Effects of substance P and phenolic compounds on proliferation of pulpal fibroblasts.

P物质和酚类化合物对牙髓成纤维细胞增殖的影响。

• 批准号: 04671148
• 负责人: OHKUBO Tsuyako
• 金额: $ 1.22万
• 依托单位: Fukuoka Dental College
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for General Scientific Research (C)
• 财政年份: 1992
• 资助国家: 日本
• 起止时间: 1992 至 1993
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-04671148/
• 关键词: Substance P; Eugenol; Guaiacol; Fibroblast; Proliferation; フエノール系薬剤; サブスタンス P; 歯髄線維芽細胞; 細胞増殖

Substance P,a neuropeptide released from sensory nerve endings, acts as a neurotrophic factor for tissue regeneration or structural retention in addition to a chemical mediator and a neurotransmitter. On the other hand, phenolic dental medicaments such as eugenol and guaiacol are used in dental clinics especially for the treatment of dental pulpitis. It was recently reported that these phenolic compounds had a stimulative effect on proliferation of human pulpal fibroblasts. In the present study, to clarify the mechanism of action of phenolic compounds on the processes of tissue repairment in inflammation, proliferation of pulpal fibroblasts by these drugs was investigated in relation to the neurotrophic action of substance P.Fibroblasts were isolated from rat inferior incisor pulp by treatment with 0.2% collagenase. Cells were cultured in Dulbecco's modified Eagle's medium with 10% fetal calf serum. Cell proliferation was measured by ^3H-thymidine uptake following 24 h exposure to agents. Substance P(10^<-9>-10^<-6> g/ml) significantly increased cell proliferation. Eugenol(10^<-8>-10^<-6> g/ml) and guaiacol(10^<-8>-10^<-6> g/ml) also increased cell proliferation. 0.005% eugenol and guaiacol caused a significant increase in substance P release from dental pulp tissue in vitro. These results suggest that the action of eugenol and guaiacol may be due in part to the ability to release substance P and the subsequent stimulative effect by substance P,besides their direct effects on cell proliferation.

PESTANCE P是一种从感觉神经端释放的神经肽，除了化学介质和神经递质外，还充当组织再生或结构保留的神经营养因子。另一方面，在牙科诊所中使用了酚类牙科药物，例如尤金尔和瓜艾醇，特别是用于治疗牙髓炎。最近有报道称，这些酚类化合物对人牙髓成纤维细胞的增殖具有刺激作用。在本研究中，为了阐明酚类化合物在炎症中组织修复过程的作用机理，研究了这些药物对牙髓成纤维细胞的增殖，从而通过使用0.2％colagenenspase将大鼠牙切除叶乳清蛋白酶从大鼠下切除叶骨中分离出与物质性伪细胞的神经营养作用有关。将细胞培养在Dulbecco的改良鹰培养基中，胎牛血清含量为10％。在24小时暴露于药物后，通过 ^3H-胸腺苷的摄取来测量细胞增殖。物质P（10^<-9> -10^<-6> g/ml）显着增加了细胞增殖。 Eugenol（10^<-8> -10^<-6> g/ml）和瓜艾亚醇（10^<-8> -10^<-6> g/ml）也增加了细胞增殖。 0.005％的Eugenol和Guiacol在体外从牙髓组织中释放P的物质显着增加。这些结果表明，除了对细胞增殖的直接影响外，尤金尔和瓜埃亚尔的作用可能部分是由于释放物质P的能力以及随后对PESTS P的刺激作用。