Basic and clinical studies of the distribution of radioactive metal elements in the maliganant tumor by autoradiography

放射自显影放射性金属元素在恶性肿瘤中分布的基础与临床研究

• 批准号: 04670663
• 负责人: ANDO Atsushi
• 金额: $ 1.34万
• 依托单位: Kanazawa University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for General Scientific Research (C)
• 财政年份: 1992
• 资助国家: 日本
• 起止时间: 1992 至 1993
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-04670663/
• 关键词: Malignant tumor; Radioactive metal elements; Ga-67; Tl-201; Autoradiography; Inflammatory lasion; 炎症巣; 放射性金属元素; インジウム-111; アルカリ金属; 組識内分布

The purpose of this study is to observe thedistribution of the radioactive metal elements in malignant tumor tissue by autoradiography and to invesigate the relationship between the accumulation in tumor tissue and properties of these metal elements. In addition, this study is undertaken to investigate the mechanism of accumulation of ^<67> Ga in the inflammatory lesion. In Ehrlich tumor and Yoshida sarcoma which had been administered with ^<67>Ga-citrate and ^<111>In-citrate, respectively, the concentrations of these nuclides were predominant in inflammatory tissues rather than in viable tumor tissue, and were not seem in necrotic tumor tissue.In the case of Ehrlich tumor and Yoshida sarcoma administered with ^<201>Tl-chloride, ^<86>Rb-chloride and ^<134>Cs-chloride, respectively, a large amount of these nuclides was accumulated in viable tumor tissue, andonly small amounts were accumulated in connective tissue, regardless of the time after administration, and were not seen in necrotic tumor tissue. In the case of tumors injucted with ^<22>Na-chloride, the concentration of ^<22>Na was more dominant in necrotic tumor tissue than in viable tumor tisue and connective tissue, regardless of the time after injuctions..From data of our experiments, mechanisms of ^<67>Ga uptake into inflammatory lesion were concluded as follows : accumulation of _<67>Ga in the inflammatory lesion is primarily due to leakage of _<67>Ga into the subcutaneo tissue infiltrated with neutrophils and macrophages through capillaries with increased permeability. In the inflammatory lesion, _<67>Ga is preferentially bound to the acid mucopolysaccharides which compose intercellular substances.

本研究的目的是利用放射自显影技术观察恶性肿瘤组织中放射性金属元素的分布，探讨放射性金属元素在肿瘤组织中的富集与这些金属元素的性质之间的关系。此外，本研究旨在探讨^<67> Ga在炎性病变中的积累机制。在Ehrlich肿瘤和吉田肉瘤中，分别给予^<67> ga -柠檬酸盐和^<111>In-柠檬酸盐，这些核素的浓度主要在炎症组织而不是活的肿瘤组织中，并且在坏死的肿瘤组织中没有发现。在埃利希瘤和吉田肉瘤的病例中，分别给予^<201 b> tl -氯化物，^<86> rb -氯化物和^<134> cs -氯化物，这些核素在活的肿瘤组织中积累了大量，在结缔组织中只积累了少量，无论给药后的时间如何，在坏死的肿瘤组织中未见。在注射了^<22>Na-chloride的肿瘤中，坏死肿瘤组织中^<22>Na的浓度高于存活肿瘤组织和结缔组织，与注射后的时间无关。根据我们的实验数据，我们得出^<67>Ga摄取到炎性病变的机制如下：炎性病变中_<67>Ga的积累主要是由于_<67>Ga通过通透性增加的毛细血管渗漏到中性粒细胞和巨噬细胞浸润的皮下组织。在炎性病变中，_<67>Ga优先与构成细胞间物质的酸性粘多糖结合。

项目成果

Atsushi Ando and Itsuko Ando: "Biodistributions of radioactive bipositive metal ions in tumor-bearing animals" BioMetals. (in press). (1994)

Atsushi Ando 和 Itsuko Ando：“放射性双阳性金属离子在荷瘤动物中的生物分布”BioMetals。

Atsushi Ando and Itsuko Ando: "Biodistributions of radioactive bipositive metal ions in tumor-bearing animals." BioMetals. 7(印刷中). (1994)

Atsushi Ando 和 Itsuko Ando：“携带肿瘤的动物中放射性双阳性金属离子的生物分布”（BioMetals）7（出版中）。

Atsushi Ando and Itsuko Ando: "Biodistributions of radioactive bipositive metal ions in tumor-bearing animals" BioMetals. 7(印刷中). (1994)

Atsushi Ando 和 Itsuko Ando：“携带肿瘤的动物中放射性双阳性金属离子的生物分布”BioMetals 7（出版中）。