Effect of EDLF on Ca^<2+>, Na^+ pump in cultured vascular smooth muscle cells

EDLF对培养血管平滑肌细胞Ca^2、Na^泵的影响

• 批准号: 04670558
• 负责人: KURIYAMA Satoru
• 金额: $ 1.22万
• 依托单位: Jikei University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for General Scientific Research (C)
• 财政年份: 1992
• 资助国家: 日本
• 起止时间: 1992 至 1993
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-04670558/
• 关键词: Na, Ca-dump; Vascular Smooth Muscle Cells; Endothelial cells; Eudothelin; EDLF; culture; Hypertension; Na,Ca‐pump; EDRF

Recent investigations of the interaction between endothelial cells and vascular smooth muscle clls(VSMC) have disclosed mutual regulatory mechanism, utilizing humoral factors, between the two cells.Such regulatory humoral factors include endothelin, EDRF and prostaglandins.Present study has, in this context, focused on the interrelationship between the two cells. To pursue this, I assessed the effect of endothlium-derived hormone(ET-1) on cation transport of VSMC.In addition, I attempted a new culture system, co-culture of VSMC and endothelial cells. The 1st part of the study showed that ET-1 stimulates Na-K pump activity, Na-H antiport and Na-K contransport. ET-1, per se, also stimulates Ca influx, resulting in an increase in cytosolic free Ca concentration of VSMC.Knowing that a nitro-based agent may mimic the effect of EDRF, the 2nd part of this study described the effect of nitro-based pharmacological agent on cation transport s stems of VSMC, which showed an inhibitory action of NO on the proliferation of VSMC.Because of time-c nsuming technical difficulties, the co-culture experiment could not be completed within the study's 2-year limit and thus was terminated without results.

近年来对内皮细胞与血管平滑肌细胞（VSMC）相互作用的研究揭示了内皮细胞与VSMC之间的相互调节机制，其中包括内皮素（ET）、内皮素释放因子（EDRF）和血管紧张素Ⅱ（Escherichia coli，Escherichia coli，Escherichia coli，Escherichia coli）等体液调节因子。为了进一步研究内皮源性激素（endothelium-derived hormone，ET-1）对血管平滑肌细胞阳离子转运的影响，我们尝试了一种新的培养体系--血管平滑肌细胞与内皮细胞共培养。第一部分研究表明，ET-1可促进Na-K泵活性、Na-H逆向转运和Na-K共转运。ET-1本身也能刺激VSMC胞内Ca内流，使细胞内游离Ca浓度增加，本研究的第二部分是用硝基药物模拟EDRF的作用，观察了硝基药物对VSMC阳离子转运系统的影响，结果表明NO对VSMC的增殖有抑制作用。共培养实验无法在研究的2年期限内完成，因此在没有结果的情况下终止。

项目成果

KURIYAMA S et al.: "Lowered Insulin sensitive Ca^<2+> transport in cultured glomerular mesangial cells from SHR" Jpn J Nephrol. 35(8). 893-899 (1993)

KURIYAMA S等人：“降低来自SHR的培养的肾小球系膜细胞中的胰岛素敏感性Ca 2+ 转运”Jpn J Nephrol。

"動脈中膜平滑筋細胞増殖能に及ぼすβ遮断剤、ニプラジロールの抑制作用" 動脈硬化. 20(12). 999-1004 (1993)

“β-受体阻滞剂尼普地洛对动脉中膜平滑肌细胞增殖的抑制作用”动脉硬化20(12)。

"血管平滑筋細胞細胞膜Ca2+輸送系に対する液性因子の研究" Ther Res. 14(1). 66-71 (1993)

“体液因素对血管平滑肌细胞膜Ca2+转运系统的研究”Ther Res 14(1) (1993)。

"Lowered Insulin sensitive Ca2+ transport in cultured glomerular mesangial cells from SHR" Jpn J Nephrol. 35(8). 893-899 (1993)

“SHR 培养的肾小球系膜细胞中胰岛素敏感性 Ca2 转运降低”Jpn J Nephrol。

栗山哲他: "動脈中膜平滑筋細胞増殖能に及ぼすbeta遮断剤、ニプラジロールの抑制作用" 動脈硬化. 20(12). 999-100〓 (1993)

Satoshi Kuriyama 等人：“β 受体阻滞剂尼普地罗对动脉中膜平滑肌细胞增殖的抑制作用”，Arteriosclerosis 20(12)。