Hoemeostais of inne ear on the point of non-specic defense

本实验通过对内耳病理未知疾病的研究，阐明了内耳对病原菌的非特异性防御机制（第一防御机制）。将K-562骨髓性白血病细胞经圆窗膜注入豚鼠淋巴周围间隙，观察第1 ~ 9天内耳反应。第7天，在大部分K-562细胞完好无损的情况下，淋巴周围出现了抗亚洲草GM1阳性的NK细胞。第9天淋巴周围出现大量的K-562细胞，并伴有贴壁的炎症细胞，且大部分发生变性。NK细胞在交感周围空间的出现要比在皮肤中晚得多，皮肤也注射了K-562细胞。这种延迟有可能在NK细胞动员之前导致耳蜗因病原体而发生不可逆转的变化。此外，内耳液中的体液因子对病毒感染的抑制作用不强，内淋巴囊在防御这种病原体以挽救耳蜗方面似乎没有发挥重要作用。因此，内耳似乎是一个相对难以让免疫活性细胞发挥功能的环境。用免疫组织学方法研究了听力学损伤对豚鼠耳蜗循环的影响。卡那霉素被用作血流示踪剂。此外，通过组织化学检查确定听损伤后耳蜗内自由基的出现情况。血管纹的血流量大大减少。声损伤的原因尚不清楚。但超氧阴离子自由基（O_2）可能与试验血液循环有关，影响试验功能。

In this experiment ethiologically unnown iner ear disease has been studied to clarify the non-specific defense mechanism (first defense mechanism) of the inner ear against pathogen. K-562 myelogenous leukemia cells were injected into the guinea pig perilymphatic space through the round window membrane and the raction of the inner ear was observed on day 1 to day 9. NK cells which were positive for anti-asialo GM1 appared in the perilymphatic space on day 7 when most of K-562 cells were still intact. On day 9 there were many K-562 cells with adherent phlogocytes in the perilymph and were mostly degenerated. The emergence of NK cells was much later in the perilympahtic space than in the skin which was also injected with K-562 cells. And there is a possibility that this delay can lead to the irreversiable change of cochlea due to pathogens before the mobilization of NK cells. In addition, the humoral factors in the inner ear fluid was not so powerful in suppresing viral infection and the endolympohatic sac didnot seem to play an important role in the defense against this pathiogen to rescue the cochlea. In consequence, the inner ear seems to be an environment which is relatively difficult for immunocompetent cells to perform their functions.The effect of acoustic trauma on cochlear strial circulation was investigated imuunohistologically in the guinea pig. Kanamycin was used as a tracer of blood flow. Besides, histochemical examination were made to ascertain the emergence of free radicals in the cochlea after acoustic trauma. The blood flow in the stria vascularis was greatly diminished. The cause of acoustic trauma is obscure. But superoxide anion radicals (O_2) may be related to strial blood cieculation and affect strial function.