权益分类	功能权益	普通用户	{{item.name}}会员
{{category.name}}	{{benefitItem.name}}

Irsogladine malate : Effect in cataracta diabetica and progress control mechanism from the viewpoint of the gap junction.

苹果酸伊索拉定：从间隙连接的角度对糖尿病白内障的作用和进展控制机制。

基本信息

批准号：
13671843
负责人：
FUJIKURA Yoshihisa
金额：
$ 2.05万
依托单位：
OITA UNIVERSITY
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (C)
财政年份：
2001
资助国家：
日本
起止时间：
2001 至 2003
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-13671843/
关键词：
Rat Gap junction Connexin 32 HAM8 antibody Intercelluler communication Streptozotocin Diabetes mellitus コネクション32

项目摘要

1)The establishment of diabetes mellitus model animal.Of the medicine, Streptozotocin (STZ) which causes the diabetes mellitus to male SD strain rats of the 6〜8 week-old, 6mg/0.1ml/100g, it was injected into vein at the fraction of the body weight The blood glucose level of the animals rose 2 days after the, injection, and the albidus of the crystalline lens was recognized at the 2〜3 month, and the cataract was able to be induced. The macroscope findings were photographed by the newly purchased digital camera, and the results were preserved. The animals were fixed with 3% of paraformaldehyde by low pressure perfusion, and it was made to be post-experimental material that they were frozen or fixed in the alcohol, after retina and crystalline lens were extracted.2)Experimental groupsIrsogladine malate (IM), 6.7μg/0.3ml/100g body weight of rat, was dissolved in water, and 1 time in every day was administered orally. Diet and water except for the medicine were taken ad libitum and prepared … More following.4 groups as experimental group.(1)IM was administered every day for I week, then STZ was injected.(2)STZ was once injected intravenously, then IM was administered every day for 1 week.(3)STZ was once injected intravenously, then water was administered every day for 1 week.(4)Physiological saline was once injected intravenously, then water was administered every day for 1 week.3)Analysis and resultsThe ocular tissues such as crystalline lens or retina from the experimental or normal rats were made thin sections and they were stained with andi-connexin 32,43 or 50 antibodies. But it was difficult to make sections because of the mixed existance of soft and hard tissues. Now we are trying to make the sentions, therefor we did not get the clear results.4)OtherThe organs which was affected reconstitution of gap junction such as peripheral nerve, liver, kidney, lacrimal gland and so on, were examined. Apart of data was reported in J. Electron Microsc., Prog. Diabetes Research and Med. Electron Microsc. Less

1)糖尿病模型动物的建立：给6~8周龄雄性SD大鼠静脉注射链脲佐菌素(STZ)6 mg/0.1ml/100g，按体重比例静脉注射，2天后血糖升高，2~3个月可见晶状体白化，可诱发白内障。用新购买的数码相机拍摄宏观结果，并保存结果。用3%多聚甲醛低压灌流固定动物，取出视网膜和晶状体后，冰冻或固定于酒精中，制成实验后材料。2)实验组：苹果酸伊索拉定(IM)，6.7μg/0.3ml/100g体重大鼠，溶于水中，1次/d。饮食和水，除药物外，随意服用，并制备…4组为实验组，(1)每日肌注STZ，连续1周；(2)静脉注射STZ 1次，连续1周；(3)静脉注射STZ 1次，每日注水1周；(4)静脉注射生理盐水1次，每日注水1周。3)分析与结果取实验大鼠或正常大鼠眼内晶体、视网膜等组织制作薄片，Andi-Connecexin 32、43、50抗体染色。但由于软硬组织混杂存在，切片制作困难。4)对周围神经、肝脏、肾脏、泪腺等影响缝隙连接重建的器官进行检查。部分数据发表在《电子微电子杂志》上。糖尿病研究和医学。电子显微镜。较少