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Sigmficance of novel laminin in liver regeneration, acute injury and chronic fibrosis.

新型层粘连蛋白在肝再生、急性损伤和慢性纤维化中的意义。

基本信息

批准号：
04670436
负责人：
SAKAI Takahiro
金额：
$ 1.34万
依托单位：
SAGA MEDICAL SCHOOL
依托单位国家：
日本
项目类别：
Grant-in-Aid for General Scientific Research (C)
财政年份：
1992
资助国家：
日本
起止时间：
1992 至 1993
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-04670436/
关键词：
Laminin Liver regeneration Liver fibrosis Liverdysfunction 細胞外マトリックス肝線維化

项目摘要

The localization and serum value of novel laminin(laminin M) were determined using regenerating liver, acute and chronic injured liver of rats and also human cimhotic liver, hepatocelleclar carcinoma. Different results between laminin M and laminin A were obtained, Both lamins were localized in vascular wall and bile duct wall, but laminin M was also found in fibrous tissue of portal area, laminin M was localized in acinus including hepatic cells during acute injury, along sinusoidal wall and fibrous tissue of enlarged porfal area during chronic injury, on the other hand, laminin A was only found in the vessel wall, In cases of liver cimhosis with heparocellular carcinoma, laminin M was recognized in fibrous tissue of cimhosis, on the contrary, laminin A was stained in carcinoma tissue. Serum laminin A was high in cases of hepatocllular carcinoma, However, laminin M was high in both cases of acute and chronic hepatitis, supporsed to indicate acute hepatic damage and also liver fibrosis.In the regenerating rat liver, laminin M was found in liver cells around central vein in early stage of 12 hours, which was similar to acute injury, laminin A was found only in sinusoidal wall, Laminin is supporsed to have important role in cell-cell or cell-matrix interaction by cell adhesion molecules Laminin M may mediate cell-cell interaction during liver regenaration and cell-matrix interaction during liver fibrosis.

用大鼠再生肝、急性和慢性损伤肝以及人肝癌、肝细胞癌等动物模型，测定了新层粘连蛋白（lamininM）的定位和血清学效价。层粘连蛋白M和层粘连蛋白A的定位结果不同，两者均定位于血管壁和胆管壁，但层粘连蛋白M也定位于汇管区纤维组织，层粘连蛋白M在急性损伤时定位于腺泡包括肝细胞，在慢性损伤时定位于沿着窦壁和扩大的汇管区纤维组织，而层粘连蛋白A仅定位于血管壁，肝纤维化伴肝细胞癌组织中层粘连蛋白A阳性，而肝纤维化伴肝细胞癌组织中层粘连蛋白M阳性。肝细胞癌患者血清层粘连蛋白A升高，而急性和慢性肝炎患者血清层粘连蛋白M均升高，提示急性肝损伤和肝纤维化，再生大鼠肝在12小时早期即可见层粘连蛋白M分布于中央静脉周围的肝细胞，与急性损伤相似，层粘连蛋白A仅分布于肝窦壁，层粘连蛋白M通过细胞粘附分子介导细胞-细胞或细胞-基质相互作用，在肝再生过程中介导细胞-细胞相互作用，在肝纤维化过程中介导细胞-基质相互作用。