Novel characteristics of ATP as a potential neuro-co-transmitter.
Novel characteristics of ATP as a potential neuro-co-transmitter.
批准号:
61570114
负责人:
KATSURAGI Takeshi
金额:
$1.28万
依托单位国家:
日本
项目类别:
Grant-in-Aid for General Scientific Research (C)
财政年份:
1986
资助国家:
日本
项目状态:
已结题
起止时间:
1986 至 1987
中文摘要
近年来的研究表明,ATP在肾上腺素能优势组织中与经典递质去甲肾上腺素(NA)一起作为沿着神经共递质发挥作用。1986 ~ 1987年的研究旨在进一步阐明NA和ATP在豚鼠输精管中的共传递特征。第一年的HPLC-ECD研究表明,哇巴因(100<micrn>)和(或)莫能菌素(10<micrn>),但高KCl,产生了颗粒状NA的显著释放,这是由细胞内Ca^2+介导的,据推测,除了测量这种NA释放外,1987年的研究是在存在促分泌剂如哇巴因加莫能菌素和高KCl的情况下通过发光计数法测量从组织释放的ATP。6-羟基多巴胺和哌唑嗪均能显著降低ATP的释放,因此,这主要意味着神经元释放核苷酸。另一方面,高KCl引起的ATP释放被6-羟基多巴胺或Ca^2+去除显著降低,但哌唑嗪不降低,这主要表明神经元释放ATP。哇巴因加莫能菌素和高KCl引起的神经元释放ATP的量分别为1.7 <micrn>g和11.2 <micrn>g/g湿重。另一方面,相同程序引起的神经元释放NA的量分别为7.7 <micrn>g和1.7 <micrn>g/g湿重。这些结果有力地支持了这样一种可能性,即NA和ATP是通过去极化和细胞内Na^+水平升高从肾上腺素能神经的两个不同储存部位共同释放的。
英文摘要
Recent findings provide evidence that ATP is capable of acting as a neuro-co-transmitter along with the classical transmitter,noradrenaline(NA)in adrenergically dominant tissues.The research project from 1986 to 1987 was designed to clarify further characteristics of cotransmission of NA and ATP from guinea-pig vas deferens.The study with HPLC-ECD in the first year disignated that ouabain(100<micrn>and/or monesin(10<micrn>),but not high KCI,produced a prominent liberation of granular NA which was mediated by intracellular Ca^<2+> originating, presumably,from mitochondrial store sites.In addition to measuring this NA release.the investigation in 1987 was to measure ATP released from the tissue by a lumicounting method in the presence of secretagogues such as ouabain plus monensin and high KCI.Ouabain plus monensin caused a moderate outflow of atp which was markedly diminished by both 6-hydroxydopamine and prazosin,thus,meaning primarily an extraneuronal release of the nucleotide.On the other hand,an outstanding liberation of ATP by high KCI was highly decreased by 6-hydroxydopamine or Ca^<2+>-removal,but not by prazosin,indicating,mainly a neuronal ATP release.Amounts of neuronal release of ATP by ouabain plus monensin and by high KCl were 1.7<micrn>g and 11.2<micrn>g/g wet weight,respectively.On the other hand,that of neuronal release of NA by the same procedures amounted to 7.7<micrn>g and 1.7<micrn>g/g wet weight,respectively.These results strongly support a possibility that NA and ATP are co-released from two different store sites of the adrenergic nerves by depolarization and elevation of intracellular Na^+ levels.
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Takeshi Katsuragi: "Clonidie-evoked selective P_1-purinoceptor antagonism of contraction of guinea-pig urinary bladder." European Journal of Pharmacology. 121. 119-122 (1986)
Takeshi Katsuragi:“克罗尼迪引起的豚鼠膀胱收缩的选择性 P_1-嘌呤受体拮抗作用。”
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Takeshi Katsuragi: "Synergism between ouabain and monensin in neurogenic responses of guinea-pig Vas deferens." Canadian Journal of Physiology and Pharmacology. (1988)
Takeshi Katsuragi:“哇巴因和莫能菌素在豚鼠输精管神经源性反应中的协同作用。”
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Takeshi Katsuragi: "Extra and intracellular Ca^<2+>-dependent co-releases of norepinephrine and ATP occur from two different store sites of adrenergic nerve terminals." Journal of Pharmacology and Experimental Therapeutics.
Takeshi Katsuragi:“去甲肾上腺素和 ATP 的细胞外和细胞内 Ca^2 依赖性共同释放发生在肾上腺素能神经末梢的两个不同存储位点。”
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Toshiro Harada: J.Pharmacol.Exp.Therap.239. 554-558 (1986)
原田敏郎:J.Pharmacol.Exp.Therap.239。
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共 6 条
Identification of a specific transporter on extracellular release of ATP
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批准号:17590234
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项目类别:Grant-in-Aid for Scientific Research (C)
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资助金额:$2.3万
-
财政年份:2005
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负责人:KATSURAGI Takeshi
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依托单位:
Involvement of a transporter in an autocrine / paracrine release of ATP.
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批准号:15590243
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项目类别:Grant-in-Aid for Scientific Research (C)
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资助金额:$2.18万
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财政年份:2003
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负责人:KATSURAGI Takeshi
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依托单位:
ATP-autocrine/paracrine release and its intracellular Ca^<2+> signals
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批准号:13670107
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项目类别:Grant-in-Aid for Scientific Research (C)
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资助金额:$1.79万
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财政年份:2001
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负责人:KATSURAGI Takeshi
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依托单位:
Possible involvement of CaィイD12+ィエD1-signaling transferred to mitochondria in release of ATP as an autacoid
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批准号:10670102
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项目类别:Grant-in-Aid for Scientific Research (C)
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资助金额:$1.34万
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财政年份:1998
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负责人:KATSURAGI Takeshi
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依托单位:
Intracellular signalling pathway involved in ATP release from isolated smooth muscle cells.
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批准号:07670127
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项目类别:Grant-in-Aid for Scientific Research (C)
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资助金额:$1.22万
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财政年份:1995
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负责人:KATSURAGI Takeshi
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依托单位:
Involvement of ATP released from non-neuronal tlssues in presynaptic neuromodulation.
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批准号:04670132
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项目类别:Grant-in-Aid for General Scientific Research (C)
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资助金额:$1.15万
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财政年份:1992
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负责人:KATSURAGI Takeshi
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依托单位:
A possible role as a neuromodulator of extraneuronally released-ATP.
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批准号:02670102
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项目类别:Grant-in-Aid for General Scientific Research (C)
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资助金额:$1.41万
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财政年份:1990
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负责人:KATSURAGI Takeshi
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依托单位:
海外基金