Sigmficance of novel laminin in liver regeneration, acute injury and chronic fibrosis.

用大鼠再生肝、急、慢性肝损伤模型以及人肝、肝细胞癌模型，对新发现的层粘连蛋白(LN-M)进行了定位和血清学研究。层粘连蛋白M和层粘连蛋白A的结果不同，层粘连蛋白M和层粘连蛋白A均定位于血管壁和胆管壁，但层粘连蛋白M也存在于汇管区的纤维组织中，层粘连蛋白M在急性损伤时定位于包括肝细胞在内的腺泡内，在慢性损伤时沿肝窦壁和增大的门静脉区域的纤维组织定位，另一方面，层粘连蛋白A仅存在于血管壁，而在肝细胞癌合并肝纤维化的病例中，层粘连蛋白M定位于肝纤维化的纤维组织中，相反，层粘连蛋白A在癌组织中染色。肝细胞癌患者血清层粘连蛋白A水平较高，而急、慢性肝炎患者血清层粘连蛋白M水平均较高，提示急性肝损伤和肝纤维化。在再生大鼠肝组织中，层粘连蛋白M出现在肝中央静脉周围的肝细胞内，与急性肝损伤相似，层粘连蛋白A仅见于肝窦壁，层粘连蛋白通过细胞黏附分子在细胞-细胞或细胞-基质相互作用中发挥重要作用。层粘连蛋白M可能参与肝再生过程中的细胞-细胞相互作用和肝纤维化过程中的细胞-基质相互作用。

The localization and serum value of novel laminin(laminin M) were determined using regenerating liver, acute and chronic injured liver of rats and also human cimhotic liver, hepatocelleclar carcinoma. Different results between laminin M and laminin A were obtained, Both lamins were localized in vascular wall and bile duct wall, but laminin M was also found in fibrous tissue of portal area, laminin M was localized in acinus including hepatic cells during acute injury, along sinusoidal wall and fibrous tissue of enlarged porfal area during chronic injury, on the other hand, laminin A was only found in the vessel wall, In cases of liver cimhosis with heparocellular carcinoma, laminin M was recognized in fibrous tissue of cimhosis, on the contrary, laminin A was stained in carcinoma tissue. Serum laminin A was high in cases of hepatocllular carcinoma, However, laminin M was high in both cases of acute and chronic hepatitis, supporsed to indicate acute hepatic damage and also liver fibrosis.In the regenerating rat liver, laminin M was found in liver cells around central vein in early stage of 12 hours, which was similar to acute injury, laminin A was found only in sinusoidal wall, Laminin is supporsed to have important role in cell-cell or cell-matrix interaction by cell adhesion molecules Laminin M may mediate cell-cell interaction during liver regenaration and cell-matrix interaction during liver fibrosis.