An experimental study for the functional recovery of injured spinal motoneurons

我们研究了神经退行性因素对急性脊柱损伤模型中损伤性马达的影响。我们对以下两个实验的研究估计1.25小白鼠在这个实验中使用过。一个不完整的脊柱损伤模型是由在C6级5分钟内放置20克重量的Dura mater（n=20），在受伤后的第3、7、14、28天去除的，冻结部分被保留在p-75中。p-75的正摩托车计数。（结果）Sham组中没有P-75阳性摩托车（n=5）。在伤害组中，p-75积极的摩托车在伤害后第3天被识别，在第7天被高度识别，在第14天和第28天被高度认可。20个不完整的脊柱损伤模型是在同一个咒语中创造的。在10个细胞中，神经元衍生因子（GDNF）在伤害后直接进入了亚基底空间，而磷酸盐缓冲液（PBS）在其他10个动物体内。汽车功能在受伤后第2天和第7天被评估。脊柱的和弦在7天后从两个团体中消失了。The sections were immunofluorescence stained for choline acetyltransferase （ChAT）。CHAT荧光是用一个显微光度学系统来衡量的。（结果）发动机功能评估：GDNF组和PBS组都可以在受伤前保持他们的主干线，达到82岁的平均角度。关于受伤后的第二天，他们在哪里的角度，很有可能维护他们的行李箱到55岁和52岁，尊重地，到第7天，到68岁和63岁，尊重地。第二天对后受伤的影响并不明显，但第七天有明显的影响。免疫力化学发现：两个受伤害群体中的荧光价值低于不受伤害群体中的荧光价值，但GDNF群体中发生的荧光价值的减少是一种明显的预防。

We investigated the influences of neurotrophic factor on injured motoneurons in acute spinal cord injury models. Our study consists of the following two experimentsExperiment 1.25 Wister rats were used in this experiment. An incomplete spinal cord injury model was created by placing a 20-g weight on the dura mater at the C6 level for 5 min (n=20) .The cervical spinal cord was remove at post-injury day 3,7,14,28, and frozen sections were stained for p-75 .The number of p-75 positive motoneurons were counted. (Results) There were no p-75 positive motoneurons in the sham group(n=5). In the injury group, p-75 positive motoneurons were recognized at post-injury day 3, highly increased at day 7, and hardly observed by at day 14, 28.Experiment. 2; 20 incomplete spinal cord injury models were created in the same manner. In 10 glial cell line-derived neurotrophic factor(GDNF) was injected into the subarachnoid space immediately after the injury, ,and phosphate buffer saline (PBS) was injected n the other 10 animals. Motor function was evaluated at post-injury day 2 and 7. The spinal cord was removed from both groups 7 days after the injury. The sections were immunofluorescence stained for choline acetyltransferase (ChAT). ChAT fluorescence was measured with a microphotometry system. (Results) Motor function evaluation: Both the GDNF group and the PBS group were able to maintain their trunk on the inclined plane up to a mean angle of 82゜before the injury. On post-injury day 2 the angle at which they, were able to maintain their trunk had decreased to 55゜and 52゜, respectively, and on day 7, to 68゜and 63゜ respectively. The deference on post-injury day 2 was not significant, but a significant deference was observed by day 7. Immunohistochemical findings: The fluorescence values in both injured groups were lower than in the uninjured group, but the reduction in fluorescence that occurred in the GDNF group was a significantly prevented.