Involvement of a transporter in an autocrine / paracrine release of ATP.

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Bradykinin induces the release of ATP from cultured taenia coli smooth muscle cells from guinea pigs. The evoked release was prohibited by a B2-receptor antagonist (HOE-140) and (inhibitors (U-73122 and thapsigargin) for the phospholipase C - inositol (1,4,5) P_3 signal pathway. The release of ATP was interfered, with MRP inhibitors (NM-571 and benzbromarone). However, hemichann el blockers (gap26) and the blockers (glybemclamid and Gd^<3+>) of Cl channels coupled with activation of CFTR failed to attenuate the evoked release. The release of ATP by hadykinin was also inhibited by cytochalasin D, staurosporine and NEM, but not by inhibitors for Golgi body (brefeldin A) and mitochondria (oligomycin). Therefore, we conclude that the bradykinini-evoked release of ATP may be mediated by the membrane MRP transporter activated by intracellular ATP released from endoplasmic reticulum,