Genes associated with brain-tropic human melanoma cells and their function

转移形成是大多数癌症死亡的原因。脑转移，最常见的黑色素瘤与肺癌和乳腺癌，导致高发病率和死亡率。我们使用生物发光成像(BLI)技术对许多人类癌细胞株的分析表明，癌细胞类型依赖于NOD/SCID小鼠特定器官的转移。在此，我们建立了亲脑黑色素瘤细胞系SK-MEL-28-Luc-BR2和Mewo-Luc-BR2。接种小鼠，测定注射后黑色素瘤细胞在脑内蓄积的动力学。注射后0.5-5小时，嗜脑黑色素瘤细胞和低转移黑色素瘤细胞(SK-MEL-2-Luc)在脑内均有类似程度的存在，而嗜脑黑色素瘤细胞在晚期(15-20天)肿瘤来源的光子显著增加。此外，全面的转录组分析显示，在嗜脑黑色素瘤和乳腺癌(MDA-MB-231-Luc-BRL)细胞中，转化生长因子-β受体II的表达显着增加(3-6倍)。BLI介导的体内成像将有助于识别潜在的癌症转移分子标志物。

Metastasis formation is responsible for most cancer deaths. Brain metastases, most often with melanomas as with lung and breast carcinomas, induce a high morbidity and mortality. Our analysis of many human cancer cell lines using bio-luminescent imaging (BLI) technology, has demonstrated cancer cell-type dependent metastasis to specific organs of NOD/SCID mice. Herein we established brain-tropic melanoma cell lines (SK-MEL-28-luc-br2 and Mewo-luc-br2) after 3 cycles of sequential intra-cardiac (i.e.) inoculation into mice, and determined the kinetics of brain accumulation of melanoma cells after i.e. injection. While both the brain-tropic and the low-metastatic melanoma (SK-MEL-2-luc) cells were present to similar degrees in the brain 0.5-5 hrs after injection, brain-tropic melanoma cells showed a significant increase in tumor-derived photons at the late phase (after 15-20 days). Further a comprehensive transcriptome analysis showed a significant increase (3-6 fold) of TGF-beta receptor II in brain-tropic melanoma and breast carcinoma (MDA-MB-231-luc-brl) cells. The BLI-mediated in vivo imaging should promote identification of potential molecular markers for cancer metastasis.