Functional and morphological analysis of the main excretory duct IV.

主排泄管的功能和形态分析 IV．

• 批准号: 04671115
• 负责人: SATO Atsuko
• 金额: $ 1.34万
• 依托单位: Fukuoka Dental College
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for General Scientific Research (C)
• 财政年份: 1992
• 资助国家: 日本
• 起止时间: 1992 至 1994
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-04671115/
• 关键词: Tuft cell; Main excretory duct; Submandibular gland; Glycoconjugate cytochemistry; Lectins; ruthenium red; Postnatal development; Male rat; ルテニユ-ム レッド; Submandibular gland; Main excretory duct; Tuft cells; postnatal development; Rat(Wister st

The tuft cells found in the main excretory duct epithelium of the submandibular gland are also present in the hollow organs of endodermal orgin such as the respiratory systems and the alimentary systems. Their common chracteristics are the finger-like long microvilli, microtubules and microfilaments running parallel to the cell axis, and a lot of vesicles and tubules in the apical cytoplasm. However, their functions are still unknown. The following experiments were done to establish their functions1)Vesicles and tubules contained the filamentous substance. Glycoconjugate cytochemistry were performed to prove the character of the filamentous substance. Vesicles, tubules Golgi elements and plasma membrane of the microvilli stained positive.2)The tuft cells were studied by embedding water soluble resin LRW and postembedded staining with lectin/ colloidal-gold (CG) conjugate-Con-A,PNA,SBA,UEA-1, DBA.Con-A was observed in the vesicles and tubules in the apical cytoplasm.3)The postnatal development of tuft cells at various stages, 0,1,7,14,21,28 and 54 days, was investigated by scanning and transmission electron microscopy. Tuft cells were already recognized at birth. Their number reached approximately 6% of the total epithelial cells. The frequency of tuft cells in the adult stage was about 7%. However, microvilli were smaller than the adult ones. In fine structure, the tuft cells at 28 days after birth reached the same level as that at birth.ConclusionThe tuft cells in the main excretory duct epithelium of the rat submandibular gland seem to engage in secretory activity of glycoconjugates, which may be related to the reception because of long microvilli protruding into the lumen.

在下颌下腺主要排泄管上皮中发现的簇状细胞也存在于内胚层起源的中空器官如呼吸系统和消化系统中。它们的共同特征是指状长微绒毛、平行于细胞轴的微管和微丝，顶端细胞质中含有大量的小泡和微管。然而，它们的功能仍然未知。1）囊泡和小管中含有丝状物质。糖缀合物细胞化学证实了丝状物质的特征。2）用LRW水溶性树脂包埋法和凝集素/胶体金（CG）偶联物-Con-A、PNA、SBA、UEA-1、DBA对毛簇细胞进行染色，发现Con-A存在于毛簇细胞顶端细胞质的囊泡和小管中; 3）对毛簇细胞在生后不同时期（0、1、7、14、21、22、24、26、28、29、28和54天，通过扫描和透射电子显微镜进行了研究。毛簇细胞在出生时就已经被识别出来。它们的数量达到总上皮细胞的约6%。成体期丛状细胞的出现率约为7%。但微绒毛比成虫小。结论大鼠下颌下腺主排泄管上皮中的簇状细胞具有糖复合物的分泌活性，这可能与其具有长的微绒毛伸入腺腔而接受糖复合物有关。

项目成果

Sato, A.: "Fine structure of the tuft cells in the main excretory duct of the rat submandibular gland." Cell Tissue Res. (in press). (1995)

Sato, A.：“大鼠颌下腺主要排泄管簇细胞的精细结构。”

Sato, A.: "Postnatal development of the tuft cells of the rat submandibular gland." Anatomical Record. (in press). (1995)

Sato, A.：“大鼠颌下腺簇细胞的产后发育。”

Sato,A.: "Fine structure of The tuft cells inthe main excretory duct of the rat submandibular gland." Cell Tissue Res. (in press). (1995)

Sato,A.：“大鼠颌下腺主要排泄管簇细胞的精细结构。”

Atsuko SATO: "Fine structure and subepithelial capillaries of the main excretory duct epithelium of the submandibular gland of the japanese monkey,Macaca fuscara." Anatomical Record. (1993)

Atsuko SATO：“日本猴颌下腺主排泄管上皮的精细结构和上皮下毛细血管。”

Sato,A.: "Postnatal development of the tuft cells of the rat submandibular gland." Anatomical Record. (in press). (1995)

Sato,A.：“大鼠颌下腺簇细胞的产后发育。”