The Role and Characteristics of Reductive Enzyme in Human Erythrocyte on Metabolism of Keto-bile Acid.

人类红细胞中的酶的作用和特征学对使用脱氢酸研究的酮-bile酸的减少反应，并获得了以下结果：（1）。通过Bersus等人的方法尝试了人体红细胞中的酶的部分纯化，根据对红细胞酶系统的实验中的数据，我们演示了将脱氢酸减少到酮-组C-3的位置。（二）;根据上一篇文章，它是被接受的，因为脱水酸是由Hepatic Enzymes在间接管理和代谢物中被显著地转化为婴儿的。主要代谢物（70%）为3个α、7个α-二羟基-12-酮双酸，第二个（20%）为3个α-羟基-7，12-二酮双酸和第三个（10%）胆酸。在目前的研究中，我们演示了血清未修饰的3 α-羟基-7，12 diketo胆olanoic acid在脱氢酸的间接管理下快速增加：这种事实指标的脱氢酸可能是由酶中的酶部分代谢化的，以及一种替代的代谢途径，用于脱氢酸可能存在的代谢。根据生物转化代谢物的定量分析数据，我们粗略地计算了20%和更多的被注射的脱氢酸可以减少到3个α-羟基-7，12-二酮-胆碱酸在Peripheral血中的含量。然后，酮组在C-7和C-12的位置可以通过微组片段中的Hepatic Enzyme和精确地转化为生物。（三）; Although 3-Keto--双酸可能会在结肠炎的作用下产生结肠，这种双酸已经很难检测到。这一发现可以通过酶催化剂在Erythrocyte中降低Keto组C-3位置的说明来找到。

The role and characteristics of the enzyme in human erythrocyte for reductive reaction of keto-bile acid were studied by using dehydrocholic acid, and the following results were obtained :(1). Partial purification of this enzyme in human erythrocyte has been tried by the method of Bersus et al. Based on the data from the experiment on this enzyme system of erythrocyte, we demonstrated that reduction of dehydrocholic acid was restricted to keto-group C-3 position.(2). According to previous papers, it had been accepted that dehydrocholic acid is metabolized by hepatic enzymes after intravenous administration and the metabolites were excreted into bile. Major metabolite (70%) was 3alpha, 7alpha-dihydroxy-12-keto bile acid, the second (20%) 3alpha-hydroxy-7, 12-diketo bile acid and the third (10%) cholic acid. In the present study, we demonstrated a rapid increase of serum unconjugated 3alpha-hydroxy-7, 12diketo cholanoic acid after intravenous administration of dehydrocholic acid : this fact indicates dehydrocholic acid could be partially metabolized by the enzyme in erythrocyte, and an altered pathway for metabolism of dehydrocholic acid could be present. Based on the data from quantitative analysis of biotransformed metabolites, we calcurated roughly that 20% and more of injected dehydrocholic acid could be reduced to 3alpha-hydroxy-7, 12-diketoーcholanoic acid in peripheral blood before hepatic uptake. Then, keto-groups at C-7 and C-12 position of this metabolite could be reduced by hepatic enzyme in microsome fraction, and excreted into bile.(3). Although 3-ketoーbile acids might be produced in colon by an action of intesinal bacteria, a significant amount of this bile acid has been hardly detected. This finding could be illustrated by the enzyme catalyzing the reduction of keto group at C-3 position in erythrocyte.