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Morphological analysis of the cerebrospinal-fluid-(CSF)-contacting neuron in the lateral septum of avian species

鸟类外侧隔膜脑脊液（CSF）接触神经元的形态学分析

基本信息

批准号：
12660270
负责人：
HIRUNAGI Kanjun
金额：
$ 1.98万
依托单位：
Nagoya University
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (C)
财政年份：
2000
资助国家：
日本
起止时间：
2000 至 2002
项目状态：
已结题

项目摘要

We demonstrated clusters of immunoreactive small neurons in the lateral septum of the Japanese quail Opsin-like immunoreactive neurons and VIP-like immunoreactive neurons have similar morphological features. Their perikarya accumulate in the subependymal regions of the ventromedial walls of both lateral ventricles. The labelled neurons are multipolar or bipolar with pyriform or spindle-shaped cell bodies. Immunoreactive CSF-contacting neurons contact the CSF via a process that penetrates the ependyma and terminates in a single knob-like swelling. Scanning electron microscopic study revealed ventricular processes. Spherical bodies (about 2 μm in diameter) are found in a highly circumscribed region of the lateral ventricular wall of the medial lateral septum. These structures are distributed within about 1000 μm in rostro-caudal direction This structure is considerd as the venticular process of the CSF-contacting neuron. We examined immunoelectron microscopic analyses of neuronal elements in the suprachiasmtic nucleus (mSCN) of the Japanese quail using antibodies of vasoactive intestinal polypeptide (VIP), VIP receptor (VIPR) and an opsin. VIP ir elements are classified in two groups. About 40% of VIP ir neuronal elements are a synapse type. Another is a varicose type, which shows no synaptic specialization in the section. VIPR immunoreactivity is found as a fuzzy material on the membrane of the process, which seems to correspond to a synaptic junction. Opsin ir elements contain immunoreactive small granules. This neuronal element is probably a varicose of neuronal fiber. In several avian species, VIP and opsin co-exist in the deep brain photoreceptor. The presence of opsin ir terminals suggests that opsin ir deep brain photoreceptors of the lateral septum innervate the mSCN of the Japanese quail.

我们证明了在日本鹌鹑的外侧隔的Opsin样免疫反应阳性神经元和VIP样免疫反应阳性神经元的免疫反应小神经元簇具有相似的形态特征。其胞体聚集在两侧脑室腹内侧壁的室管膜下区域。标记神经元为多极或双极，胞体呈梨形或梭形。免疫反应性接触CSF的神经元通过穿透室管膜的过程接触CSF，并终止于单个结节样肿胀。扫描电子显微镜研究显示心室的过程。在内侧隔的侧心室壁的高度限制区域中发现球体（直径约2 μm）。这些结构沿吻尾方向分布在1000 μm左右的范围内，是接触脑脊液神经元的脑室突。我们研究了免疫电镜分析的神经元成分在视交叉上核（mSCN）的日本鹌鹑使用抗体的血管活性肠多肽（VIP），VIP受体（VIPR）和视蛋白。VIP IR元素分为两类。约40%的VIP免疫神经元元件是突触型。另一种是静脉曲张型，在节段中没有显示突触特化。VIPR免疫反应性被发现作为一个模糊的材料膜上的过程，这似乎对应于一个突触连接。视蛋白ir元件含有免疫反应性小颗粒。这种神经元成分可能是神经纤维的静脉曲张。在几种鸟类中，VIP和视蛋白共存于深部脑光感受器中。视蛋白IR终端的存在表明，视蛋白IR脑深部光感受器的外侧隔支配的mSCN的日本鹌鹑。