权益分类	功能权益	普通用户	{{item.name}}会员
{{category.name}}	{{benefitItem.name}}

The Evaluation of Photodynamic therapy (PDT) for cancer with ^<31>P nuclear magnetic resonance spectroscopy.

用 31 P核磁共振波谱法评估癌症光动力疗法(PDT)。

基本信息

批准号：
09670534
负责人：
KAWASAKI Tsunehisa
金额：
$ 1.47万
依托单位：
Hamamatsu University School of Medicine
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (C)
财政年份：
1997
资助国家：
日本
起止时间：
1997 至 1998
项目状态：
已结题

项目摘要

1. The^<31>p nuclear magnetic resonance spectroscopy (^<31>P MRS ) measured after photodynamic therapy (PDT) were compared with the histopathological findings. Ph-Il (Photofrin) or ATX-S10 were intraperitoneally injected to BALB/c nude mice. 24 hours after Phil injection or two hours after the ATX-S10 injection the transplanted HeLa cell tumor was totally irradiated to 50-100J/tumor by YAGOPO laser which generates a light at a wavelength of 630 nm for Ph-II and 670 nm for ATX-S 10. The^<31>p MRS was measured 1, 3 and 7 days after PDT and the specimens were histopathologically studied each day. On the 1st day after ATX-S10 PDT, ATP almost disappeared in the^<31>p MRS of the tumor when live HeLa cells were undetectable in it. Contrastively, a considerable amount of ATP was recognized when live cells were remained. In Ph- II PDT, ATP decrease 1 day after PDT didn't correlate to the eradicated tumor volume, and the total phosphate decrease up to 7 days after PDT correlated to the decreasing live HeLa cells. Moreover, we observed one day after ATX- S10 PDT the damaged cells were completely dead, however the cells after Ph-LI were not comletely dead.31P MRS is a very useful method to evaluate the effects of PDT and to investigate its mechanisms.2. In order to investigate the distribution of photosensitizer in the experimental liver tumor, neoplastic hepatic lesions were induced in rats given diethylnitrosoamine for 11 weeks. Borderline lesions like hyperplastic nodule and atypical nodule, and hepatocellular carcinoma were observed in this model. delta -aminolevulinic acid (ALA) was injected and protoporphyrinIX (PpIX) fluorescence was measured using light induced fluorescence detection system 1,2,3,4,6,8 hours after administration. Three hours after ALA administration, PpIX fluorescence tended to be greater in cancer than in normal tissure.ALA might accumulate selectively in experimental liver cancer.

1.光动力疗法（PDT）后测量的^<31>P核磁共振波谱（^<31>P MRS）与组织病理学结果进行比较。将Ph-Il（Photofrin）或ATX-S10腹膜内注射至BALB/c裸鼠。在Phil注射后24小时或ATX-S10注射后2小时，通过YAGOPO激光器将移植的HeLa细胞肿瘤完全照射至50- 100 J/肿瘤，所述YAGOPO激光器产生对于Ph-II为630 nm波长的光和对于ATX-S10为670 nm波长的光。分别<31>于PDT后1、3、7天测量1 pMRS，并每天对标本进行组织病理学检查。ATX-S10光动力治疗后第1天，肿瘤组织中已检测不到<31>活细胞的~（1 p）MRS中ATP几乎消失，而活细胞仍存在的肿瘤组织中则有相当数量的ATP。在Ph-II PDT中，PDT后1天的ATP减少与根除的肿瘤体积无关，PDT后7天的总磷酸盐减少与活HeLa细胞减少相关。ATX-S10光动力治疗后第1天可见损伤细胞完全死亡，而Ph-LI治疗后细胞未完全死亡. 31 P MRS是评价光动力治疗效果和探讨其作用机制的一种非常有用的方法.为探讨光敏剂在实验性肝癌中的分布，用二乙基亚硝胺诱发大鼠肝癌11周。在该模型中观察到增生性结节和不典型结节等边界病变以及肝细胞癌。给药后1、2、3、4、6、8 h注射δ-氨基乙酰丙酸（ALA），用光诱导荧光检测系统检测原卟啉IX（PpIX）荧光。ALA给药后3 h，PpIX在肝癌组织中的荧光强度有增强的趋势，表明ALA可能在实验性肝癌组织中选择性蓄积。