Expression of althrnatively spliced src messenger RNAs related to neuronal differentiation in human neurblastoma

人神经母细胞瘤中与神经元分化相关的选择性剪接 src 信使 RNA 的表达

• 批准号: 04670724
• 负责人: OHNUMA Naomo
• 金额: $ 1.34万
• 依托单位: Chiba University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for General Scientific Research (C)
• 财政年份: 1992
• 资助国家: 日本
• 起止时间: 1992 至 1993
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-04670724/
• 关键词: c-src protooncogene; Neuroblastoma; Alternative splicing; neural differentiation; src遺伝子

Neuron specific stc mRNAs, expressed in human brain tissue by alternative splicing, are related to neural differentiation. The ability of neuroblastoma to mature colud be associated with neuronal c-srcN1 expression, and c-srcN2 mRNA is induced in chemically differentiated neuroblastoma cells in vitro. The prognosis of patient with neuroblastoma is strongly affected by the ability of the tumor to differentiate in vivo. Toward clarification of the relationship between neuronal src mRNAexpression and clinical feature of neutoglastoma, we analyzed 28 human neuroblastoma tissues for expression of src mRNAs by S1 nuclease protection assay, N-myc gene amplification was also examived by Southern blot hybridization and the clinical significance of neuronal src mRNA and its relevance to N-myc gene amplification were inbestigated. Expression of c-srcN2 mRNA at high ratio (more than 10 %)were at advanced stage and had aggressive phenotype in clinical course. Genomic amplification of N-myc gene and expression of c-srcN2 mRNA were correlated inversely. Expression of c-srcN2 mRNA could be a new biological marker to predict the prognosis of patients with neuroblastoma when combined with other prognostic marker such as N-myc gene amplification.

神经元特异性 stc mRNA 通过选择性剪接在人脑组织中表达，与神经分化相关。神经母细胞瘤成熟的能力可能与神经元c-srcN1表达有关，并且c-srcN2 mRNA在体外化学分化的神经母细胞瘤细胞中被诱导。神经母细胞瘤患者的预后很大程度上受肿瘤体内分化能力的影响。为了阐明神经元 src mRNA 表达与神经母细胞瘤临床特征之间的关系，我们通过 S1 核酸酶保护试验分析了 28 例人神经母细胞瘤组织中 src mRNA 的表达，并通过 Southern blot 杂交检测了 N-myc 基因扩增，并探讨了神经元 src mRNA 的临床意义及其与 N-myc 基因扩增的相关性。 怂恿的。 c-srcN2 mRNA高比例表达（10%以上）处于晚期，临床过程中具有侵袭性表型。 N-myc 基因的基因组扩增与 c-srcN2 mRNA 的表达呈负相关。当与其他预后标志物（如 N-myc 基因扩增）结合时，c-srcN2 mRNA 的表达可能成为预测神经母细胞瘤患者预后的新生物标志物。

项目成果

T.Matsunaga,N.Ohnuma et al: "Expression of alternatively spliced src messenger RNAs related to neuronal differentiation in human neuroblastoma" Cancer Research. 53. 3179-3185 (1993)

T.Matsunaga、N.Ohnuma 等人：“与人神经母细胞瘤中神经元分化相关的选择性剪接 src 信使 RNA 的表达”癌症研究。

松永 正訓,白澤 浩,田辺 政裕,大沼 直躬 他: "神経芽腫の分化とsrc mRNAのスプライシング" 第51回日本癌学会総会号. 143- (1992)

Masanori Matsunaga、Hiroshi Shirasawa、Masahiro Tanabe、Naomi Onuma 等：“神经母细胞瘤的分化和 src mRNA 的剪接”第 51 届日本癌症协会年会 143-（1992 年）。

Tadashi Matsunaga, Hideyo Takahashi, Naomi Ohnuma, Masahiro Tanabe, Jun Iwai, Hideo Yoshida, Hideki Enomoto, Hiroshi Horie, Hiroshi Sirasawa and Bunsiti Simizu: "Paratesticular meuroblastoma with N-myc activation" Journal of Pediatric Surgery. 28. 1612-16

Tadashi Matsunaga、Hideyo Takahashi、Naomi Ohnuma、Masahiro Tanabe、Jun Iwai、Hideo Yoshida、Hideki Enomoto、Hiroshi Horie、Hiroshi Sirasawa 和 Bunsiti Simizu：“伴有 N-myc 激活的甲状睾丸神经母细胞瘤”《小儿外科杂志》。

T Matsunaga,N Ohnuma et al: "Expression of alternatively spliced src messenger RNAs related to neuronal differentiation in human neuroblastoma" Cancer Research. 53. 3179-3185 (1993)

T Matsunaga、N Ohnuma 等人：“与人神经母细胞瘤中神经元分化相关的选择性剪接 src 信使 RNA 的表达”癌症研究。

松永正訓,大沼直躬他: "神経芽腫の分化能と神経特異的srcmRNAの発現" 小児外科. 25. 851-856 (1993)

Masanori Matsunaga、Naomi Onuma 等人：“神经母细胞瘤的分化能力和神经特异性 srcmRNA 的表达”《小儿外科》25. 851-856 (1993)。