Abnormal Ca and Na pump of cultured vascular smooth muscle cells from hypostensive rats.

低血压大鼠培养血管平滑肌细胞的钙泵和钠泵异常。

• 批准号: 02670410
• 负责人: KURIYAMA Satoru
• 金额: $ 1.28万
• 依托单位: Jikei University School of Medicine
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for General Scientific Research (C)
• 财政年份: 1990
• 资助国家: 日本
• 起止时间: 1990 至 1991
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-02670410/
• 关键词: Ca pump; Na pump; SHR; Hypertension; Vascular smooth muscle cells; culture; vascular smooth musde cells; 血管平滑筋細胞膜; NaーK pump; Caーpump; NaーK contransport; NaーH antiport; 増殖

To elineate abnormalities in membrane permeability to cations as well as differential characteristic of humoral factors in the etiology of hypertension, Ca and Na transpport kinetics of vascular smooth muscle cells and the effect of serum from spontaneously hypertensive rats (SHR) on these transports were addressed using culture cells.A substantial activation in both Ca and Na pump was observed in VSMC of SHR compared to that of WKY. This difference may be caused by an increased Na-H antiport of SHR VSMC. In the presence of serum from SHR, there was a greater stimulation of Na-pump, which was originally triggered by the actiation of Na-H antiport.These evidences strongly suggest not only an abnormally increased membrane permeability of SHR cell membrane which is innate to SHR cells but also the involvement of some unknown humoral factors which elicits an activation of Na-pump followed by an activation of NA-H antiport.

为了消除细胞膜对阳离子的渗透性异常以及高血压病因中体液因子的差异特征，我们利用培养细胞研究了血管平滑肌细胞钙钠转运动力学以及自发性高血压大鼠血清对这些转运的影响。与WKY相比，在SHR的VSMC中观察到Ca和Na泵的大量激活。这种差异可能是由SHR VSMC的Na-H反端口增加引起的。在SHR血清存在的情况下，na泵的刺激更大，这最初是由Na-H反港的激活引发的。这些证据有力地表明，SHR细胞膜的通透性异常增加不仅是SHR细胞固有的，而且还涉及一些未知的体液因子，这些因子引起na泵的激活，然后是NA-H反端口的激活。

项目成果

Kuriyama S et al.: "Increased Na-K transport in gomerular mesangial cells from SHR." Nephron. (1992)

Kuriyama S 等人：“SHR 增加了戈小球系膜细胞的 Na-K 转运。”

Kuriyama S et al.: "A greater stimulation of vascular smooth muscle cells by serum from SHR." Acta Cardiol. 47. (1992)

Kuriyama S 等人：“SHR 血清对血管平滑肌细胞有更大的刺激。”

Kuriyama S et al.: "A greater stimulation of Na-H antiport in cultured vascular smooth muscle cells by serum from SHR." Am J Hypertens. 4. 780-782 (1991)

Kuriyama S 等人：“SHR 血清对培养的血管平滑肌细胞中 Na-H 反向转运有更大的刺激。”

Kuriyama S et al.: "A greater stimulation of vascualr smooth muscle cells by serum from SHR." Acta Cardiol. 47. (1992)

Kuriyama S 等人：“SHR 血清对血管平滑肌细胞有更大的刺激。”

Kuriyama S et al: "Effect of serum from spontaneously hypertensive rats on 86Rb washout from culured vascular smooth muscle cells." Acta Cardiol. 65. 35-43 (1990)

Kuriyama S 等人：“自发性高血压大鼠血清对培养血管平滑肌细胞 86Rb 洗脱的影响。”