In vivo observation of coronary microcirculation using a needle-probe video-microscope with a CCD camera during cardiac surgery

心脏手术期间使用带有 CCD 摄像头的针探针视频显微镜对冠状动脉微循环进行体内观察

基本信息

  • 批准号:
    04670844
  • 负责人:
  • 金额:
    $ 1.34万
  • 依托单位:
  • 依托单位国家:
    日本
  • 项目类别:
    Grant-in-Aid for General Scientific Research (C)
  • 财政年份:
    1992
  • 资助国家:
    日本
  • 起止时间:
    1992 至 1993
  • 项目状态:
    已结题

项目摘要

To evaluate the microcirculation of the human myocardium morphologically and functionally, we developed a portable needle-probe video-microscope with a charge coupled device (CCD) camera was applicable to the coronary microvessels during a cardiac surgery. First, using open-chest anesthetized pigs and dogs, we aimed to find technological hurdles for introducing the system during a surgery. Then, we developed several pieces of equipment to overcome the methodological problems. In brief, a sheathed needle-probe with a doughnut-shaped balloon was developed to avoid direct compression of the microvessels by the tip of the needle. To maintain a physiological condition of myocardial microvessels and the surrounding tissue during observation, a system consisting of a heat exchanger and a microtube was developed for flushing away the intervening blood between the tip of the needle and the myocardium. Using these devices, images of the myocardial microvessels of a beating heart could be obtained for a time which was long enough for imaging study. Furthermore, a holding device of the portable needle-probe video-microscope with a CCD camera was developed, which allowed an operator easy handling. This holding device contributed to shorten observation time. For the myocardial microvessels to be visualized without any special intravascular contrast medium, we employed a green (complimentary color of red) filter to accentuate the contrast between the images of the blood filled vessel and the surrounding tissue. Then, not only the coronary arterioles, but also coronary venules could be visualized. We confirmed that the portable needle-probe video-microscope with a CCD camera was able to visualize human coronary microvessels using all these supporting systems.We also evaluated the effects of nitroglycerin microvessels. The study showed that nitroglycerin dilates both subepicardial subendocardial arterioles. It was also found that nitroglycerin dilated coronary venules.
为了从形态学和功能学上评价人体心肌微循环,我们研制了一种便携式带电荷耦合器件(CCD)摄像头的针探头视频显微镜,适用于心脏手术中的冠状动脉微血管。首先,使用开胸麻醉的猪和狗,我们的目标是找到在手术过程中引入该系统的技术障碍。然后,我们开发了几种设备来克服方法上的问题。简而言之,开发了具有甜甜圈形球囊的护套针探针,以避免针尖直接压迫微血管。为了在观察过程中保持心肌微血管和周围组织的生理状态,开发了一种由热交换器和微管组成的系统,用于冲洗针尖和心肌之间的介入血液。使用这些设备,图像的心肌微血管的跳动的心脏,可以获得足够长的时间成像研究。此外,还研制了一种带CCD摄像头的便携式针探针视频显微镜夹持装置,使操作者易于操作。该夹持装置有助于缩短观察时间。为了在没有任何特殊的血管内造影剂的情况下可视化心肌微血管,我们采用了绿色(红色的互补色)滤波器来强调充满血液的血管和周围组织的图像之间的对比度。这样,不仅可以看到冠状小动脉,而且可以看到冠状小静脉。我们证实,便携式针探针视频显微镜与CCD摄像机能够显示人类冠状动脉微血管使用所有这些支持系统,我们也评价了硝酸甘油微血管的效果。研究表明,硝酸甘油扩张了心外膜下和心内膜下的小动脉。同时发现硝酸甘油扩张冠状小静脉。

