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A study on size and action of molecular aggregates in human bile with using ultrafiltration.

使用超滤研究人胆汁中分子聚集体的大小和作用。

基本信息

批准号：
61570323
负责人：
MAKINO Isao
金额：
$ 1.22万
依托单位：
Hirosaki University
依托单位国家：
日本
项目类别：
Grant-in-Aid for General Scientific Research (C)
财政年份：
1986
资助国家：
日本
起止时间：
1986 至 1987
项目状态：
已结题

项目摘要

The size and action of molecular aggregates in human gallbladder bile and model bile solution was studied with using disporsal ultrafiltration unit, and the following results have been shown.1. In human gallbladder bile, two third of total biliary bile acid was present in large aggregate fraction (molecular weight: above 10,000), one sixth in middle fraction (M.W.:10,000-1,000) and one sixth in small fraction (M.w.:below 1,000). The number and site of hydroxy group and electric charge of side chain were important factors to determine the size of molecular aggregate. The superiority or inferiority of these factors to determine large aggregates was the following order: bile acid with dihydroxy group>with trihydroxy group, bile acid with<alpha>-site of hydroxy group>with (beta)-site of hydroxy group, and side chain with monoanionic form > with dianionic form.2. In bile cholesterol and calcium can be dissolved by molecular aggregate. The degree was remarkable in large aggregate fraction, comparing with those in middle and small aggregate fractions. However, the dissolution of calcium by middle and small aggregate fraction gained in importance, with decreasing in total biliary lipid concentration.3. In bile with low concentration of biliary lipid, a capacity for binding calcium with molecular aggregate (dissolution) decreased, and a risk for formation of insoluble calcium salt increased. Therefore, it was presumed that this factor might be connected with calcification of gallstone. Distribution of size of molecular aggregate in bile with chenodeoxycholic acid treatment was signigicantly different from that in bile with ursodeoxycholic acid treatment. Thus, it wasnoted that mechanism of both treaments for gallstone dissolution could not be identical.

利用分散式超滤装置研究了人胆囊胆汁和模型胆汁溶液中分子聚集体的大小和作用，得到了以下结果： 1．在人胆囊胆汁中，总胆汁胆汁酸的三分之二以大聚集部分（分子量：10,000以上）存在，六分之一以中间部分（分子量：10,000-1,000）存在，六分之一以小部分（分子量：1,000以下）存在。羟基的数量和位置以及侧链的电荷是决定分子聚集体尺寸的重要因素。这些因素对大聚集体的优劣顺序为：胆汁酸二羟基>三羟基，胆汁酸α位羟基>β位羟基，侧链单阴离子形式>双阴离子形式。 2．胆汁中的胆固醇和钙可以通过分子聚集体溶解。与中、小骨料部分相比，大骨料部分的程度更为显着。然而，随着胆汁总脂质浓度的降低，中、小聚集部分对钙的溶解变得越来越重要。3．在胆汁脂质浓度低的胆汁中，钙与分子聚集体结合（溶解）的能力降低，并且形成不溶性钙盐的风险增加。因此推测该因素可能与胆结石钙化有关。经鹅去氧胆酸处理的胆汁中分子聚集体的尺寸分布与经熊去氧胆酸处理的胆汁中的分子聚集体大小分布显着不同。因此，人们注意到两种治疗胆结石溶解的机制不可能相同。