Basic and clinical studies of the distribution of radioactive metal elements in the maliganant tumor by autoradiography

本研究旨在用放射自显影法观察放射性金属元素在恶性肿瘤组织中的分布，探讨肿瘤组织中的蓄积与这些金属元素性质的关系。此外，本研究旨在研究炎症病变中1Ga积累的机制<67>。在埃利希瘤和吉田肉瘤中，分别用~（60）<67>Ga-柠檬酸盐和~<111>（60）In-柠檬酸盐处理，这些核素的浓度主要集中在炎性组织中，而不是活的肿瘤组织中，在坏死的肿瘤组织中几乎不存在。在埃利希瘤和吉田肉瘤中，分别用~（60）<201>Tl-氯化物<86>、~（60）Rb-氯化物和<134>~（60）Cs-氯化物处理，这些核素大量积聚在活的肿瘤组织中，只有少量积聚在结缔组织中。无论给药后的时间如何，并且在坏死的肿瘤组织中未观察到。在用氯化钠注射肿瘤的情况<22>下，<22>无论注射后的时间如何，在坏死的肿瘤组织中的氯化钠浓度比在存活的肿瘤组织和结缔组织中的氯化钠浓度更占优势。根据我们的实验数据，<67>可以得出如下结论：炎症病变中的Ga摄取机制如下：<67>Ga在炎症病变中的积聚主要是由于<67>Ga通过具有增加的渗透性的毛细血管渗漏到浸润有中性粒细胞和巨噬细胞的皮下组织中。在炎性病变中，<67>Ga优先与组成细胞间物质的酸性粘多糖结合。

The purpose of this study is to observe thedistribution of the radioactive metal elements in malignant tumor tissue by autoradiography and to invesigate the relationship between the accumulation in tumor tissue and properties of these metal elements. In addition, this study is undertaken to investigate the mechanism of accumulation of ^<67> Ga in the inflammatory lesion. In Ehrlich tumor and Yoshida sarcoma which had been administered with ^<67>Ga-citrate and ^<111>In-citrate, respectively, the concentrations of these nuclides were predominant in inflammatory tissues rather than in viable tumor tissue, and were not seem in necrotic tumor tissue.In the case of Ehrlich tumor and Yoshida sarcoma administered with ^<201>Tl-chloride, ^<86>Rb-chloride and ^<134>Cs-chloride, respectively, a large amount of these nuclides was accumulated in viable tumor tissue, andonly small amounts were accumulated in connective tissue, regardless of the time after administration, and were not seen in necrotic tumor tissue. In the case of tumors injucted with ^<22>Na-chloride, the concentration of ^<22>Na was more dominant in necrotic tumor tissue than in viable tumor tisue and connective tissue, regardless of the time after injuctions..From data of our experiments, mechanisms of ^<67>Ga uptake into inflammatory lesion were concluded as follows : accumulation of _<67>Ga in the inflammatory lesion is primarily due to leakage of _<67>Ga into the subcutaneo tissue infiltrated with neutrophils and macrophages through capillaries with increased permeability. In the inflammatory lesion, _<67>Ga is preferentially bound to the acid mucopolysaccharides which compose intercellular substances.