EFFECT OF INTRACAROTIDLY INFUSED PLATELET-ACTIVATING FACTOR ON FELINE CEREBROCORTICAL BLOOD FLOW

颈动脉内注入血小板激活因子对猫脑皮质血流的影响

• 批准号: 04670496
• 负责人: TANAHASHI Norio
• 金额: $ 1.41万
• 依托单位: KEIO University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for General Scientific Research (C)
• 财政年份: 1992
• 资助国家: 日本
• 起止时间: 1992 至 1993
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-04670496/
• 关键词: Platelet-activating factor(PAF); cerebral blood flow(CBF); photoelectric method; cerebral blood volume(CBV); cerebral cortex; systemic arterial blood pressure(SABP); veactive hyperemia; mean transit time; 脳循環; 血小板活性化因子

An increasing number of reports have suggested the possible involvement of the platelet-activating factor (PAF) in processes associated with cerebral ischemia and neurotrauma. The purpose of the present study was to examine the effect of intracarotidly infused PAF on the cerebrocortical blood flow.Materials and Method : Seven cats were anesthetized and artificially ventilated. The left femoral and lingual arteries were cannulated. They were used to monitor the systemic arterial blood pressure (SABP) and to inject carbon-black (CB) solution into the carotid artery, respectively. A photoelectric apparatus (Am J Physiol 235 : H56-H63, 1978) was attached to the left parietal cortex, and the cerebral blood volume (CBV) and mean transit time of blood through the cortical tissue (MTT) were measured. The cerebral blood flow (CBF) was calculated form the CBV and the MTT of blood by applying the Stewart-Hamilton equation and cerebrocortical vascular resistance (CVR) was further calculated using … More the formula, mean arterial blood pressure (MABP)/CBF.PAF (0.01g/kg) suspended in 0.25% bovine serum albumin in 1 ml of saline was infused into the left carotid artery within 15s. Result : Immediately after the PAF administration, the mean arterial blood pressure (MABP) decreased form 125(〕SY.+-.〔)20 to 107(〕SY.+-.〔)18 mmHg (p<0.05) and returned to the previous level in 5 min (130(〕SY.+-.〔)17mm Hg). CBV remained constant throughout ; i.e., before (6.3 vol%), immediately after (6.0(〕SY.+-.〔)0.3%), and 5 min after the administration (6.4(〕SY.+-.〔)0.5 vol%). MTT was increased immediately after the PAF administration from 5.9(〕SY.+-.〔)2.1 to 8.3(〕SY.+-.〔)3.4 s (p<0.05) and returned to the previous level in 5 min (5.6(〕SY.+-.〔)1.9 s). CBF was decreased immediately after the PAF administration from 73(〕SY.+-.〔)29 to 51(〕SY.+-.〔)21 ml/100g brain/min (p<0.01) and returned to the previous level in 5 min (76(〕SY.+-.〔)24 ml/100g brain/min). CVR was increased immediately after the PAF administration from 1.92(〕SY.+-.〔)0.47 to 2.36(〕SY.+-.〔)0.74 mmHg/ml/100g brain/min (p<0.05) and returned to the previous level in 5 min (1.92(〕SY.+-.〔)0.43 mmHg/ml/100g brain/min). Conclusion : Intracarotidly infused PAF momentarily decreased the cerebrocortical blood flow. Less

越来越多的研究表明血小板活化因子（PAF）可能参与脑缺血和神经损伤的过程。本研究的目的是研究PAF对大脑皮层血流的影响。材料和方法：7只猫麻醉后，人工通气。对左股动脉和舌动脉插管。分别用于监测体循环动脉血压（SABP）和颈动脉内注射炭黑（CB）溶液。将光电装置（Am J Physiol 235：H56-H63，1978）连接到左顶叶皮层，并测量脑血容量（CBV）和血液通过皮层组织的平均通过时间（MTT）。应用Stewart-Hamilton公式从CBV和MTT计算脑血流量（CBF），并进一步计算皮质血管阻力（CVR）。 ...更多信息 将PAF（0.01g/kg）混悬于0.25%牛血清白蛋白的生理盐水1 ml中，于15 s内注入左颈动脉。结果：PAF治疗后即刻，平均动脉压（MABP）由治疗前的125 SY ± 0.5mmHg降至治疗前的125 SY ± 0.5mmHg; ()20至107（）SY ±。()18 mmHg（P<0.05），5 min后恢复到前一水平（130 ± 0.05）mmHg。（）17 mm Hg）。CBV始终保持恒定;即，前（6.3体积%），后立即（6.0（）SY. ±. ()0.3给药后5 min（6.4（± 0.05）SY. ()0.5体积%）。在PAF给药后，MTT立即从5.9（）SY. ±. ()2.1至8.3（）SY. ±. ()3.4 s（p<0.05），5 min后恢复到原来水平（5.6 ± 0.05）。()1.9 s）。灌胃PAF后即刻，CBF从73 ± 0.05下降到73 ± 0.05，与对照组比较差异有统计学意义（P < 0.05）。()29至51（）SY. ()21 ml/100 g脑/min（P<0.01），5 min后恢复到原来水平（76 ± 0.05）。()24 ml/100 g脑/min）。CVR在PAF给药后即刻由1.92 ± 0.05增加到1.92 ± 0.05。（）0.47 ~ 2.36（）SY ±.（）0.74mmHg/ml/100 g脑/min（p<0.05），5 min后恢复到原来水平（1.92 ± 0.05）。（）0.43 mmHg/ml/100 g脑/min）。结论：PAF可使脑皮质血流量短暂下降。少

项目成果

N.Tanahashi,et al: "Effect of intracarotidly infusecl platelet-activating factor on feline cerebrocortical flow resitance" International Journal of Microcirculation clinical and Experimental. 11(sappl1). S62- (1992)

N.Tanahashi 等人：“颈动脉内注射血小板激活因子对猫脑皮质血流阻力的影响”《国际微循环临床与实验杂志》。