项目成果

期刊论文数量(50)
专著数量(0)
科研奖励数量(0)
会议论文数量(0)
专利数量(0)
Toyotaka Yada,et al.: "In vivo observation of subendocardial microvessels of the beating porcine heart using a needle-probe videomicroscope with a CCD camera" Circulation Research. 72. 939-946 (1993)
Toyotaka Yada 等人:“使用带有 CCD 摄像头的针头视频显微镜对跳动的猪心脏的心内膜下微血管进行体内观察”循环研究。
  • DOI:
  • 发表时间:
  • 期刊:
  • 影响因子:
    0
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  • 通讯作者:
Masami Goto, et al.: "Effect of intracorocnary nitroglycerin administration on phasic pattern and transmural distribution of flow during corocnary artery stenosis" Circulation. 85. 2296-2304 (1992)
Masami Goto 等人:“冠状动脉狭窄期间冠状动脉内给予硝酸甘油对血流的相位模式和跨壁分布的影响”循环。
  • DOI:
  • 发表时间:
  • 期刊:
  • 影响因子:
    0
  • 作者:
  • 通讯作者:
Osamu Hiramatsu,et al.: "Effect of cardiac cortraction on the perforated intramural arteriole and venule in the dog" Circulation. 88. 1757 (1993)
Osamu Hiramatsu 等人:“心脏收缩对狗穿孔的壁内小动脉和小静脉的影响”循环。
  • DOI:
  • 发表时间:
  • 期刊:
  • 影响因子:
    0
  • 作者:
  • 通讯作者:
Toyotaka Yada,et al: "In vivo observation of subendocardial microressels of the beating porcine heart using a needle-probe video microscope with a CCD camera" Circulation Research. 72. 939-946 (1993)
Toyotaka Yada 等人:“使用带有 CCD 摄像头的针头视频显微镜对跳动的猪心脏的心内膜下微血管进行体内观察”循环研究。
  • DOI:
  • 发表时间:
  • 期刊:
  • 影响因子:
    0
  • 作者:
  • 通讯作者:
Fumihiko Kajiya, et al.: "Velocity profiles and phasic flow patterns in the non-stenotic human left anterior descending coroanry artery during cardiac surgery" Cardiovascular Research. 27. 845-850 (1993)
Fumihiko Kajiya 等人:“心脏手术期间非狭窄人左冠状动脉前降支的速度分布和相流模式”心血管研究。
  • DOI:
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    0
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FUJIWARA Takashi其他文献

FUJIWARA Takashi的其他文献

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{{ truncateString('FUJIWARA Takashi', 18)}}的其他基金

A study on the mechanism of induction of transdifferentiation of tissue-resident fibroblasts into endothelial cells, aiming at regenerative medicine
诱导组织驻留成纤维细胞转分化为内皮细胞的机制研究,瞄准再生医学
  • 批准号:
    18591416
  • 财政年份:
    2006
  • 资助金额:
    $ 1.34万
  • 项目类别:
    Grant-in-Aid for Scientific Research (C)
An analysis of the induction mechanism of transdifferentiation of connective tissue-resident fibroblasts into endothelial cells of blood vessels
结缔组织成纤维细胞转分化为血管内皮细胞的诱导机制分析
  • 批准号:
    16591260
  • 财政年份:
    2004
  • 资助金额:
    $ 1.34万
  • 项目类别:
    Grant-in-Aid for Scientific Research (C)
Real-time measurement of nitric oxide in coronary circulation during ischemia reperfusion and analysis of pathophysiological effects
缺血再灌注时冠脉循环一氧化氮实时测量及病理生理效应分析
  • 批准号:
    08671553
  • 财政年份:
    1996
  • 资助金额:
    $ 1.34万
  • 项目类别:
    Grant-in-Aid for Scientific Research (C)
Intraoperative evaluation of pathophysiological role of coronary slosh phenomenon
冠状动脉晃动现象病理生理作用的术中评估
  • 批准号:
    06671366
  • 财政年份:
    1994
  • 资助金额:
    $ 1.34万
  • 项目类别:
    Grant-in-Aid for General Scientific Research (C)
Blood Flow Dynamics of Left Coronary Artery in Hypertrophied Human Heart
肥大的人心脏左冠状动脉的血流动力学
  • 批准号:
    01570796
  • 财政年份:
    1989
  • 资助金额:
    $ 1.34万
  • 项目类别:
    Grant-in-Aid for General Scientific Research (C)

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Basic research for the collaterogenesis in coronary circulation
冠脉循环中胶原蛋白生成的基础研究
  • 批准号:
    25893187
  • 财政年份:
    2013
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THE EFFECTS OF PHYSIOLOGICAL STRESS ON CORONARY CIRCULATION
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    7951255
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    2009
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    $ 1.34万
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Coronary Circulation-Cardiac Mechanics Coupling
冠状动脉循环-心脏力学耦合
  • 批准号:
    20300160
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THE EFFECTS OF PHYSIOLOGICAL STRESS ON CORONARY CIRCULATION
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    7625788
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THE EFFECTS OF PHYSIOLOGICAL STRESS ON CORONARY CIRCULATION
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    7378517
  • 财政年份:
    2006
  • 资助金额:
    $ 1.34万
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Multiphasic Mixture Modeling and Simulation of Coronary Circulation
冠状动脉循环的多相混合建模和模拟
  • 批准号:
    18360054
  • 财政年份:
    2006
  • 资助金额:
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Molecular Biologic Approach of Coronary Circulation and Coronary Endothelial Function in Patients with Cyanotic Heart Disease
紫绀型心脏病患者冠状动脉循环和冠状动脉内皮功能的分子生物学方法
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冠状动脉循环的肌肉反射控制
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    8096741
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Muscle Reflex Control of the Coronary Circulation
冠状动脉循环的肌肉反射控制
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    7868065
  • 财政年份:
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