Effect of EDLF on Ca^<2+>, Na^+ pump in cultured vascular smooth muscle cells

近年来对内皮细胞与血管平滑肌细胞（VSMC）相互作用的研究揭示了内皮细胞与VSMC之间的相互调节机制，其中包括内皮素（ET）、内皮素释放因子（EDRF）和血管紧张素Ⅱ（Escherichia coli，Escherichia coli，Escherichia coli，Escherichia coli）等体液调节因子。为了进一步研究内皮源性激素（endothelium-derived hormone，ET-1）对血管平滑肌细胞阳离子转运的影响，我们尝试了一种新的培养体系--血管平滑肌细胞与内皮细胞共培养。第一部分研究表明，ET-1可促进Na-K泵活性、Na-H逆向转运和Na-K共转运。ET-1本身也能刺激VSMC胞内Ca内流，使细胞内游离Ca浓度增加，本研究的第二部分是用硝基药物模拟EDRF的作用，观察了硝基药物对VSMC阳离子转运系统的影响，结果表明NO对VSMC的增殖有抑制作用。共培养实验无法在研究的2年期限内完成，因此在没有结果的情况下终止。

Recent investigations of the interaction between endothelial cells and vascular smooth muscle clls(VSMC) have disclosed mutual regulatory mechanism, utilizing humoral factors, between the two cells.Such regulatory humoral factors include endothelin, EDRF and prostaglandins.Present study has, in this context, focused on the interrelationship between the two cells. To pursue this, I assessed the effect of endothlium-derived hormone(ET-1) on cation transport of VSMC.In addition, I attempted a new culture system, co-culture of VSMC and endothelial cells. The 1st part of the study showed that ET-1 stimulates Na-K pump activity, Na-H antiport and Na-K contransport. ET-1, per se, also stimulates Ca influx, resulting in an increase in cytosolic free Ca concentration of VSMC.Knowing that a nitro-based agent may mimic the effect of EDRF, the 2nd part of this study described the effect of nitro-based pharmacological agent on cation transport s stems of VSMC, which showed an inhibitory action of NO on the proliferation of VSMC.Because of time-c nsuming technical difficulties, the co-culture experiment could not be completed within the study's 2-year limit and thus was terminated without results.