The whole aspect elucidation of gap junction formation process between adjecent cells using the anti-connexin 32 monoclonality antibody.
使用抗连接蛋白 32 单克隆抗体全面阐明相邻细胞之间间隙连接的形成过程。
基本信息
- 批准号:10470006
- 负责人:
- 金额:$ 7.74万
- 依托单位:
- 依托单位国家:日本
- 项目类别:Grant-in-Aid for Scientific Research (B).
- 财政年份:1998
- 资助国家:日本
- 起止时间:1998 至 2000
- 项目状态:已结题
- 来源:
- 关键词:
项目摘要
1. The production of HAM12 antibody hich recognizes Cx32 extacellular epitope. BALB/c mice were immnunaized by the mixture of syuthetized peptides and Freunt Complete Adjuvant. The peptide was identical to the 2nd extracellular loop of connexin 32 from p160-172 and it was labeled with diphtheria toxoid at the Cys position.After chicking whethere the plasma cell in the mouse were producing the antibody, cell fusion of NS- 1 myeloma cells and spleen cells from immunized mouse was done, then the hybridomas are under cloning now.2. The analysis of the Cx32 formation process of the rat hepatocyte in vivo.The liver frozen sections from HAM8 antibody injected rats were staind with only FITC-labeled anti-mouse IgG.As a result of observing the slice by fluorescence microscope, the formation process of the gap junction spot was estimated at the formation speed, and the hypothesis was made. The details were appeared Anat Rec 262 : 147-152, 20013. The analysis of the Cx32 formation process of the rat hepatocyte in vitro.Though primary culture method of the dissociated heachievement-finished, the achievement is being carried out by conformer and under the differential interference fluorescence microscope which inverts the micromanuplation technique at present too.
1.产生识别Cx 32胞外表位的HAM 12抗体。用合成肽与弗氏完全佐剂混合免疫BALB/c小鼠。结果:1.该肽与p160-172的连接蛋白32胞外第二环相同,并在Cys位置用白喉类毒素标记,在小鼠浆细胞产生抗体的情况下,将免疫小鼠的脾细胞与NS- 1骨髓瘤细胞进行细胞融合,目前杂交瘤正在克隆化中.大鼠肝细胞Cx 32在体内形成过程的分析将注射HAM 8抗体的大鼠肝冰冻切片,仅用FITC标记的抗小鼠IgG染色,通过荧光显微镜观察切片,以形成速度估计差距连接点的形成过程,并提出假说。详细信息见Anat Rec 262:147-152,20013。体外大鼠肝细胞Cx 32形成过程的分析,通过游离肝细胞的原代培养方法,完成了目前倒置显微操作技术,在构象异构仪和微分干涉荧光显微镜下的研究。
项目成果
期刊论文数量(64)
专著数量(0)
科研奖励数量(0)
会议论文数量(0)
专利数量(0)
F.Tamura, M.Masahara, I.Sakaida, T.Fukumoto, T.Nakamura, K.Okita: "FK506 promotes liver regeneration by suppressing natural killer cell activity."J.Gastroenterol Hepatol.. 12. 1-6 (1998)
F.Tamura、M.Masahara、I.Sakaida、T.Fukumoto、T.Nakamura、K.Okita:“FK506 通过抑制自然杀伤细胞活性促进肝脏再生。”J.Gastroenterol Hepatol.. 12. 1-6 (1998
- DOI:
- 发表时间:
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- 影响因子:0
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- 通讯作者:
Katsube K.et al.: "Successful nerve regeneration and persistence of donor cells after a limited course of immunosuppression in rat peripheral nerve allografts."Transplantation. 66. 772-777 (1989)
Katsube K.等人:“在大鼠周围神经同种异体移植物中,经过有限疗程的免疫抑制后,供体细胞成功再生并持续存在。”移植。
- DOI:
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- 影响因子:0
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- 通讯作者:
N.Iizuka, S.Hazama, K.Hirose, T.Abe, N.Tokuda, T.Fukumoto, A.Tangoku, M.Oka: "Interleukin-1 receptor antagonist mRNA expression and the progression of gastric carcinoma."Cancer Let.. 142. 179-184 (1999)
N.Iizuka、S.Hazama、K.Hirose、T.Abe、N.Tokuda、T.Fukumoto、A.Tangoku、M.Oka:“白细胞介素 1 受体拮抗剂 mRNA 表达与胃癌的进展。”Cancer Let
- DOI:
- 发表时间:
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- 影响因子:0
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Ito H.et al.: "Bidirectional blokade of CD4 and MHC class ll molecules an effective immunosuppressive treatment in the mouse heart transplantation model."J.Heart Lung transplant.. 17. 460-469 (1998)
Ito H.等人:“CD4 和 MHC II 类分子的双向阻断是小鼠心脏移植模型中有效的免疫抑制治疗。”J.心肺移植.. 17. 460-469 (1998)
- DOI:
- 发表时间:
- 期刊:
- 影响因子:0
- 作者:
- 通讯作者:
Zhongyu Liu: "Analysis of gap junction formation in rat hepatocytes by intravenous injection of an anti-connexin monoclonal antibody(HAM8)."Anat.Rec.. 262. 147-152 (2001)
刘忠宇:“静脉注射抗连接蛋白单克隆抗体(HAM8)分析大鼠肝细胞间隙连接形成。”Anat.Rec.. 262. 147-152 (2001)
- DOI:
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- 影响因子:0
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FUJIKURA Yoshihisa其他文献
FUJIKURA Yoshihisa的其他文献
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{{ truncateString('FUJIKURA Yoshihisa', 18)}}的其他基金
Development of formaldehyde alternative liquid (aqueous N-vinyl-2-pyrolidone) for organ preservation and tissue fixation
用于器官保存和组织固定的甲醛替代液(水性N-乙烯基-2-吡咯烷酮)的开发
- 批准号:
22590177 - 财政年份:2010
- 资助金额:
$ 7.74万 - 项目类别:
Grant-in-Aid for Scientific Research (C)
Irsogladine malate : Effect in cataracta diabetica and progress control mechanism from the viewpoint of the gap junction.
苹果酸伊索拉定:从间隙连接的角度对糖尿病白内障的作用和进展控制机制。
- 批准号:
13671843 - 财政年份:2001
- 资助金额:
$ 7.74万 - 项目类别:
Grant-in-Aid for Scientific Research (C)
Analysis of Connexin 32 Antigen Expression in Experimentally Degenerative Peripheral Nerve Using A Monoclonal Antibody.
使用单克隆抗体分析实验性退行性周围神经中的连接蛋白 32 抗原表达。
- 批准号:
07670018 - 财政年份:1995
- 资助金额:
$ 7.74万 - 项目类别:
Grant-in-Aid for Scientific Research (C)
Relation between rat early ontogenical development of hemopoietic focus and placenta.
大鼠早期造血灶个体发育与胎盘的关系。
- 批准号:
04670018 - 财政年份:1992
- 资助金额:
$ 7.74万 - 项目类别:
Grant-in-Aid for General Scientific Research (C)
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