The Evaluation of Photodynamic therapy (PDT) for cancer with ^<31>P nuclear magnetic resonance spectroscopy.

1. 光动力治疗（PDT）后的^<31>p核磁共振波谱（^<31> p MRS）与组织病理学结果进行比较。将Ph-Il （Photofrin）或ATX-S10腹腔注射BALB/c裸鼠。在Phil注射24小时或ATX-S10注射2小时后，用YAGOPO激光器将移植的HeLa细胞肿瘤完全照射到50-100J/肿瘤，其中Ph-II产生630 nm的光，ATX-S10产生670 nm的光。在PDT后1、3、7天测量^<31>p MRS，每天对标本进行组织病理学研究。在ATX-S10 PDT后第1天，肿瘤的^<31>p MRS中ATP几乎消失，活的HeLa细胞在其中无法检测到。相比之下，当活细胞存在时，相当数量的ATP被识别出来。在Ph- II型PDT中，PDT后1天ATP减少与肿瘤根除量无关，PDT后7天总磷酸盐减少与HeLa活细胞减少有关。此外，我们观察到ATX- S10 PDT后1天损伤细胞完全死亡，而Ph-LI后细胞未完全死亡。p - MRS是评价PDT效果和研究其作用机制的一种非常有用的方法。为了研究光敏剂在实验性肝肿瘤中的分布，我们用二乙基亚硝基胺诱导大鼠肝脏肿瘤病变11周。在该模型中观察到增生性结节、非典型结节和肝细胞癌等交界性病变。注射δ -氨基乙酰丙酸（ALA），在给药后1,2,3,4,6,8 h用光诱导荧光检测系统检测原卟啉inix （PpIX）荧光。ALA给药3小时后，PpIX荧光在癌组织中比在正常组织中更明显。ALA可能在实验性肝癌中选择性积累。

1. The^<31>p nuclear magnetic resonance spectroscopy (^<31>P MRS ) measured after photodynamic therapy (PDT) were compared with the histopathological findings. Ph-Il (Photofrin) or ATX-S10 were intraperitoneally injected to BALB/c nude mice. 24 hours after Phil injection or two hours after the ATX-S10 injection the transplanted HeLa cell tumor was totally irradiated to 50-100J/tumor by YAGOPO laser which generates a light at a wavelength of 630 nm for Ph-II and 670 nm for ATX-S 10. The^<31>p MRS was measured 1, 3 and 7 days after PDT and the specimens were histopathologically studied each day. On the 1st day after ATX-S10 PDT, ATP almost disappeared in the^<31>p MRS of the tumor when live HeLa cells were undetectable in it. Contrastively, a considerable amount of ATP was recognized when live cells were remained. In Ph- II PDT, ATP decrease 1 day after PDT didn't correlate to the eradicated tumor volume, and the total phosphate decrease up to 7 days after PDT correlated to the decreasing live HeLa cells. Moreover, we observed one day after ATX- S10 PDT the damaged cells were completely dead, however the cells after Ph-LI were not comletely dead.31P MRS is a very useful method to evaluate the effects of PDT and to investigate its mechanisms.2. In order to investigate the distribution of photosensitizer in the experimental liver tumor, neoplastic hepatic lesions were induced in rats given diethylnitrosoamine for 11 weeks. Borderline lesions like hyperplastic nodule and atypical nodule, and hepatocellular carcinoma were observed in this model. delta -aminolevulinic acid (ALA) was injected and protoporphyrinIX (PpIX) fluorescence was measured using light induced fluorescence detection system 1,2,3,4,6,8 hours after administration. Three hours after ALA administration, PpIX fluorescence tended to be greater in cancer than in normal tissure.ALA might accumulate selectively in experimental liver cancer.