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An expetinental study onthe cause of the compression myelopathy and radiculopathy

压迫性脊髓病和神经根病病因的实验研究

基本信息

批准号：
04670907
负责人：
FUJIMURA Yoshikazu
金额：
$ 1.28万
依托单位：
Keio University
依托单位国家：
日本
项目类别：
Grant-in-Aid for General Scientific Research (C)
财政年份：
1992
资助国家：
日本
起止时间：
1992 至 1993
项目状态：
已结题

项目摘要

The fumctionsl changes of the motor neurons is one of the reasons that compression muslopathy and radiculopathy. Choline acetyltransferase(CAT), the final enzyme in acetylcholine synthesis, is known as the most specific marker for cholinergic meurons and it's expression sensitively reflects motor neuron function. In our study, changes in CAT distribution in the rat spinal cord caused by the chronic or acute compression were investigated by quantitating indirect CAT immunofluorescence of the spinal cord sections using a microphotometru system.Twy mice were used as a chronic compression model. There was th chronic compression at the upper cervical spinal cord, the grage of which increaswd gradually according to their week-age. Twy mice were classified to the three groups as follows by the compression : Group1, mild compression ; Group2, moderate compression ; group3 sever compression. Ther were no differences of the averaged fluorescence intensities of the antero-medial nucleus of the anterior horn. which reflected the concentration of CAT, bbetween creased to 76% of those of Group 1. Acute spinal cord injury at C6 level of rats, produced by the weight-placed method, resulted in a severe motor functional deficit initially, followed by a gradual recovery. At C6 level, the fluorescence intensity of the ventrolateral anterior horn(VLAH), decreased to 50% of that of sham-operated group at 2 days, It then recoverd to 60% at 4 days after injury, and remained umchanged thereafter. Fluorescence intensisies in VLAH at C4-5 and C7-8 levels decreased to 60-70% at 2 days after injury, but it recoverd and increased to 105-120% thereafter. A strong correlation was found betweenthe late excessive recvery of the fluorescence intensity in VLAH both rostral and caudal to the injury and the jotor functional recvery.

运动神经元功能的改变是压迫性肌病和神经根病的原因之一。胆碱乙酰转移酶(CAT)是乙酰胆碱合成的终末酶，被认为是胆碱能神经元最特异的标志物，它的表达能灵敏地反映运动神经元的功能。本研究以2只小鼠为慢性压迫模型，通过显微图像分析系统对脊髓切片中CAT的间接免疫荧光进行定量，研究慢性压迫和急性压迫对大鼠脊髓内CAT分布的影响。在上颈髓有慢性压迫，其程度随周龄增加而逐渐增大。将Twy小鼠按受压情况分为三组：轻度受压组1、中度受压组、重度受压组。前角前内侧核的平均荧光强度无差异。反映了CAT的浓度，两者之间增加到组1的76%。负重法造成大鼠C6水平的急性脊髓损伤，最初导致严重的运动功能障碍，然后逐渐恢复。在C6水平，脊髓腹外侧前角(VLAH)的荧光强度在伤后2天降至假手术组的50%，伤后4天恢复至假手术组的60%，此后无明显变化。VLAH中C4-5和C7-8水平的荧光强度在伤后2天下降到60-70%，但随后恢复，此后增加到105-120%。VLAH吻侧和尾侧VLAH的荧光强度在损伤后后期过度恢复与伸肌功能恢复有很强的相关性。