Immuno-electron microscopic alalysis of brain photoreceptor in birds.

鸟类脑感光器的免疫电子显微镜分析。

• 批准号: 06806035
• 负责人: HIRUNAGI Kanjun
• 金额: $ 0.19万
• 依托单位: Nagoya University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for General Scientific Research (C)
• 财政年份: 1994
• 资助国家: 日本
• 起止时间: 1994 至 1995
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-06806035/
• 关键词: Pigeon; VIP neuron; GnRH neuron; Deep brain photoreceptor; Lateral septum; Immunoelectron microscopy; 髄液接触ニューロン; VIP

In the lateral septum of the avian brain, the presence of vasoactive intestinal polypeptide (VIP) -and gonadotrophin releasing hormone (GnRH) -containing neurons has been reported. An accumulation of VIP-like immunoreactive (ir) neurons is found in the area adjacent to the lateral ventricles which is called pars medialis of lateral septal organ (LSOm) and many of them displayd the characteristic features of CSF-contacting neurons. Recently, this type of neuron is considered as a candidate of deep brain photoreceptor in birds. GnRH-ir neurons are located in the deeper layr of LSOm. In the present study, we used double-labeling techniques at the light (LM) and electron-microscopic (EM) levels to investigate whether VIP-and GnRH-ir neurons communicate in the lateral septum of the pigeon. At the LM levels, VIP-ir neurons and GnRH-ir neurons were seen in close association and some VIP-ir fibers ran near GnRH-ir cell bodies. Examination of these neurons at the EM revealed that VIP-ir axon terminals had synaptoid contacts with perikarya and dendrite of GnRH-ir neurons. These results suggest that VIP neurons could communicate directly with GnRH neurons in the lateral septum of the pigeon and this area might play an important role for reproductive phenomena.

在鸟类大脑的外侧隔中，存在含有血管活性肠多肽（VIP）和促性腺激素释放激素（GnRH）的神经元。在侧隔器官内侧部（parsmedialisoflateralseptalorgan，LSOm），VIP样免疫反应阳性神经元聚集，其中许多具有接触脑脊液神经元的特征。最近，这种类型的神经元被认为是鸟类脑深部光感受器的候选者。GnRH-ir神经元位于LSOm的深层。在本研究中，我们使用双标记技术，在光（LM）和电子显微镜（EM）水平，以调查是否VIP和GnRH-ir神经元沟通在外侧隔的鸽子。在LM水平，VIP阳性神经元与GnRH阳性神经元紧密相连，并有VIP阳性纤维走行于GnRH阳性神经元胞体附近。这些神经元的EM检查显示，VIP-IR轴突终末与GnRH-IR神经元的胞体和树突有突触样接触。结果提示，鸽外侧隔区VIP神经元可与GnRH神经元直接通讯，该区域可能在鸽的生殖活动中起重要作用。

项目成果

Hirunagi, K., Kiyoshi, K., Sakakibara, K., Adachi, A., Ebihara, S., Silver, R.: "Opsin-and VIP-like immunoreactive cerebrospinal fluid (CSF) -contacting neurons in the lateral septum of the pigeon." Fourth IBRO World Congress of Neuroscience ABSTRACTS,Rap

Hirunagi, K.、Kiyoshi, K.、Sakakibara, K.、Adachi, A.、Ebihara, S.、Silver, R.：“外侧隔膜中的视蛋白和 VIP 样免疫反应性脑脊液 (CSF) 接触神经元

Hirunagi, K., Kiyoshi, K., Adachi, A., Ebihara, S., Maeda, K., Korf, H.W.: "Vasoactive intestinal peptide (VIP)-like immunoreactive cerebrospinal fluid (CSF)-contacting neurons in the avian lateral septum." Neuroendocrinology. 60, Suppl.1. 76 (1994)

Hirunagi, K.、Kiyoshi, K.、Adachi, A.、Ebihara, S.、Maeda, K.、Korf, H.W.：“鸟类中的血管活性肠肽（VIP）样免疫反应性脑脊液（CSF）接触神经元

Hirunagi, K.Rommel, E., Korf, H.-W.: "Ultrastructure of cerebrospinal fluid-contacting neurons immunoreactive to vasoactive intestinal peptide and properties of the blood-brain barrier in the lateral septal organ of the duck." Cell Tissue Res.279. 123-133

Hirunagi，K.Rommel，E.，Korf，H.-W.：“对血管活性肠肽产生免疫反应的脑脊液接触神经元的超微结构以及鸭侧隔膜器官中血脑屏障的特性。”

Ebihara,S.,Hasegawa,M,Adachi,A.,Hirunagi,K.: "Photic regulation of pineal melatonin rhythms in the pigeon." In:Evolution of Circadian Clock (Hiroshige,T.& Honma,K.eds.) Hokkaido Univ.Press,Sapporro.117-126 (1994)

Ebihara,S.、Hasekawa,M、Adachi,A.、Hirunagi,K.：“鸽子松果体褪黑激素节律的光调节。”

Hirunagi,K.,Kiyoshi,K.,Adachi,A.Ebihara,S.,Maeda,K.,Korf,H.W.: "Vasoactive intestinal peptide (VIP) -like immunoreactive cerebrospinal fluid (CSF)-contacting neurons in the avian lateral septum." Neuroendocrinology. 60,Sippl.1. -76 (1994)

Hirunagi，K.，Kiyoshi，K.，Adachi，A.Ebihara，S.，Maeda，K.，Korf，H.W.：“鸟类外侧血管活性肠肽（VIP）样免疫反应性脑脊液（CSF）接触神经元