Functional and morphological analysis orme main excretory auct epithelium of the salivary gland. VI. Secretory ability of the tuft cell

功能和形态分析orme主要排泄唾液腺上皮。

• 批准号: 11671833
• 负责人: SATO Atsuko
• 金额: $ 2.24万
• 依托单位: Fukuoka Dental College
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 1999
• 资助国家: 日本
• 起止时间: 1999 至 2001
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-11671833/
• 关键词: tuft cell; main excretory duct; submandibu ar gland; lectin cytochemistry; 3-D reconstruction; radioautography; ^3H-galactose; rat; light cell type I; light cell type II; オートラジオグラフィー; レクチン

1. The surface of microvilli and the tubulovesicular system of the tuft cell exhibited the SBA (Soybean agglutinin) reactivity, suggesting a elationship between them.2. 3-D reconstruction of the tuft cell reveald the hypolemmal terminal-tuft cell relationship and that tubulovesicular system composed of the complex and coherent vesicles and small network of tubules separated respectively, suggesting the stock of membrane for rapid change of the surface area.3. The ability to secrete the glycoprotein by the tuft cells in the main exc etory duct was investigated with light and electron microscope radioautographic study. The glycoprotein precursor 3H-galactose was injected intravenously into rats. At 5min, 20min, 2h,6h, 24h after injection of ^3H-galactose, animals were sacrificed. The results indicated that the tuft cells readily incorporate ^3H-galactose into the membrane glycoproteins, which are incorporated into the Plasma membrane and that have no ability to secrete theglycoprotein into the saliva.

1.毛簇细胞的微绒毛表面和管泡系统均表现出大豆凝集素（SBA）反应性，表明二者之间存在一定的联系.簇状细胞的三维重建显示了下膜终末-簇状细胞的关系，由复杂而连贯的小泡和小的小管网络组成的管泡系统分别分离，提示了表面积快速变化的膜储备.用光镜和电镜放射自显影技术研究了主外分泌管簇状细胞分泌糖蛋白的能力。给大鼠静脉注射糖蛋白前体3 H-半乳糖。分别于注射^3H-半乳糖后5 min、20 min、2 h、6 h、24 h处死动物。结果表明，毛簇细胞易于将^3H-半乳糖掺入膜糖蛋白中，而膜糖蛋白则掺入质膜，但不能将糖蛋白分泌到唾液中。

项目成果

Three-demensional structure of apical vesicles of tuft cell of the main excretory duct in the rat submandibulargland.

大鼠颌下腺主排泄管簇细胞顶端小泡的三维结构。

• 发表时间: 2003
• 期刊: Eur J Morphol (in press)
• 作者: Sato A.

Sato,A.,et al.: "Tuft cells in the main excretory duct of the rat submandibular gland."Eur.J.Morph.. 4(in press). (2000)

Sato, A., et al.：“大鼠颌下腺主要排泄管中的簇细胞。”Eur.J.Morph.. 4（印刷中）。

Three-demensional stucture of apical vesicles of tuft cell of the main excretory duct in the rat submandibular gland.

大鼠颌下腺主排泄管簇细胞顶泡三维结构。

• 发表时间: 2003
• 期刊: Eur J Morphol (in press)
• 作者: Sato A.;Hisanaga Y.;Inoue Y.;Nagato T.;Toh H.
• 通讯作者: Toh H.

Tuft cell in the main excretory dt ct of the rat submandibular gland.

大鼠颌下腺主要排泄性dt ct中的簇细胞。

• 发表时间: 2000
• 期刊: Eur J Morphol 38(4)
• 作者: Sato A.;Suganuma T.;Ide S.;Kavano J.;Nagato T.
• 通讯作者: Nagato T.

Tuft cell in the main excretory duct of the rat submandibular gland.

大鼠颌下腺主排泄管中的簇细胞。

• 发表时间: 2000
• 期刊: Eur J Morphol 38
• 作者: Sato A.;Hisanaga Y.;Inoue Y.;Nagato T.;Toh H.;Sato A.
• 通讯作者: Sato A.