Molecular mechanism of activated platelet adhesion to human brain microvascular endothelial cells under flow in vitro
体外流动条件下活化血小板粘附人脑微血管内皮细胞的分子机制
基本信息
- 批准号:14570616
- 负责人:
- 金额:$ 2.24万
- 依托单位:
- 依托单位国家:日本
- 项目类别:Grant-in-Aid for Scientific Research (C)
- 财政年份:2002
- 资助国家:日本
- 起止时间:2002 至 2003
- 项目状态:已结题
- 来源:
- 关键词:
项目摘要
We previously reported that activated platelets adhered to human brain microvascular endothelial cells (HBEC) under flow in vitro using video enhanced contrast (VEC) microscopy. The purpose of the present study was to examine the effect of Arg-Gly-Asp-Ser (RGDS) peptide which block the binding of von willebrand factor (vWF), fibrinogen, and fibronectin to GPIIb/IIIa and NMC-4 which block the binding of vWF to GPIb on platelet adhesion to HBEC.Methods : To evaluate platelet adhesion at high magnification, we employed a video-enhanced contrast (VEC) microscopy system. in the control group (N=6): HBEC's were cultured on a coverglass and put in the observation chamber of VEC microscopy. PRP was superfused with an infusion pump at a low shear rate (10/sec) for 30 ruin and washed out. Interaction between platelets and endothelial cells was observed by VEC-DIC microscopy and the number of platelets adhered to endothelial cells was calculated. In the ADP group (N=7); PRP containing ADP (2μM) w … More as superfused on HBEC for 30 min and washed out. In the RGDS group (N=6): PRP containing ADP(2μm) and RGDS peptide (50μg/ml) was superfused on HBEC for 30 min and washed out, in the NMC-4 group (N=6): PRP containing ADP (2μM) and NMC4 (10μg/ml) was superfused for 30 min and washed out.Results : In the control group, platelet adhesion to HBEC was rarely seen, in the ADP group, platelets adhered to HBEC and microaggregates of platelets were seen. In the RGDS group, platelet adhesion to HBEC was rarely seen. In the NMG4 group, platelets adhered to HBEC and microaggregates of platelets were seen. The average number of platelets adhering acid aggregating to endothelial cells was 0.3 ± 0.7/900μm^2 in the control group, 22.3 ± 10.4/900μm^2 in the ADP group (P<0.01, vs control group), 0.3 ± 0.2/900μm^2 in the RGDS group (p<0.01, vs ADP goup) and 18.0 ± 11.6/900μm^2 in the NMC-4 group.Conclusion : The present study showed RGDS peptide, but not NMG4, ameliorated platelet adhesion to HBEC at a low-flow state in vitro This suggests that the binding of vWF, fibrinogen, and fibronectin to GPIIb/IIIa plays an important role in platelet adhesion to HBEC. Less
