The Evaluation of Photodynamic therapy (PDT) for cancer with ^<31>P nuclear magnetic resonance spectroscopy.

1.将光动力治疗(PDT)后测得的核磁共振波谱(~1t；31&gt；p MRS)与组织病理学结果进行对照分析。给BALB/c裸鼠腹腔注射PH-Il(Photofrin)或ATX-S10。注射Phil后24小时或注射ATX-S10后2小时，用波长分别为630 nm的Ph-II和670 nm的ATX-S 10的YAGOPO激光照射HeLa细胞移植瘤，照射剂量为50~100J/个肿瘤。在ATX-S10PDT后的第1天，肿瘤的31&gt；p MRS中的ATP几乎消失，其中未检测到活的HeLa细胞。相比之下，当活细胞被保留时，相当数量的ATP被识别出来。在Ph-II PDT中，PDT后1天的ATP下降与肿瘤体积无关，而PDT后7天的总磷下降与HeLa活细胞的减少有关。此外，我们还观察到ATX-S10 PDT后一天，受损的细胞已经完全死亡，而Ph-Li后的细胞并没有完全死亡。31P MRS是一种非常有用的评估PDT效果和探讨其机制的方法。为了研究光敏剂在实验性肝肿瘤中的分布，用二乙基亚硝胺诱发大鼠肝脏肿瘤11周。模型中可见增生性结节、不典型结节、肝细胞癌等交界性病变。于给药后1、2、3、4、6、8h注射D-氨基乙酰丙酸(ALA)，用光诱导荧光检测系统测定原卟啉IX(PpIX)的荧光强度。ALA给药后3h，癌组织中PpIX荧光强度较正常肝组织强，ALA在实验性肝癌中可能有选择性积聚。

1. The^<31>p nuclear magnetic resonance spectroscopy (^<31>P MRS ) measured after photodynamic therapy (PDT) were compared with the histopathological findings. Ph-Il (Photofrin) or ATX-S10 were intraperitoneally injected to BALB/c nude mice. 24 hours after Phil injection or two hours after the ATX-S10 injection the transplanted HeLa cell tumor was totally irradiated to 50-100J/tumor by YAGOPO laser which generates a light at a wavelength of 630 nm for Ph-II and 670 nm for ATX-S 10. The^<31>p MRS was measured 1, 3 and 7 days after PDT and the specimens were histopathologically studied each day. On the 1st day after ATX-S10 PDT, ATP almost disappeared in the^<31>p MRS of the tumor when live HeLa cells were undetectable in it. Contrastively, a considerable amount of ATP was recognized when live cells were remained. In Ph- II PDT, ATP decrease 1 day after PDT didn't correlate to the eradicated tumor volume, and the total phosphate decrease up to 7 days after PDT correlated to the decreasing live HeLa cells. Moreover, we observed one day after ATX- S10 PDT the damaged cells were completely dead, however the cells after Ph-LI were not comletely dead.31P MRS is a very useful method to evaluate the effects of PDT and to investigate its mechanisms.2. In order to investigate the distribution of photosensitizer in the experimental liver tumor, neoplastic hepatic lesions were induced in rats given diethylnitrosoamine for 11 weeks. Borderline lesions like hyperplastic nodule and atypical nodule, and hepatocellular carcinoma were observed in this model. delta -aminolevulinic acid (ALA) was injected and protoporphyrinIX (PpIX) fluorescence was measured using light induced fluorescence detection system 1,2,3,4,6,8 hours after administration. Three hours after ALA administration, PpIX fluorescence tended to be greater in cancer than in normal tissure.ALA might accumulate selectively in experimental liver cancer.