Modulation of thymocyte development by estrogen and autoimmune hepatitis

雌激素对胸腺细胞发育的调节和自身免疫性肝炎

• 批准号: 13670525
• 负责人: ONISHI Saburo
• 金额: $ 2.18万
• 依托单位: Kochi medical School
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2001
• 资助国家: 日本
• 起止时间: 2001 至 2002
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-13670525/
• 关键词: flowcytometry; aromatase; ArKO; CD25; CD44; estrogen; ArKO; thymocyte; auto immunity

Thymic regression and reduced cellularity were evident in aromatase-deficient mice. The major difficulties in thymocyte development were observed during the CD44^+CD25^- stage at the cortico-medullary junction and during the CD44^-CD25^- stage at the subcapsular region. The expression of Smoothened of the Hedgehog signaling was enhanced in CD4^-CD8^- double negative cells. This enhancement may result in impaired progression of CD44^-CD25^- cells to the CD4^+CD8^+ double positive stage and impaired proliferation of CD4^+CD8^+ double positive cells. This could be the reason why the proportion of CD3^+TCRβ^<high> cells during the late phase of thymocyte maturation was reduced in ArKO mice.

在芳香酶缺陷小鼠中，胸腺退化和细胞结构减少是明显的。胸腺细胞发育的主要困难发生在皮质髓质交界处的 CD44^+CD25^- 阶段和被膜下区域的 CD44^-CD25^- 阶段。 Hedgehog 信号的 Smoothened 表达在 CD4^-CD8^- 双阴性细胞中增强。这种增强可能导致 CD44^-CD25^- 细胞向 CD4^+CD8^+ 双阳性阶段的进展受损，并且 CD4^+CD8^+ 双阳性细胞的增殖受损。这可能是ArKO小鼠胸腺细胞成熟后期CD3^+TCRβ^<high>细胞比例减少的原因。

项目成果

C-L.Li, et al.: "Thymosin a1 accelerates restoration of T cell-mediated neutralizing antibody response in immunocompromised hosts."Intern Immunopharmacol. 2. 39-46 (2002)

C-L.Li 等人：“胸腺肽 a1 加速免疫受损宿主中 T 细胞介导的中和抗体反应的恢复。”Intern Nutrition

K.Toda, et al.: "Alterations in fatty-acid metabolism in the liver of ArKO male nice and their ameliorations by administration of 17 β -estradiol or a ligand of peroxisome proliferator-activated receptor α"J Steroid Biochem Mol Biol. 79. 11-17 (2002)

K. Toda 等人：“ArKO 雄性尼斯肝脏中脂肪酸代谢的改变及其通过施用 17 β-雌二醇或过氧化物酶体增殖物激活受体 α 配体的改善”J Steroid Biochem Mol Biol 79。 .11-17 (2002)

K.Toda, et al.: "Alterations in fatty-acid metabolism in the liver of ArKO male mice and their ameliorations by administration of 17 β-estradiol or a ligand of peroxisome proliferator-activated receptor α."J Steroid Biochem Mol Biol.. 79. 11-17 (2002)

K. Toda 等人：“ArKO 雄性小鼠肝脏中脂肪酸代谢的改变以及通过施用 17 β-雌二醇或过氧化物酶体增殖物激活受体 α 配体的改善。”J Steroid Biochem Mol Biol。 79. 11-17 (2002)

K.Toda, et al.: "Oestrogen at the neonatal stage is critical for the reproductive ability of male mice as revealed by supplementation with 17 β-estradiol to aromatase gene (Cyp19) knockout mice"J Endocrinol. 168. 455-463 (2001)

K. Toda 等人：“补充 17 β-雌二醇以芳香化酶基因 (Cyp19) 敲除小鼠表明，新生阶段的雌激素对于雄性小鼠的生殖能力至关重要”J Endocrinol. 168. 455-463 ( 2001）

Tsuda K, et al.: "Eosinophil-induced liver injury : an experimental model using IL-5 transgenic mice"J Hepatol.. 34. 270-277 (2001)

Tsuda K 等：“嗜酸性粒细胞诱导的肝损伤：使用 IL-5 转基因小鼠的实验模型”J Hepatol.. 34. 270-277 (2001)