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Regulation of gene expression of cholecystokinin in rat intestin e and mechanim or its release.

大鼠小肠胆囊收缩素基因表达调控及其释放机制。

基本信息

批准号：
04670450
负责人：
MIYASAKA Kyoko
金额：
$ 1.34万
依托单位：
Tokyo Metropolitan Institute of Gerontology
依托单位国家：
日本
项目类别：
Grant-in-Aid for General Scientific Research (C)
财政年份：
1992
资助国家：
日本
起止时间：
1992 至 1993
项目状态：
已结题

项目摘要

1. We established the assay system of mRNA levels of cholecystokinin (CCK) and secretin, using the TR-PCR products of 343 bp cDNA (the sense primer 5'-AGCCGGTAGTCCCTGTAGAA-3' and anti-sense primer 5'-GTGCGTGGTTGTTTTCTCAT-3') for CCK and 426 bp cDNA 9the sense primer 5'ATGGAGCCTCTACTGCCCACGC-3' and anti-sense primer 5'-AGGGAGGAAGGGAGTCTCCAG-3') for secretin, respectively.2. Exclusion of bile and pancreatic juice significantly increased plasma CCK concentrations, peaking at 1-2 h after diversion. Excludion of bile and pancreatic jucie also enhanced gene expressions of both CCK and secretin, peaking at 4 h after diversion.3. The concentrations of plasma and intestinal CCK, and the levels of mRNA of CCK in the intestinal mucosa and PSTIs in the pancreas were significantly increased by the bile-pancreatic juice diversion. The increase in the level of mRNA for PSTI-61 was igher than that for PSTI-56. CCK-antagonist inhibited these changes but administration of a CCK agonist could not fully reproduce them.4. The CCK mRNA level initially decreased on day 1 and then increased and peaked on day 10, slightly decreased on day 14 and returned to the control level on day 28 after ancreatic duct lgaton. The level of mRNA of PSTIs increased significantly on days 1, 3, 7, 10 after occlusion. The mRNA level of PAP appeared on days 1 and 3, and the maximal increase was observed on day 1. The changes of gene expression of PAP reflects the acute inflammatory changes of ancreas most sensitively.

1. 利用CCK 343 bp cDNA（正义引物5'-AGCCGGTAGTCCCTGTAGAA-3'和反义引物5'-GTGCGTGGTTGTTTTTCTCAT-3'）和426 bp cDNA 9正义引物的TR-PCR产物，建立了胆囊收缩素（CCK）和促胰液素mRNA水平的检测体系。分别为促胰液素的5'ATGGAGCCTCTACTGCCCACGC-3'和反义引物5'-AGGGAGGAAGGGGAGTCTCCAG-3')。2．排除胆汁和胰液显着增加血浆 CCK 浓度，在转移后 1-2 小时达到峰值。排除胆汁和胰液也增强了CCK和促胰液素的基因表达，在转移后4小时达到峰值。3．胆胰液分流显着增加了血浆和肠道CCK浓度、肠粘膜CCK mRNA水平和胰腺PSTIs水平。 PSTI-61 mRNA水平的增加高于PSTI-56。 CCK拮抗剂抑制了这些变化，但施用CCK激动剂不能完全重现这些变化。4． CCK mRNA水平在第1天开始下降，然后增加并在第10天达到峰值，在第14天略有下降，并在胰管结扎后第28天恢复到对照水平。 PSTIs mRNA水平在闭塞后第1、3、7、10天显着增加。 PAP mRNA水平在第1天和第3天出现，并在第1天出现最大增加。PAP基因表达的变化最敏感地反映了胰腺的急性炎症变化。