Basic and clinical studies of the distribution of radioactive metal elements in the maliganant tumor by autoradiography

本研究的目的是用放射自显影技术观察放射性金属元素在恶性肿瘤组织中的分布，并探讨放射性金属元素在肿瘤组织中的蓄积与其性质的关系。此外，本研究还探讨了炎性病变中^&lt；67&gt；Ga蓄积的机制。在Ehrlich肿瘤和Yoshida肉瘤中，分别用柠檬酸镓和柠檬酸注射的艾利希肿瘤和吉田肉瘤中，这些核素的浓度主要分布在炎症组织中，而不是在活体肿瘤组织中，并且在坏死性肿瘤组织中没有出现。CS-Cl2分别在活体肿瘤组织中大量积聚，在结缔组织中仅有少量积聚，在坏死性肿瘤组织中未见分布。在Na-Na损伤的肿瘤中，无论何时，坏死性肿瘤组织中~&lt；22&gt；Na的浓度均高于存活的肿瘤组织和结缔组织。根据我们的实验数据，炎性病变摄取~&lt；67&gt；Ga的机制如下：炎性病变中~&lt；67&gt；Ga的蓄积主要是由于~&lt；67&gt；Ga通过毛细血管和巨噬细胞渗入皮下组织，通透性增加所致。在炎性病变中，_&lt；67&gt；GA优先与组成细胞间物质的酸性粘多糖结合。

The purpose of this study is to observe thedistribution of the radioactive metal elements in malignant tumor tissue by autoradiography and to invesigate the relationship between the accumulation in tumor tissue and properties of these metal elements. In addition, this study is undertaken to investigate the mechanism of accumulation of ^<67> Ga in the inflammatory lesion. In Ehrlich tumor and Yoshida sarcoma which had been administered with ^<67>Ga-citrate and ^<111>In-citrate, respectively, the concentrations of these nuclides were predominant in inflammatory tissues rather than in viable tumor tissue, and were not seem in necrotic tumor tissue.In the case of Ehrlich tumor and Yoshida sarcoma administered with ^<201>Tl-chloride, ^<86>Rb-chloride and ^<134>Cs-chloride, respectively, a large amount of these nuclides was accumulated in viable tumor tissue, andonly small amounts were accumulated in connective tissue, regardless of the time after administration, and were not seen in necrotic tumor tissue. In the case of tumors injucted with ^<22>Na-chloride, the concentration of ^<22>Na was more dominant in necrotic tumor tissue than in viable tumor tisue and connective tissue, regardless of the time after injuctions..From data of our experiments, mechanisms of ^<67>Ga uptake into inflammatory lesion were concluded as follows : accumulation of _<67>Ga in the inflammatory lesion is primarily due to leakage of _<67>Ga into the subcutaneo tissue infiltrated with neutrophils and macrophages through capillaries with increased permeability. In the inflammatory lesion, _<67>Ga is preferentially bound to the acid mucopolysaccharides which compose intercellular substances.