T cell immunity to biliary epithelial antigen in primary biliary cirrhosis.

原发性胆汁性肝硬化中，人胆道抗原p28可刺激外周血T淋巴细胞。通过尸检原发性胆汁性肝硬化患者的微细胞毒性实验也证实脾T淋巴细胞对自体胆道上皮细胞具有细胞毒性。HLAⅱ类基因DNA分型显示DRBl^<**>0803是与本病发生相关的HLAⅱ类基因之一。另一方面，HLA i类肿瘤坏死因子- α基因（tnf - α / Ncol RFLP, 5.5kb）和转运蛋白基因（Tap2/Ringll）与疾病无相关性。有趣的是，与DRBl^<**>0803阴性患者相比，p28对DRBl^<**>0803阳性患者T淋巴细胞的刺激更强烈。综上所述，p28可能是参与原发性胆汁性肝硬化发病机制的靶抗原之一。虽然p28的内部氨基酸序列尚未确定，但分析p28与t细胞受体和DRBl^<**>0803的相互作用对了解胆管损伤的机制具有重要意义。

In primary biliary cirrhosis, human biliary antigen p28 can stimulate peripheral blood T lymphocytes. It was also demonstrated that splenic T lymphocytes were cytotoxicic against autologous biliary epithelial cells by microcytotoxicity assay in autopsy patients with primary biliary cirrhosis.DNA typing of HLA class II genes showed that DRBl^<**>0803 is one of HLA class II genes related to the development of the disease. On the other hand, HLA class I.tumor necrosis factor-alpha genes(RFLP of TNF-alpha/ Ncol, 5.5kb) and transportor gene( Tap2/Ringll) are not associated with the disease.Interretingly, T Iymphocytes of DRBl^<**>0803 positive patients more strongly stimulated with p28, in comparison with DRBl^<**>0803 negative patients.In conclusion, p28 may be one of target antigens involved in the pathogenesis of primary biliary cirrhosis. Although inner amino asid sequense of p28 is not determined, analysis of interaction between p28, Tcell receptor and DRBl^<**>0803 is important in understanding the mechanism of biliary duct injury.