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Role of sugar residues of enamel proteins : Histochemical and Biochemical stud

牙釉质蛋白糖残基的作用：组织化学和生物化学研究

基本信息

批准号：
04671098
负责人：
MATSUO Saburou
金额：
$ 1.34万
依托单位：
Osaka Prefecture University
依托单位国家：
日本
项目类别：
Grant-in-Aid for General Scientific Research (C)
财政年份：
1992
资助国家：
日本
起止时间：
1992 至 1993
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-04671098/
关键词：
Golgi apparatus CoA Pase CMPase NaF Lectin Amelogenin antibody Ameloblast CMP ase NaF エナメル質石灰化糖鎖 golgi装置

项目摘要

To define the role of the sugar residues of enamel proteins in the formation of the tootn enamel, following two experiments were carried out. 1) Involvement of trams-Golgi network (TGN) and the Golgi stack in organizing the Golgi apparatus in ameloblasts of developing molar tooth germs of rat was examined by distribution patterns of two enzymes, CoA pase and CMPase, specific markers for TGN.2) To clarify the influence of fluoride on secretory pathway, changes of lectin staining patterns and amelogenin distribution patterns in the secretory ameloblast were examined in the incisor tooth germs of the rats taken the subcutaneous treatments of sodium fluoride. 1) In the presecretory ameloblast, the saccules with enzymes marked for TGN were observed in the cytoplasm lateral to the nucleus. These saccules were adjacent to the cluster of small vesicles and/or the Golgi stack of different sizes. Upon cell differentiation, Golgi stacks were seen in line along the long axis of the cell, and the f … More ile of the stacks in the cytoplasm lateral to the nucleus were formed. The positive saccules was seen in parallel line equal to the length of the Golgi stacks. In organizing the Golgi apparatus, the development process of the TGN and the Golgi stack appeared to be different. The TGN may participate in geographical determination for the newly forming Golgi stack. 2) The accumulations of small vesicles and abnormal large granules were induced the secretory ameloblast with fluoride treatment. Some large granules contained several small vesicles. Lactin staining patterns were different among the granules. The number of the gold particles detecting amelogenins also varied among the large granules, according to their electron density. The large granules appear to be formed on various steps of the synthesis pathway of the enamel matrix.Moreover, these findings indicate that the fluoride disturb the vesicular transport of the synthesis pathway in the secretory ameloblast, and that the fluoride may interfere both the sorting and fusion on vesicular transport. Less

为了确定牙釉质蛋白中糖残基在牙釉质形成中的作用，进行了以下两个实验。1)本研究以大鼠磨牙牙胚成釉细胞为研究对象，通过对跨高尔基体网络（transm-Golgi network，TGN）和高尔基体堆叠（Golgi stack）的特异性标记物--辅酶A酶（CoA pase）和羧甲基淀粉酶（CMP ase）的分布模式研究了TGN和Golgi stack在成釉细胞高尔基体形成中的作用。结果表明，在大鼠切牙牙胚中，成釉细胞分泌的成釉细胞中的凝集素染色和釉原蛋白分布均发生了变化，氟化钠的治疗1)在分泌前的成釉细胞中，在细胞核外侧的细胞质中观察到带有标记TGN的酶的囊泡。这些囊泡与不同大小的小泡簇和/或高尔基体堆叠相邻。细胞分化后，可以看到高尔基体堆叠沿着细胞的长轴排列，并且f ...更多信息在细胞核外侧的细胞质中形成了一系列的堆叠。阳性囊泡呈平行线排列，与高尔基体的长度相等。在高尔基体的组织中，TGN和高尔基体堆栈的发育过程似乎是不同的。TGN可能参与新形成的高尔基体的地理决定。2)氟诱导分泌型成釉细胞内小泡和异常大颗粒的堆积。一些大颗粒含有几个小泡。颗粒之间的乳糖蛋白染色模式不同。检测釉原蛋白的金颗粒的数量也根据它们的电子密度在大颗粒之间变化。这些结果表明，氟化物干扰了成釉细胞合成途径中的囊泡转运，并可能干扰了囊泡转运的分选和融合。少