Relation between rat early ontogenical development of hemopoietic focus and placenta.

本研究旨在明确大鼠个体发育过程中，从卵黄囊、胎肝、胎儿或新生儿脾到骨髓的主要造血发生部位，以及支持造血的器官、细胞或因素。其中提到，造血细胞、基质细胞和造血刺激因子是实现造血所必需的。我们报道了针对15日龄胎肝细胞(主要是造血细胞)制备的识别早期大鼠造血细胞的UB-12单抗。另一方面，胎盘培养上清液通常被用作体外造血刺激因子(CFU-C)的来源。在本研究中，我们分析了造血基质细胞与刺激因子的关系，用免疫荧光方法证明UB-12抗原在胎盘组织中表达。已有研究表明胎盘在大鼠的造血过程中起重要作用。我们试图从大鼠胎盘组织中建立一种细胞系，b…。无法获得更多的UT。我们将胶原酶制备的游离肝细胞移植到部分肝切除大鼠的脾、唾液腺、胸腺或肾被膜下区域。移植的肝细胞异位形成肝组织，肝细胞有肝窦、胆管和毗邻三面。用HAM2、HAM4和HAM8三种单抗进行检测。与上述方法相同，分离的胎鼠肝细胞异位移植时，不仅可在原位形成肝组织，还可形成管状结构。结果表明，胎鼠肝细胞比成年大鼠肝细胞更未分化。我们制备了新的抗胎鼠肝细胞的单抗，命名为UB18和UB19。这些抗体可与成年大鼠肝细胞反应，但不与胎鼠肝细胞反应。这可能意味着两种肝细胞在每个细胞上表达不同的细胞表面抗原，我们认为胎鼠肝细胞是大鼠造血基质细胞的候选细胞之一。较少

This research was done to clear where main hemopoiesis occurs during rat ontogenical development from yolk sac, fetal liver, fetal or neonatal spleen to bone marrow and also examined the organ, cell or factor which support the hemopoiesis. It is mentioned that hemopoietic cell, stromal cell and hemopoietic stimulating factor, are necessary to achieve hemopoiesis. We have reported UB-12 monoclonal antibody which was raised against 15days fetal liver cells, mainly hemopoietic cells, recognized rat hemopoietic cells of early stage. On the other hand, culture supernatant of placenta is usually used as a source of hemopoietic stimulating factor in vitro assay (CFU-C) . In this study, we analyzed the relation between hemopoietic stromal cells and stimulating factor.We showed that UB-12 antigen was expressed on placenta tissue by immunofluorescence method. It has been suggested that placenta plays an important role in hemopoiesis of rat. We tried to make a cell line from rat placentatissue, b … More ut could not obtain. We isografted dissociated hepatocytes which were prepared from collagenase perfused rat liver to spleen, salivary gland, thymus or subcapsular region of kidney of partially hepatectomized rats. The transplanted hepatocytes formed liver tissue heterotopically, which had 3 faces of hepatocytes, such as sinusoidal, bile-canalicular and contiguous faces. The results were examined by using HAM2, HAM4 and HAM8 monoclonal antibodies. When dissociated hepatocytes from fetal rat liver were transplanted heterotopically, as same as above mentioned methods, they form not only liver tissue but also duct-like structure in situ. The results suggested that fetal hepatocytes are more undifferentiated than adult rat hepatocytes. We made new monoclonal antibodies which were named UB18 and UB19, against 18-day fetal rat liver cells. These antibodies reacted to adult rat hepatocytes but not fetal rat hepatocytes. This may mean both hepatocytes expressed different cell surface antigens on each cell, and we consider that fetal rat hepatocyte is one of the candidates of hemopoietic stromal cells in rat. Less