Involvement of ATP released from non-neuronal tlssues in presynaptic neuromodulation.

非神经元组织释放的 ATP 参与突触前神经调节。

基本信息

批准号：
04670132
负责人：
KATSURAGI Takeshi
金额：
$ 1.15万
依托单位：
Fukuoka University
依托单位国家：
日本
项目类别：
Grant-in-Aid for General Scientific Research (C)
财政年份：
1992
资助国家：
日本
起止时间：
1992 至 1993
项目状态：
已结题

项目摘要

The results obtained in my research project (04670132) during 1992 to 1993 are summarized as follows.Effects of inhibitors on metabolism by ectonucleotidases of ATP release from non-neuronal tissuesDegradation of exogenously applied ATP in the bathing solution with and without vas deferens and ileal longitudinal muscles of guinea pigs were measured by luciferin-luciferase assay. In medium with a suspended tissue, the amount of ATP in the bathing solution decreased quickly in a time-dependent manner to zero after 15 min. The decay curves of ATP were virtually unaffected by inhibitors of ecto-ATPase such as FSBA and DFDNB and by an ecto-5'-nucleotidase inhibitor, alpha, beta-methylene ADP.In addition, decay curves of ATP released from vas deferens and ileal segment in the presence of noradrenaline and bethanechol, respectively, were unchanged by inhibitors of these ectoenzymes except DFDNB.DFDNB seems to inhibit the release of ATP evoked by the secretagogues from these tissues.Cholinergi … More c neuromodulation evoked by ATP released from a non-neuronal tissueEffects of alpha, beta-methylene ATP (alpha, beta-mATP,a P_<2*>-receptor agonist) on endogenous ACh release evoked by electrical nerve stimulation were evaluated in guinea pig ileal longitudinal muscles. Release of ACh was measured with an HPLC-ECD system. Electrically evoked ACh release was reduced by alpha, beta-mATP at concentrations of 3 ad 30 muM.The reduction by alpha, beta-mATP (30 muM) was not observed in the presence of theophylline (100 muM), a P_1-receptor antagonist. The ATP analog induced the release of ATP in a suramin (P_2-receptor antagonist) -sensitive but Ca^<2+>-and atropine-insensitive manner, suggesting P_2-receptor-mediated release of ATP from the smooth muscle. We conclude from these findings that alpha, beta-mATP dose not reduce ATP release by direct stimulation of presynaptic P_1-receptors, and that endogenous ATP released postjunctionally by the ATP analog is decomposed metabolically to adenosine in the synaose and this adenosine triggers P_1-purinoceptor-sensitive neuromodulation of cholinergic transmission. Less

总结了我的研究项目（04670132）在1992年至1993年期间获得的结果，如下所示。通过非神经组织释放的ATP释放的抑制剂对代谢的抑制剂对非神经组织释放的Ectonucleotidass s释放的非神经组织释放的抑制酶，该抑制剂在非源自ATP的非神经组织基因中通过vas deferens deferens deferens deferens poderustruith waysruith deferensial muscrental muscnalial muscnital musciminal muscimcimcrantimal superepradement。荧光素卢西酶测定法。在具有悬浮组织的培养基中，沐浴溶液中的ATP量以时间依赖的方式迅速减少至15分钟后的零。 The decay curves of ATP were virtually unaffected by inhibitors of ecto-ATPase such as FSBA and DFDNB and by an ecto-5'-nucleotidase inhibitor, alpha, beta-methylene ADP.In addition, decay curves of ATP released from vas deferens and ileal segment in the presence of noradrenaline and betterchol, respectively, were unchanged by除dfdnb.dfdnb以外，这些外染料酶的抑制剂似乎抑制了这些组织中的促分泌物诱发的ATP的释放。胆碱酯…胆碱酯…ATP从Alpha，beta-methylene atp a ppa-apa-apa-pa-pa-pa-pa-pa-pa-matpppa中释放出的ATP引起的更多c神经调节，激动剂）在豚鼠纵向肌肉中评估了通过电神经刺激释放的内源性ACH。用HPLC-ECD系统测量ACH的释放。通过alpha，β-MATP的浓度为3 AD 30妈妈，电诱发的ACH释放降低。在存在P_1-1-Receptor拮抗剂的存在下，Alpha，Beta-MATP（30 MUM）的降低未观察到。 ATP模拟诱导了ATP在Suramin（P_2受体拮抗剂）敏感但Ca^<2+>和对阿托品不敏感的方式中释放，这表明P_2受体介导的ATP从平滑肌中释放出来。我们从这些发现中包括Alpha，β-MATP剂量不会通过直接刺激突触前P_1受体抑制ATP释放，并且ATP类似物在ATP类似物后释放的内源性ATP在Synaose和Syenosine p_1-P_1-Purinocter-Serneromodeforemodectulation in Neuromodectulation synnaose中分解为腺苷。较少的