Functional and morphological analysis orme main excretory auct epithelium of the salivary gland. VI. Secretory ability of the tuft cell

1.微绒毛表面和丛生细胞的管泡状系统具有大豆凝集素活性，表明它们之间存在一定的关系。三维重建的绒毛细胞显示了顶端-绒毛细胞之间的关系，以及由复杂而连贯的小泡和细小的小管网络分别组成的小管泡状系统，提示膜的存量有利于表面积的快速变化。用光镜和电子显微镜放射自显影技术研究了主排卵管中簇状细胞分泌糖蛋白的能力。大鼠静脉注射糖蛋白前体~3H-半乳糖。分别于注射~3H-半乳糖后5min、20min、2小时、6h、24小时处死动物。结果表明，簇状细胞很容易将~3H-半乳糖掺入膜糖蛋白中，而膜糖蛋白并入质膜而不能将糖蛋白分泌到唾液中。

1. The surface of microvilli and the tubulovesicular system of the tuft cell exhibited the SBA (Soybean agglutinin) reactivity, suggesting a elationship between them.2. 3-D reconstruction of the tuft cell reveald the hypolemmal terminal-tuft cell relationship and that tubulovesicular system composed of the complex and coherent vesicles and small network of tubules separated respectively, suggesting the stock of membrane for rapid change of the surface area.3. The ability to secrete the glycoprotein by the tuft cells in the main exc etory duct was investigated with light and electron microscope radioautographic study. The glycoprotein precursor 3H-galactose was injected intravenously into rats. At 5min, 20min, 2h,6h, 24h after injection of ^3H-galactose, animals were sacrificed. The results indicated that the tuft cells readily incorporate ^3H-galactose into the membrane glycoproteins, which are incorporated into the Plasma membrane and that have no ability to secrete theglycoprotein into the saliva.