A Growth Inhibitor of Rat Hepatocytes Synthesized by a Rat Hepatoma Cell Line, FF101

由大鼠肝癌细胞系 FF101 合成的大鼠肝细胞生长抑制剂

• 批准号: 04670424
• 负责人: KAWASAKI Tsunehisa
• 金额: $ 1.34万
• 依托单位: Hamamatsu University School of Medicine
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for General Scientific Research (C)
• 财政年份: 1992
• 资助国家: 日本
• 起止时间: 1992 至 1993
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-04670424/
• 关键词: hepatocytes primary culture; growth factor; growth inhibitor; hepatoma; serum free culture

A rat hepatoma cell line, FF101, established in serum-free, protein-free medium synthesizes a growth inhibitor of adult rat hepatocytes in primary culture. Gel filtration of FF101-conditioned medium disclosed two peaks of growth inhibitory activity with the molecular sizes over 200kD and smaller than 10kD.The growth inhibitor of smaller molecular size was tentatively designated as FF-GI.The DNA synthesis of rat hepatocytes was inhibited by FF-GI.The inhibitory activity of FF-GI was abolished by treatments with trypsin, dithiothreitol and heating at 100ﾟC for 30min, although it was stable against acid treatment and heating at 100ﾟC for 5min.These results indicate that FF-GI may be a novel growth inhibitor of adult rat hepatocytes.

大鼠肝癌细胞系，FF 101，建立在无血清，无蛋白质的培养基合成的生长抑制剂的成年大鼠肝细胞在原代培养。FF 101条件培养液的凝胶过滤显示出两个生长抑制活性峰，其分子大小分别为200 kD和10 kD以下，小分子生长抑制剂初步命名为FF-GI。FF-GI对大鼠肝细胞DNA合成有抑制作用，用胰蛋白酶、二硫苏糖醇处理和100 ℃加热30 min可使其抑制活性消失，FF-GI对酸处理和100 ℃加热5 min均稳定，提示FF-GI可能是一种新的肝细胞生长抑制剂。

项目成果

Hirofumi Kitahara: "A growth inhibitor of rat hepatocytes synthesized by a rat hepatoma cell line,FF101" International Hepatology Communications. (掲載予定).

Hirofumi Kitahara：“由大鼠肝癌细胞系合成的大鼠肝细胞生长抑制剂，FF101”国际肝病学通讯（待出版）。

Hirofumi Kitahara: "A growth inhibitor of rat hepatocytes synthesized by a rat hepatoma cell line, FF101." International Hepatology Communications. (in press).

Hirofumi Kitahara：“由大鼠肝癌细胞系 FF101 合成的大鼠肝细胞生长抑制剂。”