An expetinental study onthe cause of the compression myelopathy and radiculopathy

压迫性脊髓病和神经根病病因的实验研究

基本信息

批准号：
04670907
负责人：
FUJIMURA Yoshikazu
金额：
$ 1.28万
依托单位：
Keio University
依托单位国家：
日本
项目类别：
Grant-in-Aid for General Scientific Research (C)
财政年份：
1992
资助国家：
日本
起止时间：
1992 至 1993
项目状态：
已结题

项目摘要

The fumctionsl changes of the motor neurons is one of the reasons that compression muslopathy and radiculopathy. Choline acetyltransferase(CAT), the final enzyme in acetylcholine synthesis, is known as the most specific marker for cholinergic meurons and it's expression sensitively reflects motor neuron function. In our study, changes in CAT distribution in the rat spinal cord caused by the chronic or acute compression were investigated by quantitating indirect CAT immunofluorescence of the spinal cord sections using a microphotometru system.Twy mice were used as a chronic compression model. There was th chronic compression at the upper cervical spinal cord, the grage of which increaswd gradually according to their week-age. Twy mice were classified to the three groups as follows by the compression : Group1, mild compression ; Group2, moderate compression ; group3 sever compression. Ther were no differences of the averaged fluorescence intensities of the antero-medial nucleus of the anterior horn. which reflected the concentration of CAT, bbetween creased to 76% of those of Group 1. Acute spinal cord injury at C6 level of rats, produced by the weight-placed method, resulted in a severe motor functional deficit initially, followed by a gradual recovery. At C6 level, the fluorescence intensity of the ventrolateral anterior horn(VLAH), decreased to 50% of that of sham-operated group at 2 days, It then recoverd to 60% at 4 days after injury, and remained umchanged thereafter. Fluorescence intensisies in VLAH at C4-5 and C7-8 levels decreased to 60-70% at 2 days after injury, but it recoverd and increased to 105-120% thereafter. A strong correlation was found betweenthe late excessive recvery of the fluorescence intensity in VLAH both rostral and caudal to the injury and the jotor functional recvery.

运动神经元的FumCtionsL变化是压缩肌瘤和根本性病变的原因之一。胆碱乙酰基转移酶（CAT）是乙酰胆碱合成中的最终酶，被称为胆碱能神经元的最特异性标记物，其表达敏感地反映了运动神经元功能。在我们的研究中，通过使用微电位术系统对间接CAT免疫荧光进行定量，研究了由慢性或急性压缩引起的大鼠脊髓中的CAT分布的变化。使用Microphotometru System.twy小鼠用作慢性压缩模型。上颈脊髓上有慢性压缩，其颗粒根据其周的年龄逐渐增加。 TWY小鼠被分类为三组，如下所示：组1，轻度压缩；组2，中等压缩； Group3严重压缩。这没有前角前核核的平均荧光强度的差异。这反映了CAT的浓度，cAT的浓度是在第1组中的76％之间。急性脊髓损伤在C6的大鼠水平上，由重量放置的方法产生，最初导致严重的运动功能不足，然后逐渐恢复。在C6水平上，腹侧前角（VLAH）的荧光强度降低至假手术组的50％，然后在受伤后4天后恢复至60％，然后在此后保持不变。受伤后2天，在C4-5和C7-8时，VLAH的荧光强度下降到60-70％，但恢复并增加到105-120％。发现vlah荧光强度的过度恢复与损伤和Jotor功能恢复相关。