Distribution and forms of fluoride in normal fuman plasma

正常福曼血浆中氟化物的分布和形态

• 批准号: 04671271
• 负责人: KOYAMA Masahide
• 金额: $ 1.34万
• 依托单位: Showa University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for General Scientific Research (C)
• 财政年份: 1992
• 资助国家: 日本
• 起止时间: 1992 至 1993
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-04671271/
• 关键词: Gel chromatography; Ionic fluoride; Protein bound fluoride; Organic fluoride compound; Serum; Plasma; Hard tissue; フッ素; 動物実験; 動態; ヒト

The objective of this research was to determine by the gel chromatography method the basal range and the average concentration of fluoride in human plasma. The fluoride levels in the rat organs were measured when the organic fluoride compound (DF) and sodium fluoride were administered by gastric intubation once a day while breeding on a low fluoride diet.The results were as follows :1.In a polot study using market human serum, three types of fluoride (ionic, non-ionic bound and non-ionic low molecular) were measured, the results were similar to those of previous data concerning plasma and serum of animals^<*\)>. Therefore, the gelchromatography method proved to be an effective technique for the study of the three types of fluoride in plasma and serum.2.However, the gel chromatography method was not successful for the study of the plasma and serum taken from volunteers because most of the non-ionic fluoride in the plasma was bound fluoride resulting in a bound fluoride level much higher than the non-ionic fluoride level. These facts suggest that the gel chromatography method must be investigated further with various animal types and cmponents of plasma and serum.3.The organic fluoride compound (DF) dose group did not result in an increase of ionic fluoride in the plasma. However, the sodium fluoride dose group shwed an increase of ionic fluoride in the plasma and the hard tissues is related to the ionic fluoride level in plasma because the ionic fluoride cancentration in hard tissues showed a lebel in plasma because the ionic fluoride concentration in hard tissuses showed a similar tendency in the plasma.4.The low fluoride diet was useful for the study of different trace amounts of fluoride of plasma and other tissues in animal experiments.^<*\)>Koyama M., et al. : A study on fluoride in plasma protein by gel chromatography, J.Dent. Health, 40, 456-457, 1990. (in japanese)

本研究的目的是用凝胶色谱法测定人体血浆中氟的基线范围和平均浓度。在低氟饲养条件下，每天一次灌胃给予有机氟化物（DF）和氟化钠，测定大鼠各器官氟含量，结果如下：1.用市售人血清进行Polot法，测定了离子型、非离子型和非离子型低分子氟，结果与动物血浆和血清的结果相似。因此，凝胶色谱法是研究血浆和血清中三种氟的有效方法。2.然而，凝胶色谱法对志愿者血浆和血清的研究并不成功，因为血浆中的非离子氟大部分是结合氟，导致结合氟水平远高于非离子氟水平。这些事实表明，凝胶色谱法必须进一步研究各种动物类型和血浆和血清成分。3.有机氟化物（DF）剂量组没有导致血浆中离子氟的增加。然而，在这方面，氟化钠剂量组血浆氟离子浓度升高，而硬组织氟离子浓度与血浆氟离子浓度相关，因为血浆氟离子浓度与硬组织氟离子浓度呈相似趋势，而硬组织氟离子浓度与血浆氟离子浓度呈显著负相关。动物实验中的其他组织。<*\）>Koyama M.，等人：A study on fluoride in plasma protein by gel chromatography，J. Dent. Health，40，456-457，1990. (in（日文）

项目成果

古山公英: "有機および無機フッ素投与ラットの組織中フッ素濃度,ジフルニサルとNaF投与" 口腔衛生学会雑誌. 43. 474-475 (1993)

Kimihide Furuyama：“给予有机和无机氟化物、二氟尼柳和 NaF 的大鼠的组织氟化物浓度”口腔健康协会杂志 43. 474-475 (1993)。

KOYAMA.M, TOMITA.M, KANEKO.Y: "Fluoride levels in tissues of rat after administration of organic and inorganic fluoride compounds, Diflunisal and NaF" Journal of Dental Health (in Japanese). 43. 474-475 (1993)

KOYAMA.M、TOMITA.M、KANEKO.Y：“给予有机和无机氟化物、二氟尼柳和氟化钠后大鼠组织中的氟化物水平”《牙科健康杂志》（日语）。

古山,公英: "有機および無機フッ素投与ラットの組織中フッ素濃度,ジフルニサルとNaF投与" 口腔衛生学会雑誌. 43. 474-475 (1993)

Furuyama, Kimihide：“给予有机和无机氟化物、二氟尼柳和 NaF 的大鼠的组织氟浓度”口腔健康协会杂志 43. 474-475 (1993)。