EXPERIMENTAL STUDY OF PERIPHERAL NERVE ALLOGRAFT WITH ETHANOL PRETREATMENT

乙醇预处理同种异体周围神经的实验研究

• 批准号: 04670901
• 负责人: TAMAI Kazuo
• 金额: $ 1.34万
• 依托单位: KYOTO PREFECTURALUNIVERSITY OF MEDICINE
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for General Scientific Research (C)
• 财政年份: 1992
• 资助国家: 日本
• 起止时间: 1992 至 1994
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-04670901/
• 关键词: PERIPHERAL NERVE; ALLOGRAFT; ETHANOL; BASEMENTMEMBRANE; LAMININ

TO REDUCE THE IMMUNOLOGICAL REACTIONS AGAINST THE ALLOGRAFTED NERVE IS THE MAJOR PROBLEM ABOUT PERIPHERAL NERVE ALLOGRAFT.TWO METHODS ARE SUJESTED TO REDUCE IMMNOREACTIONS.ONE IS TO USE IMMUNOSUPPRESSIVEAGENT AND THE OTHER IS TO REDUCE THE ANTIGENISITY OF THE GRAFTED NERVE BY PRETREATMENT.OUR COLLEAGUE HIRASAWA PLANED TO REDUCE THE ANTIGENISITY BY ETHANOL PRETREATMENT AND REPORTED GOOD RENERVATION.BUT THE RESULT WAS INFERIOR TO THAT OF THE AUTOGRAFT.IN THIS STUDY,WE IMPROVED THE WAY OF THE PRETREATMENT.FIRST,GRAFTED NERVE WAS PRETREATED BY 50,70,90 100% ETHANOL GRADUALLY EVERY FOUR HOURS.STIFFNESS OF THE BASEMENTMEMBRANE WAS OBSERVED AND THE LOW IMMUNOREACTION OF THE ANTI-LAMININE ANTIGEN WAS DETECTED IN THE IMMUNOCYTOCHEMISTRY.AT THREE MONTHS AFTER GRAFTING,THE RENERVATION THROUGH THE GRAFTED NERVE WAS NOT SO GOOD.THEN THE GRAFTED NERVE WAS PRETREATED BY 50,70,90% ETHANOL EVERY FOUR HOURS TWO TIMES.STIFFNESS OF THE BASEMENTMEMBRANE WAS IMPROVED AND THE IMMUNOREACTION OF THE LAMININ WAS AS SAME AS THAT OF THE NORMAL NERVE.GOOD RENERVATION WAS OBSERVED HITOLOGICALLY AND ELECTROPHYSIOLOGICALLY.BUT THE MATURATION OF THE REGENERATED AXON AND THE AMPLITUDE OF THE CMAP WAS INFERIOR TO THE RESULTS OF THE AUTOGRAFTED NERVE,AND FURTHER EXAMINATION WILL BE NEEDED FROM NOW ON.

减少同种异体神经移植后的免疫反应是同种异体周围神经移植的主要问题。减少免疫反应的方法有两种：一种是使用免疫抑制剂，另一种是通过预处理来减少移植神经的抗原性。我们的同事Hirasawa计划用乙醇预处理来减少移植神经的抗原性，并报告了良好的神经再生。但结果不如自体移植。在本研究中，我们改进了预处理的方法，先用50、70、90、100%乙醇每4小时梯度预处理移植神经，观察移植神经基底膜的硬度和免疫细胞化学中抗层粘连蛋白抗原的低免疫反应，移植后3个月，移植神经的再神经化不佳，然后用50、70、90、100%乙醇预处理移植神经。实验结果表明，用90%乙醇浸泡自体神经，每4小时2次，基底膜硬度提高，层粘连蛋白的免疫反应与正常神经相似，神经再生良好，但再生轴突的成熟度和CMAP的幅度不及自体神经，有待进一步研究。

项目成果

平澤泰介: "整形外科基本手術手枝の進歩 神経縫合法の実際" OS NOW. 6. 110-121 (1992)

Taisuke Hirasawa：“基础骨科手术的进展：神经缝合的实践”OS NOW 6. 110-121 (1992)。

北條達也: "エタノール処理末梢神経同種移植に関する実験的研究" 中部整形災害外科学会雑誌. 37. 1223-1224 (1994)

Tatsuya Hojo：“乙醇处理的周围神经同种异体移植物的实验研究”中部整形外科学会杂志 37. 1223-1224 (1994)。

北條達也: "エタノール処理末梢神経同種移植に関する実験的研究" 中部日本整形災害外科学会誌. 37. 1223-1224 (1994)

Tatsuya Hojo：“乙醇处理的周围神经同种异体移植物的实验研究”日本中部整形外科学会杂志 37. 1223-1224 (1994)。

TATSUYA HOJO: "HISTOLOGICAL AND ELECTROPHYSIOLOGICAL STUDIES OF PERIPHERAL NERVE ALLOGRAFTS USING IMMUNOSUPPRESSIVE AGENT" J.KYOTO PREF.UNIV.MED.103 (11). 1259-1271 (1994)

TATSUYA HOJO：“使用免疫抑制剂对周围神经同种异体移植物进行组织学和电生理学研究”J.KYOTO PREF.UNIV.MED.103 (11)。

SEIICHIRO OKAJIMA: "SYNAPTOPHYSIN IMMUNOCYTOCHEMISTRY IN THE REGENERATING SPROUTS FROM THE NODES OF RANVIER IN THE INJURED RAT SCIATIC NERVE" BRAIN RESEARCH. 631. 133-136 (1994)

Seiichiro OKajima：“受损大鼠坐骨神经朗维尔结再生芽中的突触素免疫细胞化学”脑研究。