Novel characteristics of ATP as a potential neuro-co-transmitter.

ATP 作为潜在神经递质的新特征。

基本信息

批准号：
61570114
负责人：
KATSURAGI Takeshi
金额：
$ 1.28万
依托单位：
Fukuoka University,School of Medicine
依托单位国家：
日本
项目类别：
Grant-in-Aid for General Scientific Research (C)
财政年份：
1986
资助国家：
日本
起止时间：
1986 至 1987
项目状态：
已结题

项目摘要

Recent findings provide evidence that ATP is capable of acting as a neuro-co-transmitter along with the classical transmitter,noradrenaline(NA)in adrenergically dominant tissues.The research project from 1986 to 1987 was designed to clarify further characteristics of cotransmission of NA and ATP from guinea-pig vas deferens.The study with HPLC-ECD in the first year disignated that ouabain(100<micrn>and/or monesin(10<micrn>),but not high KCI,produced a prominent liberation of granular NA which was mediated by intracellular Ca^<2+> originating, presumably,from mitochondrial store sites.In addition to measuring this NA release.the investigation in 1987 was to measure ATP released from the tissue by a lumicounting method in the presence of secretagogues such as ouabain plus monensin and high KCI.Ouabain plus monensin caused a moderate outflow of atp which was markedly diminished by both 6-hydroxydopamine and prazosin,thus,meaning primarily an extraneuronal release of the nucleotide.On the other hand,an outstanding liberation of ATP by high KCI was highly decreased by 6-hydroxydopamine or Ca^<2+>-removal,but not by prazosin,indicating,mainly a neuronal ATP release.Amounts of neuronal release of ATP by ouabain plus monensin and by high KCl were 1.7<micrn>g and 11.2<micrn>g/g wet weight,respectively.On the other hand,that of neuronal release of NA by the same procedures amounted to 7.7<micrn>g and 1.7<micrn>g/g wet weight,respectively.These results strongly support a possibility that NA and ATP are co-released from two different store sites of the adrenergic nerves by depolarization and elevation of intracellular Na^+ levels.

最近的发现提供了证据表明，ATP能够充当神经co释放器，以及经典的发射器，在肾上腺素上占主导地位的组织中的经典发射器，去甲肾上腺素（NA）。1986年至1987年的研究项目旨在阐明NA和ATP从Guinea-Pig Vas Deferensiated进行的NA和ATP的进一步特征。 ouabain（100 <micron>和/或monesin（10 <micrn>），但不是很高的kci，产生了颗粒状Na的明显解放，是由细胞内Ca^<2+>介导的，它是从线粒体商店位点起源于线粒体商店位点的。在该方法中，该方法是通过衡量的。 Ouabain Plus Monensin和High Kci.ouabain Plus Monensin引起了中等的ATP流出，这被6-羟基多巴胺和prazosin显着减少，因此，这意味着核苷酸的外交释放，主要是在另一只手中，高高的KCI降低了ATP的杰出释放，高度降低了6-Hydrox。 ca^<2+> - 删除，但不通过prazosin删除，主要指示神经元ATP释放。由Ouabain Plus Plus Monensin和High KCl的神经元释放ATP的释放，分别为1.7 <mircrn> g和11.2 <mircn> g/g g/g w湿重量。分别为1.7 <micrn> g/g湿重。这些结果强烈支持Na和ATP通过去极化和细胞内Na+水平的升高从肾上腺神经的两个不同商店共同发行的可能性。