Sigmficance of novel laminin in liver regeneration, acute injury and chronic fibrosis.

采用大鼠再生肝、急、慢性损伤肝及人缺血性肝、肝癌组织检测新型层粘连蛋白（laminin M）的定位及血清价值。层粘连蛋白M和层粘连蛋白A的结果不同，两种层粘连蛋白均局限于血管壁和胆管壁，但层粘连蛋白M也存在于门静脉区纤维组织中，急性损伤时层粘连蛋白M可局限于肝细胞等腺泡，慢性损伤时层粘连蛋白M可沿窦壁和增大的门静脉区纤维组织分布，而层粘连蛋白A仅存在于血管壁。纤层粘连蛋白M在肝纤维化组织中可见，而纤层粘连蛋白A在癌组织中可见。肝细胞癌患者血清层粘连蛋白A含量高，而急性和慢性肝炎患者血清层粘连蛋白M含量高，提示急性肝损伤和肝纤维化。在再生大鼠肝脏中，12小时早期在中央静脉周围的肝细胞中发现层粘连蛋白M，与急性损伤相似，层粘连蛋白A仅在肝壁中发现，层粘连蛋白通过细胞粘附分子在细胞-细胞或细胞-基质相互作用中发挥重要作用，层粘连蛋白M可能介导肝脏再生过程中的细胞-细胞相互作用和肝纤维化过程中的细胞-基质相互作用。

The localization and serum value of novel laminin(laminin M) were determined using regenerating liver, acute and chronic injured liver of rats and also human cimhotic liver, hepatocelleclar carcinoma. Different results between laminin M and laminin A were obtained, Both lamins were localized in vascular wall and bile duct wall, but laminin M was also found in fibrous tissue of portal area, laminin M was localized in acinus including hepatic cells during acute injury, along sinusoidal wall and fibrous tissue of enlarged porfal area during chronic injury, on the other hand, laminin A was only found in the vessel wall, In cases of liver cimhosis with heparocellular carcinoma, laminin M was recognized in fibrous tissue of cimhosis, on the contrary, laminin A was stained in carcinoma tissue. Serum laminin A was high in cases of hepatocllular carcinoma, However, laminin M was high in both cases of acute and chronic hepatitis, supporsed to indicate acute hepatic damage and also liver fibrosis.In the regenerating rat liver, laminin M was found in liver cells around central vein in early stage of 12 hours, which was similar to acute injury, laminin A was found only in sinusoidal wall, Laminin is supporsed to have important role in cell-cell or cell-matrix interaction by cell adhesion molecules Laminin M may mediate cell-cell interaction during liver regenaration and cell-matrix interaction during liver fibrosis.