Molecular cloning of cDNA coding for the salivary proline-rich peptide P-B

以人颌下腺cDNA为模板，以d（脱氧）T_为双链引物，用聚合酶链反应（PCR）扩增了人唾液富含脯氨酸肽P-B的cDNA<25>。该cDNA全长0.8kbp，编码22个氨基酸的疏水信号序列和57个氨基酸的P-B全序列。P-B cDNA的核苷酸序列测定表明，P-B是一个成熟的全长蛋白质，而不是一个较大的蛋白质的酶降解产物。编码P-B的基因不是先前表征的富含脯氨酸的蛋白质基因家族的成员。P-B cDNA的核苷酸序列出现在GDBS、DDBJ、EMBL和NCB 1核苷酸序列数据库中，登录号为D29833。

cDNA coding for the human salivary proline-rich peptide P-B was amplified by polymerase chain reaction using human submaxillary gland cDNA as the template and d (deoxy) T_<25> as the primer for both strands. The amplified cDNA of 0.8 kbp encodes a hydrophobic signal sequence of 22 amino acid residues and the complete sequence of P-B (57 amino acids). The determined nucleotide sequence of P-B cDNA indicates that P-B is a mature full-size protein and not the enzymic degradation product of a larger protein. The gene coding for P-B is not a member of the proline-rich protein gene family characterized previously. The nucleotide sequence of P-B cDNA appears in the GDBS,DDBJ,EMBL and NCBl nucleotide sequence databases with the accession number D29833.