes. The junctional comlex of ependymal cells seemes to lack a tight junction.Immunoelectron microscopy of neuropeptide Y(NPY) revealed that there are many NPY-ir processes and terminal like formations in the P-1 region. At least two types of NPY-ir processes. First, some of precesses are axonal fibers. Second type of processes belongs to terminals. Terminal-like structures are 725-750nm in diameter. NPY-ir axonal terminals frequently contact with immunonegative dendrites in this region. These results suggest that VT-ir neurons of P-1 region receive a NPY projection. Further studies are needed to confirm the NPY projection of this region in the domestic chicken.

采用免疫组织化学技术研究了家鸡血管催产素（VT）免疫反应神经元。在下丘脑视前区和脑室周围区有几个VT-ir细胞群。这些细胞体的大小为22-25X15mum。形状因子为0.7-0.8。视前区VT-ir细胞体分布在脑膜下。在心室周围区域，有许多VT-ir突和躯体。其中一些免疫反应纤维显然起源于室管膜下区的每个免疫反应体。向第三脑室行进的免疫反应过程似乎到达室管膜细胞层。这些过程似乎是位于室管膜下区的免疫反应神经元的树突状过程。P-1区VT-ir神经元的免疫电镜（Berk et al. 1982）显示，VT-ir过程与第三脑室室管膜细胞的基底部接触。在这些VT-ir过程中，发现了免疫负性终端。在P-1区的室管膜下神经节中，发现许多大小不等的免疫反应性突起。在薄的免疫阳性突起上经常观察到含有透明囊泡的免疫阴性突触终末。室管膜细胞的连接复合体似乎缺乏紧密的连接。神经肽Y（NPY）的免疫电镜显示，在P-1区存在许多NPY-ir过程和末端样结构。至少两种类型的NPY-ir进程。首先，一些进动是轴突纤维。第二类进程属于终端。终端状结构直径为725 ~ 750nm。NPY-ir轴突末端经常与该区域的免疫阴性树突接触。这些结果表明，P-1区的VT-ir神经元接受了NPY投射。需要进一步的研究来证实该区域在家鸡中的NPY预测。