Involvement of a transporter in an autocrine / paracrine release of ATP.

转运蛋白参与 ATP 的自分泌/旁分泌释放。

• 批准号: 15590243
• 负责人: KATSURAGI Takeshi
• 金额: $ 2.18万
• 依托单位: Fukuoka University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2003
• 资助国家: 日本
• 起止时间: 2003 至 2004
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-15590243/
• 关键词: ATP release; Bradykinin; B_2-receptor; Inositol(1,4,5)tristhosthate; endoplasmic reticulum; MK-571; MRP transporter; cultured smooth muscle cells; NEM; 培養結腸紐平滑筋細胞; ATP放出機構; カフェイン刺激; 低浸透圧刺激; Ca^<2+>シグナル; ミトコンドリア; ATP合成

Bradykinin induces the release of ATP from cultured taenia coli smooth muscle cells from guinea pigs. The evoked release was prohibited by a B2-receptor antagonist (HOE-140) and (inhibitors (U-73122 and thapsigargin) for the phospholipase C - inositol (1,4,5) P_3 signal pathway. The release of ATP was interfered, with MRP inhibitors (NM-571 and benzbromarone). However, hemichann el blockers (gap26) and the blockers (glybemclamid and Gd^<3+>) of Cl channels coupled with activation of CFTR failed to attenuate the evoked release. The release of ATP by hadykinin was also inhibited by cytochalasin D, staurosporine and NEM, but not by inhibitors for Golgi body (brefeldin A) and mitochondria (oligomycin). Therefore, we conclude that the bradykinini-evoked release of ATP may be mediated by the membrane MRP transporter activated by intracellular ATP released from endoplasmic reticulum,

Bradyinin诱导从豚鼠中培养的Taenia大肠杆菌平滑肌细胞中释放ATP。 B2受体拮抗剂（HOE-140）和（抑制剂（U-73122和Thapsigargin）禁止磷脂酶C-肌醇（1,4,5）P_3 P_3信号途径。 （GAP26）和CL通道的阻滞剂（Glybemclamid和Gd^<3+>）与CFTR的激活结合使用，无法减轻诱发的释放，而Hadykinin也被CytoChalasin d，Staurosporine and Nem和bygion Brodiry（bref and bregi）抑制了Hadykinin。 （寡霉素）。

项目成果

Adenosine induces ATP release via an inositol 1,4,5-trisphosphate signaling pathway in MDCK cells

腺苷通过 MDCK 细胞中的肌醇 1,4,5-三磷酸信号通路诱导 ATP 释放

• 发表时间: 2005
• 期刊: Biochem. Biophys. Res. Commun. 328
• 作者: K.Migita et al.;K.Migita et al.
• 通讯作者: K.Migita et al.

Induction of leukotriene C_4 synthase after the differentiation of rat basophillic leukemia cells with retinoic acid and a low dose of actinomycin D and its suppression with methylprednisolone.

视黄酸和低剂量放线菌素 D 诱导大鼠嗜碱性白血病细胞分化后白三烯 C_4 合酶的诱导及其甲基强的松龙的抑制。

• 发表时间: 2003
• 期刊: J.Cell.Physiol. 196
• 作者: T.Iwamoto;S.Kita;S.Ueno et al.;M.Abe et al.
• 通讯作者: M.Abe et al.

Harada, N. et al.: "Contribution of capsaicin-sensitive sensory neurons to stress-induced increase in gastric tissue levels of prostaglandins in rats."Am J Physiol (Gastrointest Liver Physiol). 285. G1214-G1224 (2003)

Harada, N. 等人：“辣椒素敏感的感觉神经元对压力诱导的大鼠胃组织前列腺素水平增加的贡献。”Am J Physiol（胃肠测试肝脏生理学）。

Extracellular Nucleotides and Nucleosides: Release, Receptor, and Physiological and Pathophysiological effects: Relating the structur of the ATP-gated ion channel-receptors to their function

细胞外核苷酸和核苷：释放、受体以及生理和病理生理效应：ATP 门控离子通道受体的结构与其功能的关系

• 发表时间: 2003
• 作者: T.Iwamoto;S.Kita;S.Ueno et al.;M.Abe et al.;S.Ueno et al.;M.Abe et al.;T.M.Eagan et al.;T.M.Eagan et al.
• 通讯作者: T.M.Eagan et al.

Induction of leukotriene C_4 synthase after the differentiation of rat basophillic leukemia cells with retinoic acid and a low dose of actinomycin D and its suppression with methylprednisolone

视黄酸和低剂量放线菌素D诱导大鼠嗜碱性白血病细胞分化后白三烯C_4合酶的诱导及其甲泼尼龙的抑制

• 发表时间: 2003
• 期刊: J. Cell. Physiol. 196
• 作者: T.Iwamoto;S.Kita;S.Ueno et al.;M.Abe et al.;S.Ueno et al.;M.Abe et al.
• 通讯作者: M.Abe et al.