权益分类	功能权益	普通用户	{{item.name}}会员
{{category.name}}	{{benefitItem.name}}

Study on characteristics and function of apoprotein A1 in cholesterol gallstone.

胆胆固醇结石中脱辅基蛋白A1的特性及功能研究

基本信息

批准号：
04670402
负责人：
MAKINO Isao
金额：
$ 1.34万
依托单位：
Asahikawa Medical College
依托单位国家：
日本
项目类别：
Grant-in-Aid for General Scientific Research (C)
财政年份：
1992
资助国家：
日本
起止时间：
1992 至 1993
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-04670402/
关键词：
cholesterol gallstone nucleation inhibition of gallstone formation biliary protein apoprotein A slice sample of gallstone homeostasis ELISA

项目摘要

In this study the characteristics and function of apoprotein A1 in cholesterol gallstone were investigated, and the following results were obtained ;(1) Biochemical characteristics of apoprotein A in cholesterol gallstone was analyzed by using SDS- PAGE and Western blot methods. Characteristics of apoprotein A in cholesterol gallstone were similar to those in hepati bile. Meanwhile, biliary aporotein was quantitatively determined by using ELISA, which was established in our laboratory. The content of apoprotein A in cholesterol gallstone was very low. Thus, biliary apoprotein A could be mobililized for preventation of cholesterol nuleation without precipitation into gallstone.(2) Measurement of apoprotein A in hepatic bile was carried out by using ELISA.There was significant correlation between bilary apoprotein A1 concentration and biliary cholesterol level. According to these data, it was demonstrated that hepatic lithogrnic bile might induce apoprotein A1 secretion into bile.(3) Study on function of apoprotein A in cholesterol gallston. Under investigation.(4) Apoprotein A1 in cholesterol gallstone was detected by histoimmunostaining, and it was shown that in cholesterol gallstone apoprotein A1 and A2 exists between cholesterol layr.These results gave us many important informations on the mechanism of cholesterol gallstone formation.

本研究对载脂蛋白A1在胆固醇性胆结石中的特点和功能进行了研究，得到以下结果：(1)采用SDS- PAGE和Western blot方法分析胆固醇胆结石载脂蛋白A的生化特征。胆固醇性胆结石载脂蛋白A的特征与肝胆汁相似。同时，采用本实验室建立的酶联免疫吸附试验（ELISA）定量测定胆道载脂蛋白。胆石中载脂蛋白A含量很低。因此，胆道载脂蛋白A可以被动员起来预防胆固醇成核而不沉淀成胆结石。(2)采用ELISA法测定肝胆汁载脂蛋白A。胆道载脂蛋白A1浓度与胆道胆固醇水平有显著相关性。这些数据表明，肝碎石胆汁可能诱导载脂蛋白A1分泌到胆汁中。(3)载脂蛋白A在胆固醇性胆结石中的作用研究。在调查之中。(4)组织免疫染色检测胆固醇胆石载脂蛋白A1，发现胆固醇胆石载脂蛋白A1与A2存在于胆固醇层之间。这些结果为我们提供了有关胆固醇胆石形成机制的许多重要信息。