A study on size and action of molecular aggregates in human bile with using ultrafiltration.

利用Disporal超滤装置对人胆汁和模型胆汁中分子聚集体的大小和作用进行了研究，结果表明：1.人胆汁和模型胆汁中分子聚集体的大小和作用是一致的。在人的胆汁中，总胆汁酸的2/3存在于大聚集部分(相对分子质量：10,000以上)，1/6存在于中等聚集部分(相对分子质量：10,000-1,000)，1/6存在于小聚集部分(相对分子质量：低于1,000)。羟基的数目、位置和侧链的电荷是决定分子聚集体大小的重要因素。这些因素对测定大聚集体的优劣顺序为：含二羟基的胆汁酸；含三羟基的胆汁酸；含羟基的(β)位的胆汁酸；含(β)-羟基的胆汁酸；含二阴离子的侧链。胆汁中的胆固醇和钙可以被分子聚集体溶解。与中、小集料相比，大集料的影响程度较大。然而，随着总胆汁脂质浓度的降低，中、小集合体对钙的溶解作用变得更加重要。在胆汁中含有低浓度胆脂的胆汁中，结合钙与分子聚集体(溶解)的能力降低，形成不溶性钙盐的风险增加。因此，推测该因子可能与胆结石钙化有关。鹅去氧胆酸处理的胆汁中分子聚集体的大小分布与熊去氧胆酸处理的胆汁中的大小有显著差异。因此，这两种药物溶解胆结石的机制不可能是相同的。

The size and action of molecular aggregates in human gallbladder bile and model bile solution was studied with using disporsal ultrafiltration unit, and the following results have been shown.1. In human gallbladder bile, two third of total biliary bile acid was present in large aggregate fraction (molecular weight: above 10,000), one sixth in middle fraction (M.W.:10,000-1,000) and one sixth in small fraction (M.w.:below 1,000). The number and site of hydroxy group and electric charge of side chain were important factors to determine the size of molecular aggregate. The superiority or inferiority of these factors to determine large aggregates was the following order: bile acid with dihydroxy group>with trihydroxy group, bile acid with<alpha>-site of hydroxy group>with (beta)-site of hydroxy group, and side chain with monoanionic form > with dianionic form.2. In bile cholesterol and calcium can be dissolved by molecular aggregate. The degree was remarkable in large aggregate fraction, comparing with those in middle and small aggregate fractions. However, the dissolution of calcium by middle and small aggregate fraction gained in importance, with decreasing in total biliary lipid concentration.3. In bile with low concentration of biliary lipid, a capacity for binding calcium with molecular aggregate (dissolution) decreased, and a risk for formation of insoluble calcium salt increased. Therefore, it was presumed that this factor might be connected with calcification of gallstone. Distribution of size of molecular aggregate in bile with chenodeoxycholic acid treatment was signigicantly different from that in bile with ursodeoxycholic acid treatment. Thus, it wasnoted that mechanism of both treaments for gallstone dissolution could not be identical.