Genes associated with brain-tropic human melanoma cells and their function

转移形成是大多数癌症死亡的原因。脑转移瘤，最常见的黑色素瘤与肺癌和乳腺癌，诱导高发病率和死亡率。我们使用生物发光成像（BLI）技术对许多人类癌细胞系的分析已经证明了NOD/SCID小鼠的特定器官的癌细胞类型依赖性转移。本文中，我们在3个周期的连续心内（即，接种到小鼠中，并测定即注射后黑素瘤细胞的脑积累的动力学。虽然在注射后0.5-5小时，向脑和低转移性黑素瘤（SK-MEL-2-luc）细胞在脑中以相似的程度存在，但向脑黑素瘤细胞在晚期（15-20天后）显示出肿瘤来源的光子的显著增加。进一步的综合转录组分析显示，在嗜脑性黑色素瘤和乳腺癌（MDA-MB-231-luc-brl）细胞中TGF-β受体II显著增加（3-6倍）。BLI介导的体内成像应促进癌症转移的潜在分子标记物的鉴定。

Metastasis formation is responsible for most cancer deaths. Brain metastases, most often with melanomas as with lung and breast carcinomas, induce a high morbidity and mortality. Our analysis of many human cancer cell lines using bio-luminescent imaging (BLI) technology, has demonstrated cancer cell-type dependent metastasis to specific organs of NOD/SCID mice. Herein we established brain-tropic melanoma cell lines (SK-MEL-28-luc-br2 and Mewo-luc-br2) after 3 cycles of sequential intra-cardiac (i.e.) inoculation into mice, and determined the kinetics of brain accumulation of melanoma cells after i.e. injection. While both the brain-tropic and the low-metastatic melanoma (SK-MEL-2-luc) cells were present to similar degrees in the brain 0.5-5 hrs after injection, brain-tropic melanoma cells showed a significant increase in tumor-derived photons at the late phase (after 15-20 days). Further a comprehensive transcriptome analysis showed a significant increase (3-6 fold) of TGF-beta receptor II in brain-tropic melanoma and breast carcinoma (MDA-MB-231-luc-brl) cells. The BLI-mediated in vivo imaging should promote identification of potential molecular markers for cancer metastasis.