Study on characteristics and function of apoprotein A1 in cholesterol gallstone.

本研究对载脂蛋白A1在胆固醇结石中的特性和功能进行了研究，获得了以下结果：(1)采用SDS-PAGE和Western印迹方法分析了载脂蛋白A在胆固醇结石中的生化特性。载脂蛋白A在胆固醇结石中的表达特征与肝胆汁相似。同时用本实验室建立的酶联免疫吸附试验对胆汁载脂蛋白进行定量测定。胆固醇结石中载脂蛋白A的含量很低。因此，胆汁载脂蛋白A可以被动员起来，防止胆固醇清除进入胆结石。(2)用ELISA法检测肝胆汁载脂蛋白A，发现胆汁载脂蛋白A1浓度与胆汁胆固醇水平显著相关。结果表明，肝胆管结石可诱导载脂蛋白A1分泌到胆汁中。(3)载脂蛋白A在胆固醇结石中的作用研究。(4)用组织免疫组织化学方法检测了胆固醇结石中载脂蛋白A1的表达，发现在胆固醇结石中载脂蛋白A1和A2存在于胆固醇层之间，这些结果为我们研究胆固醇结石的形成机制提供了重要的信息。

In this study the characteristics and function of apoprotein A1 in cholesterol gallstone were investigated, and the following results were obtained ;(1) Biochemical characteristics of apoprotein A in cholesterol gallstone was analyzed by using SDS- PAGE and Western blot methods. Characteristics of apoprotein A in cholesterol gallstone were similar to those in hepati bile. Meanwhile, biliary aporotein was quantitatively determined by using ELISA, which was established in our laboratory. The content of apoprotein A in cholesterol gallstone was very low. Thus, biliary apoprotein A could be mobililized for preventation of cholesterol nuleation without precipitation into gallstone.(2) Measurement of apoprotein A in hepatic bile was carried out by using ELISA.There was significant correlation between bilary apoprotein A1 concentration and biliary cholesterol level. According to these data, it was demonstrated that hepatic lithogrnic bile might induce apoprotein A1 secretion into bile.(3) Study on function of apoprotein A in cholesterol gallston. Under investigation.(4) Apoprotein A1 in cholesterol gallstone was detected by histoimmunostaining, and it was shown that in cholesterol gallstone apoprotein A1 and A2 exists between cholesterol layr.These results gave us many important informations on the mechanism of cholesterol gallstone formation.