Research for optic nerve damage in glaucoma

青光眼视神经损伤的研究

• 批准号: 04671067
• 负责人: SAWAGUCHI Shoichi
• 金额: $ 0.51万
• 依托单位: Niigata University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for General Scientific Research (C)
• 财政年份: 1992
• 资助国家: 日本
• 起止时间: 1992 至 1993
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-04671067/
• 关键词: Fast axonal transport; Slow axonal transport; Experimental glaucoma; 軸索輸送障害

We studied the axonal transport damage in experimentally induced primate glaucoma and its relation to glaucomatous optic nerve injury.Fast axonal transport was studied in glaucomatous eyes using the canulation method to the anterior chamber. The amount of axonal transport was reduced dramatically in experimental eyes due to the loss of retinal ganglion cells. Also showed the minimum axonal transport blockage at the level of lamina cribrosa. The results demonstrated unfortunately too miumum to show up under routine light microscope.Slow axonal transport, however, demonstrated clear blockage at the lamina cribrosa. Furthrmore, such axonal transport blockage was seen even retro-laminar portion of optic nerve. The result add new information about axonal transport damage, since so far axonal transport blockage was reported to be seen at the level of lamina cribrosa in acute IOP elevation.We reported the data to the Japanese Glaucoma journal 1994.We are still examining the fast axonal transport abnormality in experimental glaucoma using the dark field microscopy to show the clear difference between normal and glaucomatous eyes.Slow axonal transport data was not impressive enough since the color of silver grains are not totally black and tissue autoradiography should be repeated.We agreed that comment and emulsion coated tissue are now in the dark and cold box for development. The time to be developed will be between May and June. The all data will be completed around August 95' for final revision.

本研究采用前房插管法观察了青光眼视神经轴突的快速运输，并探讨了青光眼视神经轴突运输损伤与青光眼视神经损伤的关系。由于视网膜神经节细胞的丢失，实验眼的轴突运输量显著减少。在筛板水平也显示了最小的轴突运输阻塞。结果显示，在常规光学显微镜下，轴突运输速度慢，但在筛板处有明显的阻塞。此外，这种轴突运输障碍甚至可见于视神经板后部分。这一结果增加了关于轴突运输损伤新信息，本文报道了急性高眼压时筛板水平的轴索运输障碍，并将其报道于日本青光眼杂志1994年。我们仍在用暗视野显微镜检查实验性青光眼的快轴索运输异常，以显示正常眼和青光眼眼之间的明显差异。慢轴索运输数据显示，青光眼眼的快轴索运输异常与青光眼眼的快轴索运输异常之间存在明显差异，而青光眼眼的慢轴索运输异常与青光眼眼的快轴索运输异常之间存在明显差异。由于银颗粒的颜色不完全是黑色的，组织放射自显影应重复进行，因此给人的印象不够深刻。我们同意，注释和乳剂涂层组织现在处于黑暗和冷箱中进行显影。开发时间将在5月至6月之间。所有数据将于1995年8月左右完成，以便最终修订。

项目成果

沢口昭一: "猿実験緑内障におけるSlow Axonal Transportの障害" 日本緑内障学会雑誌. 第4巻. 167- (1994)

Shoichi Sawaguchi：“猴子实验性青光眼的慢速轴突运输受损”，日本青光眼协会杂志，第 4 卷，167-（1994 年）。

SHOICHI SAWAGUCHI: "Slow Axonal Transport blockage in Experimental primate Glaucoma" Journal of Japan Glaucoma Society. Vol.4. 167 (1994)

SHOICHI SAWAGUCHI：“实验灵长类青光眼中的缓慢轴突运输阻塞”日本青光眼协会杂志。