Function and structure of Streptococus sobrinus glucosyltransferase responsible for water-soluble glucan synthesis

负责水溶性葡聚糖合成的链球菌葡萄糖基转移酶的功能和结构

基本信息

  • 批准号:
    04671100
  • 负责人:
  • 金额:
    $ 1.28万
  • 依托单位:
  • 依托单位国家:
    日本
  • 项目类别:
    Grant-in-Aid for General Scientific Research (C)
  • 财政年份:
    1992
  • 资助国家:
    日本
  • 起止时间:
    1992 至 1993
  • 项目状态:
    已结题

项目摘要

Streptococcus cricetus has four kinds of glucosyltransferase (GTFs), i.e.GTF-I,GTF-S,GTF-SI and GTF-T.These enzymes co-operatively synthesize adhesive water-insoluble glucan from sucrose, which contributes firm adherence of growing organisms to smooth dental surfaces.In this study, the genes encoding glucosyltransferase reponsible for water-soluble glucan synthesis (GTF-S and GTF-T) of S.cricetus HS-6 were cloned. THe sequence of GTF-S genes from S.cricetus and S.downei were compered and show a high degree of homology. There is a 73.9% homology of nucleotides in the genes, and 77.4% homology of amino acids in the deduced protein sequences. In comparison of GTF-T genes with S.cricetus and S.sobrinus, there is 79.7% homology in the genes, and 83.5% homology in the deduced protein sequences.The availability of recombinant E.coli carrying the these genes will greatly facilitate purification of the enzymes for biochemical studies.
链球菌具有4种葡糖转移酶,即GTF-I、GTF-S、GTF-SI和GTF-T,这些酶协同蔗糖合成粘附性的水不溶性葡聚糖,使生长的生物体牢固地附着在光滑的牙面上。比较了中国链球菌和唐氏链球菌的gtf-S基因序列,显示出高度的同源性。基因核苷酸同源性为73.9%,氨基酸同源性为77.4%。GTF-T基因与链球菌和远缘链霉菌的同源性为79.7%,推导的蛋白质序列同源性为83.5%,为进一步纯化生化研究用的酶制剂奠定了基础。

项目成果

期刊论文数量(2)
专著数量(0)
科研奖励数量(0)
会议论文数量(0)
专利数量(0)
陳 慧貞: "Streptococcus cricetus GTF-S遺伝子のクローニング" 日本細菌学雑誌. 48. 138 (1993)
陈慧珍:“仓鼠链球菌 GTF-S 基因的克隆”日本细菌学杂志 48. 138 (1993)。
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FUKUI Kazuhiro其他文献

Statistical Analysis of Phase-Only Correlation Functions under the Phase Fluctuation of Signals due to Additive Gaussian Noise
加性高斯噪声引起的信号相位波动下纯相位相关函数的统计分析

FUKUI Kazuhiro的其他文献

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{{ truncateString('FUKUI Kazuhiro', 18)}}的其他基金

3D protein analysis based on the multiple view images
基于多视图图像的 3D 蛋白质分析
  • 批准号:
    25540062
  • 财政年份:
    2013
  • 资助金额:
    $ 1.28万
  • 项目类别:
    Grant-in-Aid for Challenging Exploratory Research
Study of stereo vision based on simulation driven pattern recognition
基于仿真驱动模式识别的立体视觉研究
  • 批准号:
    23650081
  • 财政年份:
    2011
  • 资助金额:
    $ 1.28万
  • 项目类别:
    Grant-in-Aid for Challenging Exploratory Research
A practical system of learning finger alphabets with visual feedback using a shape correlation map
使用形状相关图通过视觉反馈学习手指字母的实用系统
  • 批准号:
    22300195
  • 财政年份:
    2010
  • 资助金额:
    $ 1.28万
  • 项目类别:
    Grant-in-Aid for Scientific Research (B)
Three-dimensional structural analysis and development of new inhibitors of glucan synthetase produced by cariogenic oral streptococci
致龋口腔链球菌新型葡聚糖合成酶抑制剂的三维结构分析及开发
  • 批准号:
    13557161
  • 财政年份:
    2001
  • 资助金额:
    $ 1.28万
  • 项目类别:
    Grant-in-Aid for Scientific Research (B)
Analysis of domain interaction in insoluble glucan synthetase of oral streptococci
口腔链球菌不溶性葡聚糖合成酶结构域相互作用分析
  • 批准号:
    13671904
  • 财政年份:
    2001
  • 资助金额:
    $ 1.28万
  • 项目类别:
    Grant-in-Aid for Scientific Research (C)
Molecular biological analysis of oral bacterial population dynamics
口腔细菌种群动态的分子生物学分析
  • 批准号:
    10671699
  • 财政年份:
    1998
  • 资助金额:
    $ 1.28万
  • 项目类别:
    Grant-in-Aid for Scientific Research (C)
Study on the structure of the group of homeomorphisms
同胚群的结构研究
  • 批准号:
    10640096
  • 财政年份:
    1998
  • 资助金额:
    $ 1.28万
  • 项目类别:
    Grant-in-Aid for Scientific Research (C)
Ecological analysis of Helicobacter pylori in oral cavities
口腔幽门螺杆菌的生态学分析
  • 批准号:
    08672069
  • 财政年份:
    1996
  • 资助金额:
    $ 1.28万
  • 项目类别:
    Grant-in-Aid for Scientific Research (C)
Function and structure of glucosyltransferase of Streptococcus sobrinus
远缘链球菌葡萄糖基转移酶的功能和结构
  • 批准号:
    06671811
  • 财政年份:
    1994
  • 资助金额:
    $ 1.28万
  • 项目类别:
    Grant-in-Aid for General Scientific Research (C)
Cloning of the gene encoding glucosyltransferase from Streptococcus sobrinus
远缘链球菌葡萄糖基转移酶编码基因的克隆
  • 批准号:
    63570871
  • 财政年份:
    1988
  • 资助金额:
    $ 1.28万
  • 项目类别:
    Grant-in-Aid for General Scientific Research (C)

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糖基神经酰胺在心力衰竭和恢复中的作用
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