Study on the pathogenesis and biological vehavior of malignant giant cell tumor of bone

骨恶性巨细胞瘤发病机制及生物学行为研究

• 批准号: 60480338
• 负责人: OGIHARA Yoshio
• 金额: $ 2.24万
• 依托单位: Mie University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for General Scientific Research (B)
• 财政年份: 1985
• 资助国家: 日本
• 起止时间: 1985 至 1987
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-60480338/
• 关键词: Bistogenesis of Giant Cell Tumor of Bone; Cell Culture of Giant Cell Tumor of Bone; ヒト骨巨細胞腫の組織起源; 悪性線維性組織球腫の組織起源

In order to study on the pathogenesis of giant cell tumor of bone(GCT), cytomorphological and cytofunctional studies with paraffin embbeded, frozen and/or call cultured materials of GCT, malignant giant cell tumor of bone(M・GCT) and malignant fibrous histiocytoma(MFH.... the close relationship of this tumor to GCT in pathogenesis is often discussed.) were undertaken.Experimental study I (in 1985) We have successfully continued transplantation of tumor cells derived from human GCT in nude mice and established a transplantation line. With this material, cytomorphological (electron microscopic) and cytofunctional(phagocytotic, immunohistochemical.... <alpha>-1 antitrypsin,<alpha>-1 antichymotrypsin and so on) observations were made. The results of this study implied that a giant cell of this tumor was made by coalescence of stromal cells and the stromal cell took its rise in histiocytic line.Experimental study II (in 1986) In order to emphasize th existence of a histiocytic character in t … More he transplanted cells, collagenase activity was studied. Markedly high activity was shown in those cells compared to cultured call of other tumors. For newly obtained materials of GCT (16 cases) above mentioned cytomorphological and cytofunctional observation were carried out. Phagocytosis and positive immunohistochemical stain were often found in giant cells, and asteroid or cuboid stromal cells, which alluded that they belonged to histioctic cell goup in origun.Experimental study III (in 1987) MFH is strongly supposed to be histiocytic origin by some authors. Characters of this tumor cells(13 cases of MFH) and two kinds of cultured fibroblasts as a control(fibromatosis and normal human fibroblast) were studied by the same method done in GCT group. The results of this study showed strong resemblance to that with GCT or M・GCT. For these three years eloquent circumstantial evidences telling the histogenesis of GCT have been gathered. However none of them is direct proof and convincing enough. Continuous studies is needed before we make a conclusive remark that GCT originate from histiocytic cell group. Less

为探讨骨巨细胞瘤(GCT)的发病机制，对石蜡包埋、冷冻和(或)呼叫培养的GCT、恶性骨巨细胞瘤(M·GCT)和恶性纤维组织细胞瘤(MFH)进行了细胞形态和细胞功能研究。这种肿瘤与GCT在发病机制上的密切关系经常被讨论。)实验研究I(1985年)我们成功地将人GCT来源的肿瘤细胞移植到裸鼠体内，并建立了移植系。用这种材料，细胞形态(电子显微镜)和细胞功能(吞噬，免疫组织化学...&lt；α&gt；-1抗胰蛋白酶、&lt；α&gt；-1抗凝乳糜酶等)。实验研究二(1986年)为了强调t-…中组织细胞的存在，实验研究二更多的移植细胞，胶原酶活性进行了研究。与培养的其他肿瘤细胞相比，这些细胞的活性明显增强。对新获得的GCT材料(16例)进行细胞形态和细胞功能观察。巨细胞、小行星或立方体基质细胞常见吞噬反应和免疫组织化学染色阳性，提示其起源属于组织细胞群。实验研究III(1987)部分作者认为MFH为组织细胞起源。用与GCT组相同的方法研究该肿瘤细胞(13例MFH)和两种培养的成纤维细胞作为对照(纤维瘤病和正常人成纤维细胞)的特性。本研究的结果与GCT或M·GCT的结果有很强的相似性。在这三年里，已经收集了雄辩的间接证据来说明GCT的组织起源。然而，它们都不是直接的证据和足够的说服力。在我们得出GCT起源于组织细胞群的结论之前，还需要进行持续的研究。较少

项目成果

加藤公: 三重医学. 29. 137-145 (1985)

加藤：三重医疗。29. 137-145 (1985)

Takeshi,SUMIMOTO: "Collagenase Activity of Human Malignant Giant Cell Tumor Serially Transplanted in Mude Mice" MIE-Igaku. 30. 147-152 (1986)

Takeshi，SUMIMOTO：“连续移植到小鼠体内的人类恶性巨细胞肿瘤的胶原酶活性”MIE-Igaku。

隅本毅: 三重医学. 30. 147-152 (1986)

住本武：三重医学科学。 30. 147-152 (1986)

Shyuichi,FUJINAMI: "A Study on the Origin of Malignant Fibrous Histiocytoma and Giant Cell Tumor of Bone"

藤波秀一：“恶性纤维组织细胞瘤和骨巨细胞瘤的起源研究”

舘靖彦: 三重医学.

泰特康彦：三重医疗。