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Histogenesis of Malignant Giant Cell Tumor and Malignant Fibrous Histiocytoma

恶性巨细胞瘤和恶性纤维组织细胞瘤的组织发生

基本信息

批准号：
01480367
负责人：
OGIHARA Yoshio
金额：
$ 1.86万
依托单位：
Mie University
依托单位国家：
日本
项目类别：
Grant-in-Aid for General Scientific Research (B)
财政年份：
1989
资助国家：
日本
起止时间：
1989 至 1991
项目状态：
已结题

项目摘要

The histogenesis of malignant fibrous histiocytoma (MFH) and giant cell tumor is controversial. To elucidate the cellular origin and characteristics of these neoplasms, we have studied many investigations. The findings of electron-microscopic observation, immunohistochemical study with polyclonal antibody and examination of phagocytosis suggested that the origin of MFH was histiocyte. But they don't have enough specificity to conclusion. In this time, we studied the production of collagen in cell cultures derived from these tumors. As the results, we proved the fact that tumor cells as well as histiocytes produced type III collagen. But as a result of immunohistochemical study of monoclonal antibody, negative reactions for anti-human macrophage, T200, anti-leu-M5, anti-leu-M3 and anti-leu-6 were demonstrated in tumor cells. Furthermore inducers of differentiation was added to the culture, but the results were same. In this method, necessity of a large number of tumor cells became a technical problem. In the study with flowcytometry, all cells which were contained in tumor tissue were divided in two groups according to cell size. The group of bigger cells developed a tendency to contain aneuploid cell and histiocytic cell. Cells with ability of mitosis and histiocytic cells tended to be aneuploid. In the in vivo study, thymus tissue was transplanted into nude mice with human malignant fibrous histiocytoma, Then monotonous histological findings of tumor tissue changed into pleomorphic one composed of inflammatory cells, fibroblastic cells and collagen substance. These findings support the hypothesis that origin of malignant fibrous histiocytoma is histiocyte and a part of histiocytic cells become facultative fibroblasts.

恶性纤维组织细胞瘤（MFH）和巨细胞瘤的组织发生存在争议。为了阐明这些肿瘤的细胞起源和特征，我们进行了许多研究。电镜观察、免疫组化多克隆抗体及吞噬检查提示MFH的来源为组织细胞。但它们没有足够的特异性来得出结论。在这段时间里，我们研究了从这些肿瘤中提取的细胞培养中胶原蛋白的产生。作为结果，我们证明了肿瘤细胞以及组织细胞产生III型胶原蛋白的事实。但单克隆抗体免疫组化研究结果显示，肿瘤细胞中抗人巨噬细胞、T200、抗leu- m5、抗leu- m3、抗leu-6均呈阴性反应。在培养基中加入分化诱导剂，但结果相同。在这种方法中，是否需要大量的肿瘤细胞成为一个技术问题。在流式细胞术的研究中，肿瘤组织中含有的所有细胞根据细胞大小分为两组。较大的细胞群倾向于含有非整倍体细胞和组织细胞。具有有丝分裂能力的细胞和组织细胞倾向于非整倍体。在体内研究中，将胸腺组织移植到患有人恶性纤维组织细胞瘤的裸鼠体内，肿瘤组织的单一组织学表现转变为由炎症细胞、成纤维细胞和胶原物质组成的多形性组织。这些发现支持了恶性纤维组织细胞瘤起源于组织细胞，部分组织细胞成为兼性成纤维细胞的假设。