Expression of heat shock proteins in the monkey eyes

热休克蛋白在猴眼中的表达

• 批准号: 13671845
• 负责人: SAWAGUCHI Shoichi
• 金额: $ 1.22万
• 依托单位: University of the Ryukyu
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2001
• 资助国家: 日本
• 起止时间: 2001 至 2002
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-13671845/
• 关键词: Experimental primate glaucoma; Heat shock protein; Retina; Optic nerve head; Histochemistry; 実験緑内障; ヒートショック蛋白; ISH; 細胞内マトリックス

1. Expression of Heat Shock Protein in the primate glaucomatous retinaExperimental glaucoma model was made by repeated exposure of argon laser to the chamber angle. Intraocular pressure and optic nerve head was then monitored.Immunohistochemistry was performed for panels of antibodies against HSPs.Neuroprotective HSP (90,60,27) were upregulated in the glaucomatous retina compared with that of control normal retina. HSP 70 was also moderately elevated in the glaucomatous retina. Expression of HSP 47, act to extracellular material remodeling was not altered in glaucomatous retina. Such immunoreactivity was quantificated by Zeiss DAB analysis system and significantly change was obtained for all HSP molecules except that of HSP 47.2. Expression of Heat Shock Protein in the optic nerve heads of primate experimental glaucoma.Expression of HSP was decreased in the optic nerve head in all HSP except for HSP 47. In lamina cribrosa, HSP 47 and 70 showed enhanced expression by immunohistochemistry. In situ hybridization also demonstrated increased expression of HSP 70 and 47 at lamina cribrosa. Lamina cribrosa, contrast to prelamina and retro laminal portion, showed increased expression of HSP may indicate important role for the remodeling of this specific area, which is believed to play an important role for glaucomatous neuropathy.As a summary, HSP may play an important role for the pathogenesis of glaucomatous neuropathy. Expression of HSP was different between retina and optic nerve head in glaucomatous eyes.Further study should be warranted to clear the role of HSP for future possibility whether controlling the HSP expression become neuroprotective regimen for glaucomatous neuropathy.

1. 热休克蛋白在灵长类青光眼视网膜中的表达采用氩激光反复照射腔角制备实验性青光眼模型。然后监测眼压和视神经头。对热休克蛋白抗体进行免疫组化。与正常对照组相比，青光眼视网膜中神经保护性HSP（90,60,27）表达上调。青光眼视网膜中hsp70也有中度升高。与细胞外物质重塑相关的hsp47在青光眼视网膜中的表达无明显变化。这种免疫反应性通过蔡司DAB分析系统进行量化，除HSP 47.2外，所有HSP分子都发生了显著变化。热休克蛋白在灵长类实验性青光眼视神经头中的表达。除hsp47外，其余HSP在视神经头的表达均降低。免疫组化结果显示，hsp47和hsp70在筛板中表达增强。原位杂交也显示在筛板处hsp70和47的表达增加。与椎板前和椎板后部分相比，薄层中HSP的表达增加可能在该特定区域的重塑中起重要作用，被认为在青光眼神经病变中起重要作用。综上所述，热休克蛋白可能在青光眼神经病变的发病机制中起重要作用。青光眼视网膜和视神经头之间HSP的表达存在差异。控制HSP表达是否成为青光眼神经病变的神经保护方案，尚需进一步研究以明确HSP的作用。

项目成果

Miyako Sakai et al.: "Immunolocalization of Heat Shock Proteins in the Retina of Normal Monkey Eyes and Mnkey Eyes with laser-induced Glaucoma"Japan Journal of Ophthalmology. 47. 42-52 (2003)

Miyako Sakai 等人：“正常猴眼和激光诱导青光眼猴眼视网膜中热休克蛋白的免疫定位”日本眼科杂志。