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Relation between rat early ontogenical development of hemopoietic focus and placenta.

大鼠早期造血灶个体发育与胎盘的关系。

基本信息

批准号：
04670018
负责人：
FUJIKURA Yoshihisa
金额：
$ 1.41万
依托单位：
Yamaguchi University
依托单位国家：
日本
项目类别：
Grant-in-Aid for General Scientific Research (C)
财政年份：
1992
资助国家：
日本
起止时间：
1992 至 1994
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-04670018/
关键词：
Rat Hemopoietic region Hemopoietic cell Placenta Immunohistochemistry Monoclonal antibody Culture 造血細胞

项目摘要

This research was done to clear where main hemopoiesis occurs during rat ontogenical development from yolk sac, fetal liver, fetal or neonatal spleen to bone marrow and also examined the organ, cell or factor which support the hemopoiesis. It is mentioned that hemopoietic cell, stromal cell and hemopoietic stimulating factor, are necessary to achieve hemopoiesis. We have reported UB-12 monoclonal antibody which was raised against 15days fetal liver cells, mainly hemopoietic cells, recognized rat hemopoietic cells of early stage. On the other hand, culture supernatant of placenta is usually used as a source of hemopoietic stimulating factor in vitro assay (CFU-C) . In this study, we analyzed the relation between hemopoietic stromal cells and stimulating factor.We showed that UB-12 antigen was expressed on placenta tissue by immunofluorescence method. It has been suggested that placenta plays an important role in hemopoiesis of rat. We tried to make a cell line from rat placentatissue, b … More ut could not obtain. We isografted dissociated hepatocytes which were prepared from collagenase perfused rat liver to spleen, salivary gland, thymus or subcapsular region of kidney of partially hepatectomized rats. The transplanted hepatocytes formed liver tissue heterotopically, which had 3 faces of hepatocytes, such as sinusoidal, bile-canalicular and contiguous faces. The results were examined by using HAM2, HAM4 and HAM8 monoclonal antibodies. When dissociated hepatocytes from fetal rat liver were transplanted heterotopically, as same as above mentioned methods, they form not only liver tissue but also duct-like structure in situ. The results suggested that fetal hepatocytes are more undifferentiated than adult rat hepatocytes. We made new monoclonal antibodies which were named UB18 and UB19, against 18-day fetal rat liver cells. These antibodies reacted to adult rat hepatocytes but not fetal rat hepatocytes. This may mean both hepatocytes expressed different cell surface antigens on each cell, and we consider that fetal rat hepatocyte is one of the candidates of hemopoietic stromal cells in rat. Less

本研究从卵黄囊、胎肝、胎脾、新生儿脾脏到骨髓，明确了大鼠个体发育过程中造血的主要发生部位，并对支持造血的器官、细胞或因子进行了研究。造血干细胞、基质细胞和造血刺激因子是实现造血所必需的。我们报道了针对15天胎肝细胞（主要是造血细胞）的UB-12单克隆抗体识别早期大鼠造血细胞。另一方面，胎盘的培养上清液通常用作体外测定造血刺激因子（CFU-C）的来源。本研究分析了造血基质细胞与造血刺激因子的关系，用免疫荧光法证实胎盘组织表达UB-12抗原。胎盘在大鼠造血过程中起重要作用。我们试图从大鼠胎盘组织中建立一个细胞系，B ...更多信息但无法获得。将胶原酶灌注大鼠肝脏制备的游离肝细胞移植到部分肝切除大鼠的脾脏、唾液腺、胸腺或肾被膜下。移植肝细胞异位形成肝组织，肝组织具有肝细胞的3个面，即肝窦面、胆管面和肝细胞的连续面。用HAM 2、HAM 4和HAM 8单克隆抗体检测结果。将胎鼠肝游离肝细胞异位移植，与上述方法相同，既可原位形成肝组织，又可原位形成导管样结构。结果表明，胎鼠肝细胞比成年大鼠肝细胞更未分化。我们制备了新的抗18天胎鼠肝细胞的单克隆抗体，命名为UB 18和UB 19。这些抗体与成年大鼠肝细胞反应，但不与胎鼠肝细胞反应。这可能意味着两种肝细胞表达不同的细胞表面抗原，我们认为胎鼠肝细胞是大鼠造血基质细胞的候选细胞之一。少