Chemosensitivity test for malignant bone and soft tissue tumor.

恶性骨和软组织肿瘤的化疗敏感性试验。

• 批准号: 04670887
• 负责人: OGIHARA Yoshio
• 金额: $ 0.77万
• 依托单位: MIE UNIVERSITY
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for General Scientific Research (C)
• 财政年份: 1992
• 资助国家: 日本
• 起止时间: 1992 至 1994
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-04670887/
• 关键词: Chemosensitiritytest; Malignant tumor; Soft tissue sarema; Bloneytometry; Anitimor; Poliamin; Oncogene; Chemosensitirity test; malignant fare tumor; soft tissue sarcoma; flowcytomotry; mtitnior drug; oncogene; colony assay; chemotherapy; an

Chemosensitivity test is very useful method to detect the efficacy of chemotherapy for the patient with malignant bone and soft tissue tumor. In addition to the basical study of harvesting of tumor cells, study about histological findings and ploidy pattern with flowcytometry was performed. We reported correlation between the results and prognosis at the meeting of the Japan Orthopaedic Association. We analyzed antitumor effects of methotrexate, adriamycin, ifosfamide and methylglyoxal bis (cyclopentylamidinohydrazone) with osteosarcoma cell lines. The results that there were additional effects of general antitumor drug and metgylhlyoxal bis (cyclopentylamidinohydrazone) with different pharmacokinetics were reported. Now we have the results about analysis of effect to cell cycle by the method of using 5-Bromodeoxyuridine (BrdU), fluorescein isothiocyanate labeled anti-BrdU monoclonal antibody and propidium iodide with flowcytometry. It will be reported qt the meeting of the Japan Orthopaedic Association.

药敏试验是评价恶性骨与软组织肿瘤化疗疗效的重要方法。除了对肿瘤细胞的收获进行基础研究外，还进行了组织学检查和流式细胞仪倍体模式的研究。我们在日本骨科协会会议上报告了结果与预后之间的相关性。我们分析了甲氨蝶呤，阿霉素，异环磷酰胺和甲基乙二醛双（环戊脒基腙）与骨肉瘤细胞系的抗肿瘤作用。本文报道了一般抗肿瘤药物与不同药代动力学的甲基乙二醛双（环戊脒基腙）在体内有附加作用的结果。用5-溴脱氧尿苷（BrdU）、异硫氰酸荧光素标记的抗BrdU单克隆抗体和碘化丙啶流式细胞术分析对细胞周期的影响。这将在日本矫形协会的会议上报告。

项目成果

園田潤: "骨肉腫細胞に対するポリアミン生合成配置阻害剤(MGBCP)の抗腫瘍効果について" 三重医学. (in press).

Jun Sonoda：“论多胺生物合成构型抑制剂（MGBCP）对骨肉瘤细胞的抗肿瘤作用”Mie Medical（出版中）。

園田潤: "骨肉腫細胞に対するポリアミン生合成要素阻害剤(MGBCP)の抗腫瘍効果について" 三重医学. 39. (1995)

Jun Sonoda：“论多胺生物合成成分抑制剂（MGBCP）对骨肉瘤细胞的抗肿瘤作用”Mie Medical 39。（1995）

関口章司: "脂肪肉腫の予後因子に関する基礎医学的検討,組織亜型核DNA量解析と化学療法に対する感受性について" 第8回日本整形外科基礎学術集会.

Shoji Sekiguchi：“脂肪肉瘤预后因素、组织学亚型核DNA含量分析和化疗敏感性的基础医学研究”第8届日本骨科骨科基础学术会议。

SEKIGUTI S: "A Study of Prognostic Value in Liposarcoma of the extremities" MIE MEDICAL JOUNAL. (in press).

SEKIGUTI S：“四肢脂肪肉瘤预后价值的研究”MIE MEDICAL JOUNAL。

関口章司: "四肢発生の脂肪肉腫の予後因子に関する基礎医学的検討 -組織学的所見、核DNA量解析、PCNA染色と化学療法について" 三重医学. 39. (1995)

Shoji Sekiguchi：“肢体发育脂肪肉瘤预后因素的基础医学研究 - 组织学结果、核 DNA 量分析、PCNA 染色和化疗”Mie Medical 39。（1995）