我们以前报道过,激活血小板粘附人脑微血管内皮细胞(HBEC)在体外流动下,使用视频增强对比(VEC)显微镜。本研究的目的是检查Arg-Gly-Asp-Ser(RGDS)肽阻断血管性血友病因子(vWF),纤维蛋白原,和纤连蛋白GPIIb/IIIa和NMC-4阻断vWF GPIb对血小板粘附HBEC.Methods的结合的影响:为了评估血小板粘附在高放大倍数,我们采用了视频增强对比(VEC)显微镜系统。对照组(N=6):HBEC在盖玻片上培养,并置于VEC显微镜观察室中。用输注泵以低剪切速率(10/sec)灌注PRP 30分钟并冲洗。采用VEC-DIC显微镜观察血小板与内皮细胞的相互作用,计算血小板粘附内皮细胞的数量。ADP组(N=7):PRP含ADP(2μM), ...更多信息 在HBEC上灌流30分钟并洗出。RGDS组(N=6):将含ADP(2μM)和RGDS肽(50μg/ml)的PRP在HBEC上灌流30 min后洗出,NMC-4组(N=6):将含ADP(2μM)和NMC-4(10μg/ml)的PRP在HBEC上灌流30 min后洗出。对照组血小板与HBEC粘附极少,ADP组血小板与HBEC粘附,可见血小板微聚集。在RGDS组中,血小板粘附于HBEC很少见到。在NMG 4组中,血小板粘附于HBEC,并观察到血小板微聚集体。对照组粘附于内皮细胞的血小板平均数为0.3 ± 0.7/900μm^2,ADP组为22.3 ± 10.4/900μm^2 RGDS组为0.3 ± 0.2/900μm^2(P<0.01)NMC-4组为18.0 ± 11.6/900μm^2,与ADP组相比,P<0.01。本研究表明,RGDS肽,而不是NMG 4,在体外改善了血小板在低流量状态下与HBEC的粘附。这表明vWF,纤维蛋白原,而纤连蛋白与GPIIb/IIIa的结合在血小板与HBEC的粘附中起重要作用。少
项目成果
期刊论文数量(6)
专著数量(0)
科研奖励数量(0)
会议论文数量(0)
专利数量(0)
Tanahashi N et al.: "Molecular mechanism of adenosine diphosphate- activated platelets adhesion to human brain microvascular endothelial cells under flow in vitro"Microcirculation annual. 19. 13-14 (2003)
Tanahashi N等人:“二磷酸腺苷激活血小板在体外流动下粘附人脑微血管内皮细胞的分子机制”微循环年度。
- DOI:
- 发表时间:
- 期刊:
- 影响因子:0
- 作者:
- 通讯作者:
Tanahashi N et al.: "Molecular mechanism of adenosine diphosphate-activated platelet adhesion to human brain microvascular endothelial cells under flow in vitro"Microcirculation annual. 19. 13-14 (2003)
Tanahashi N等人:“二磷酸腺苷激活血小板在体外流动下粘附人脑微血管内皮细胞的分子机制”微循环年刊。
- DOI:
- 发表时间:
- 期刊:
- 影响因子:0
- 作者:
- 通讯作者:
Tanahashi N, Itoh Y, et al.: "Molecular mechanism of adenosine diphosphate-activated platelet adhesion to human brain microvascular endothelial cells."Microcirculation annual. 19. 13-14 (2003)
Tanahashi N、Itoh Y 等人:“二磷酸腺苷激活血小板粘附人脑微血管内皮细胞的分子机制。”微循环年度。
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- 影响因子:0
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TANAHASHI Norio其他文献
TANAHASHI Norio的其他文献
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{{ truncateString('TANAHASHI Norio', 18)}}的其他基金
Platelet adhesion to human brain microvascular endothelial cells in vitro
血小板对人脑微血管内皮细胞的体外粘附
- 批准号:
12670617 - 财政年份:2000
- 资助金额:
$ 2.24万 - 项目类别:
Grant-in-Aid for Scientific Research (C)
Platelet adhesion to endothelial cells in vitro, observation with video-enhanced contrast (VEC) microscopy
体外血小板与内皮细胞的粘附,用视频增强对比 (VEC) 显微镜观察
- 批准号:
10670601 - 财政年份:1998
- 资助金额:
$ 2.24万 - 项目类别:
Grant-in-Aid for Scientific Research (C)
Rogulatory mechanism of Cerebral microcirculation
脑微循环的调节机制
- 批准号:
08670724 - 财政年份:1996
- 资助金额:
$ 2.24万 - 项目类别:
Grant-in-Aid for Scientific Research (C)
EFFECT OF INTRACAROTIDLY INFUSED PLATELET-ACTIVATING FACTOR ON FELINE CEREBROCORTICAL BLOOD FLOW
颈动脉内注入血小板激活因子对猫脑皮质血流的影响
- 批准号:
04670496 - 财政年份:1992
- 资助金额:
$ 2.24万 - 项目类别:
Grant-in-Aid for General Scientific Research (C)
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