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The role of oligosaccharide chains in the formation and the calcification of the enamel matrix

寡糖链在牙釉质基质的形成和钙化中的作用

基本信息

批准号：
02670804
负责人：
MATSUO Saburou
金额：
$ 1.34万
依托单位：
Osaka University
依托单位国家：
日本
项目类别：
Grant-in-Aid for General Scientific Research (C)
财政年份：
1990
资助国家：
日本
起止时间：
1990 至 1991
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-02670804/
关键词：
Enamel Matrix calcification phosphatase Osmium Staining Golgi Apparatus Oligasaccharide Chain lectin

项目摘要

We performed two investigations during the term of this project.1. The cytochemical changes of the Golgi stacks occurring concomitantly with cell differentiation were examined in ameloblasts of developing rat molar tooth germs using osmium impregnation and cytochemistry with NADPase, TPPase, and ACPase. NADPase, TPPase, and ACPase activities were already present in the Golgi stacks of the inner enamel epithelial cells and the presecretory ameloblast : NADPase activity existed in the medial Golgi cisternae, TPPase activity in the trans Golgi cisternae, and ACPase activity in almost all cisternae and strongly in the trans-most cisterna of the Golgi stack. At these stage, however, osmium deposits after impregnation were not observed in the cisterna of the Golgi stacks. Osmiophilic cisternae in the Golgi stacks were apparent for the first time at the stage when the Golgi apparatus developed and migrated to the region dist4-l to the nucleus with the progression of cell differentiation. Thes … More e findings indicate that the cis subcompartment of the Golgi apparatus was incomplete in the inner enamel epithelial cells and the presecretory ameloblast. It is suggested that the cis subcompartment of the Golgi stack may be completed only in the last stage of the compartmentalized Golgi organization during differentiation of the ameloblast.2. The functional modulation of the Golgi stacks during cell differentiation was examined in the ameloblasts of developing rat molar tooth germs, using HRPlabeled lectins ; Con A GS-I, SBA, UEA-I, WGA and PNA. The Golgi stacks of the inner enamel epithelial cells and presecretory ameloblasts were stained with five lectins except for PNA. In some cases, the reaction products for the lectins were observed in most cisternae of the Golgi stacks. In the secretory ameloblasts, however, the reaction products were found in definite cisternae of the Golgi stack, so that discrete staining pattern of the Golgi stack was found for each lectin. These findings indicate that various glycosyltransferase may coexist in most cisternae of the Golgi stack of the inner enamel epithelial cells and presecretory ameloblasts. Moreover, the staining patterns of the lectins in secretory ameloblasts suggest that these cells secrete proteins with N-linked and 0-linked oligosaccharide chains. Less

在这个项目期间，我们进行了两次调查。采用锇浸染和NADPase、TPPase和ACPase的细胞化学方法，研究了大鼠磨牙胚成釉细胞高尔基体在细胞分化过程中的细胞化学变化。NADPase、TPPase和ACPase活性已经存在于内牙釉质上皮细胞和分泌前成釉细胞的高尔基池中：NADPase活性存在于内侧高尔基池中，TPPase活性存在于反式高尔基池中，ACPase活性几乎存在于所有的高尔基池中，并在高尔基池的反式池中表现强烈。然而，在这一阶段，在高尔基堆的贮水池中未观察到浸渍后的锇沉积。在高尔基体发育阶段，随着细胞分化的进行，高尔基体向细胞核的dist4- 1区迁移，高尔基体中的嗜锇池首次出现。进一步的结果表明，高尔基体顺式亚室在内釉上皮细胞和分泌前成釉细胞中是不完整的。这表明，在成釉细胞分化过程中，高尔基堆叠的顺式亚室可能仅在分区高尔基组织的最后阶段完成。利用hrp标记凝集素，研究了大鼠磨牙胚成釉细胞高尔基体在细胞分化过程中的功能调节；Con GS-I， SBA, UEA-I， WGA和PNA。除PNA外，用5种凝集素对内牙釉质上皮细胞和分泌前成釉细胞的高尔基堆积进行染色。在某些情况下，凝集素的反应产物在大多数高尔基堆的池中被观察到。然而，在分泌性成釉细胞中，反应产物是在高尔基堆的特定池中发现的，因此每种凝集素都有离散的高尔基堆染色模式。这些结果表明，多种糖基转移酶可能共存于内釉上皮细胞和分泌前成釉细胞的高尔基堆池中。此外，分泌性成釉细胞中凝集素的染色模式表明，这些细胞分泌具有n -连接和0-连接低聚糖链的蛋白质。少