Hoemeostais of inne ear on the point of non-specic defense

内耳非特异性防御​​点的 Hoemeostais

• 批准号: 04671047
• 负责人: YAMANE Hideo
• 金额: $ 1.34万
• 依托单位: OSAKA CITY UNIVERSITY
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for General Scientific Research (C)
• 财政年份: 1992
• 资助国家: 日本
• 起止时间: 1992 至 1994
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-04671047/
• 关键词: Inner ear; Cochlea; Stria vascularis; Non-specific defense; Free radical; NK cell; Acoustic trauma; K-562; Inner ear; tirst detense; tree radical; NK cell; strial blood flow; 非特異的防御機構; ナチュラルキラー細胞; 音響外傷

In this experiment ethiologically unnown iner ear disease has been studied to clarify the non-specific defense mechanism (first defense mechanism) of the inner ear against pathogen. K-562 myelogenous leukemia cells were injected into the guinea pig perilymphatic space through the round window membrane and the raction of the inner ear was observed on day 1 to day 9. NK cells which were positive for anti-asialo GM1 appared in the perilymphatic space on day 7 when most of K-562 cells were still intact. On day 9 there were many K-562 cells with adherent phlogocytes in the perilymph and were mostly degenerated. The emergence of NK cells was much later in the perilympahtic space than in the skin which was also injected with K-562 cells. And there is a possibility that this delay can lead to the irreversiable change of cochlea due to pathogens before the mobilization of NK cells. In addition, the humoral factors in the inner ear fluid was not so powerful in suppresing viral infection and the endolympohatic sac didnot seem to play an important role in the defense against this pathiogen to rescue the cochlea. In consequence, the inner ear seems to be an environment which is relatively difficult for immunocompetent cells to perform their functions.The effect of acoustic trauma on cochlear strial circulation was investigated imuunohistologically in the guinea pig. Kanamycin was used as a tracer of blood flow. Besides, histochemical examination were made to ascertain the emergence of free radicals in the cochlea after acoustic trauma. The blood flow in the stria vascularis was greatly diminished. The cause of acoustic trauma is obscure. But superoxide anion radicals (O_2) may be related to strial blood cieculation and affect strial function.

本实验以一种在人类学上尚未发现的内耳疾病为研究对象，旨在阐明内耳对病原体的非特异性防御机制（第一防御机制）。将K-562髓性白血病细胞经圆窗膜注入豚鼠外淋巴间隙，观察第1 ~ 9天内耳反应。在第7天，当大多数K-562细胞仍然完整时，外淋巴间隙中的抗脱唾液酸GM 1抗体阳性的NK细胞。第9天外淋巴液中有大量K-562细胞与炎细胞粘附，且大多数细胞变性。NK细胞在淋巴管外隙中的出现比在皮肤中晚得多，皮肤也注射了K-562细胞。这种延迟可能导致在NK细胞动员之前由于病原体引起的耳蜗的不可逆变化。此外，内耳液中的体液因子对病毒感染的抑制作用不强，内淋巴囊在抵抗病毒感染以挽救耳蜗中似乎不起重要作用。因此，内耳似乎是一个环境，这是一个相对困难的免疫活性细胞履行其职能。卡那霉素被用作血流的示踪剂。此外，还进行了组织化学检查，以确定自由基在听损伤后耳蜗中的出现。血管纹内血流明显减少。声损伤的原因尚不清楚。但超氧阴离子自由基（O_2）可能与血液循环有关，影响机体功能。

项目成果

井口広義、山根英雄、小西一夫、坂本平守、中川隆之、柴田太郎、高山雅裕、西村啓三、川勝さわ子、角田貴司子、中井義明、高橋研一: "内耳の非特異的防御機構-第3報-" Otal Jpn. 3. 615 (1993)

Hiroyoshi Iguchi、Hideo Yamane、Kazuo Konishi、Tairamori Sakamoto、Takayuki Nakakawa、Taro Shibata、Masahiro Takayama、Keizo Nishimura、Sawako Kawakatsu、Takashiko Tsunoda、Yoshiaki Nakai、Kenichi Takahashi：“内耳的非特异性防御​​机制 - 第三次报告 - “Otal Jpn. 3. 615 (1993)

井口広義、中井義明、山根英雄、: "内耳の局部防御機構に関する基礎的研究-特に非特異的防御機構について-" Eqailibriun Res Suppl. 10. 37-53 (1994)

Hiroyoshi Iguchi、Yoshiaki Nakai、Hideo Yamane：“内耳局部防御机制的基础研究 - 特别是关于非特异性防御​​机制”Eqailibriun Res Suppl. 10. 37-53 (1994)

井口広義、山根英雄、坂本平守、小西一夫、中川隆之、森中耕介、高山雅裕、中井義明: "内耳膜迷路の分裂と分化(第3報)" Otol Jpm. 2. 139 (1992)

Hiroyoshi Iguchi、Hideo Yamane、Hiramori Sakamoto、Kazuo Konishi、Takayuki Morinaka、Masahiro Takayama、Yoshiaki Nakai：“内耳迷路的划分和分化（第 3 次报告）” Otol Jpm。

H.Yamane, Y.Nakai, M.Takayama, K.Konishi, H.Iguchi, T.Nakagawa: "Strial blood circulation and acoustic trauma" Otol Jpn. 3 (3). 288-294 (1993)

H.Yamane、Y.Nakai、M.Takayama、K.Konishi、H.Iguchi、T.Nakakawa：“心房血液循环和声损伤” Otol Jpn。

H.Yamane, H.Iguchi, Y.Nakai, T.Nakagawa, K.Konioshi, K.Nakagawa, M.Takayama, K.Takahashi: "The first defense of the inner ear." Immunobiology in otorhinolaryngology-Progress of Decade.PP165-174 Karger. (1994)

H.Yamane、H.Iguchi、Y.Nakai、T.Nakakawa、K.Konioshi、K.Nakakawa、M.Takayama、K.Takahashi：“内耳的第一道防御。”