脚橋被蓋核と大脳基底核出力系の分子形態学的解析

脚桥被盖核和基底神经节输出系统的分子形态分析

• 批准号: 04670042
• 负责人: 杉本 哲夫
• 金额: --
• 依托单位: Kansai Medical University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for General Scientific Research (C)
• 财政年份: 1992
• 资助国家: 日本
• 起止时间: 1992 至 无数据
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-04670042/
• 关键词: Fos発現; 大脳基底核出力系; 視床下核; 入力線維傷害; カイニン酸; MPP^+

1.線条体のカイニン酸による傷害に対応した基底核Fos発現の分析成熟雄性SDラットの右線条体前半部にカイニン酸(KA)4μgを注入し,線条体ニューロン傷害を作製した.左線条体前半部には溶媒を注入し対照とした.術後0.5,1,2,4,8,16,24,48,96時間の生存ののち潅流固定を施行.Fos免疫染色によって淡蒼球(GP),Ep,STN,黒質(SNc・SNr),視床VL核の各切片から所見を得た.KA投与動物では,KA投与側のEp,STN,SNrにおいてKA投与2時間後からFos陽性ニューロンが出現し,24時間後ので持続した.GPでは4時間後から48時間後まで観察された.一方,VLでは2時間後から8時間後まで認められた.いずれの神経核においても4時間後に最大数の細胞が標識された.一方,SNcや反対側のすべての神経核のFos陽性ニューロンは終始僅少であった.2.線条体のMPP^+による傷害に対応した基底核Fos発現の分析MPP^+25μgを右線条体前半部に注入したSDラットで,上記時間経過ののち,Fos発現を検出した,MPP^+投与動物では,4時間後に投与側のGP,Ep,STN,SNrにおいて,KA投与動物より少数のFos陽性ニューロンが一過性に出現した.3.以上の結果(1)基底核出力系は線条体ニューロン傷害に対応してFosを発現すること,(2)視床下核はFos発現に関し,これら出力系に連動した変化を呈すること,(3)線条体入力線維の傷害後,基底核出力系に発現するFosは,より軽微かつ一過性であること,が明らかになった.

1. Analysis of Fos Formation in Basal Nucleus of SD Strips by 4μg KA Injection into the Front Half of the Right Strips of SD Strips. The first half of the left stripe was injected with solvent. Fos immunostaining was performed at 0.5, 1, 2, 4, 8, 16, 24, 48 and 96 hours after operation.Fos immunostaining was performed at GP,EP,STN, SN, and VL nuclei of the optic bed.KA was administered to the animals,KA was administered to the lateral EP,STN,SNr, and KA was administered at 2 hours after administration. After 24 hours, we will continue to observe.GP will be observed after 4 hours, after 48 hours. One side,VL The maximum number of cells in the nucleus after 4 hours. Fos positive in nucleus of SNc and opposite sides of the brain was detected at the beginning of the study. 2. MPP^+ in the striated body was detected at the basal nuclei. MPP^+25μg in the striated body was detected at the beginning of the study. 4. MPP^+ in the striated body was detected at the end of the study. 3. The above results showed that (1) the basal nucleus output system was related to the occurrence of Fos in the linear injury,(2) the subretinal nucleus output system was related to the occurrence of Fos,(3) the basal nucleus output system was related to the occurrence of Fos in the linear injury,(4) the basal nucleus output system was related to the occurrence of Fos in the linear injury,(5) the basal nucleus output system was related to the occurrence of Fos in the linear injury. The sun is shining brightly.

项目成果

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Sato，Kazuo：“用低音声刺激诱导的神经元 Fos 蛋白绘制大鼠耳蜗核亚区域图。”

Ikeda,Michiko: "Distribution and cerebellar projections of cholinergic and corticotropin-releasing factor-containing neurons in the caudal vestibular nuclear complex and adjacent brainstem structures." Neuroscience. 49. 635-651 (1992)

池田美智子：“尾部前庭核复合体和邻近脑干结构中含有胆碱能和促肾上腺皮质激素释放因子的神经元的分布和小脑投射。”

Sugimoto,Tetsuo: "On the expression of Fos-like protein in the subthalamic nucleus and basal ganglia output systems induced by kainic acid injections into the rodent striatum." Neurosci.Lett.(1993)

Sugimoto,Tetsuo：“研究啮齿动物纹状体注射红藻氨酸诱导的丘脑底核和基底神经节输出系统中 Fos 样蛋白的表达。”

Sato,Kazuo: "Identification of rat brainstem sites with neuronal Fos protein induced by acoustic stimulation with pure tones." Acta Otolaryngol.(Stockh.). S500. 18-22 (1993)

Sato，Kazuo：“用纯音声刺激诱导的神经元 Fos 蛋白识别大鼠脑干部位。”