Distribution and forms of fluoride in normal fuman plasma

本研究的目的是用凝胶层析法测定人血浆中氟的基础浓度范围和平均浓度。在低氟饲料饲养的同时，每天一次通过胃管给大鼠灌胃有机氟化合物(DF)和氟化钠。结果如下：1.在Polot研究中，用市售人血清测定了三种类型的氟(离子、非离子结合和非离子低分子)，结果与以往动物血浆和血清的数据相似。因此，凝胶层析法是研究血浆和血清中三种氟化物的一种有效方法。然而，对于志愿者的血浆和血清，凝胶层析法并不成功，因为血浆中的大多数非离子氟是结合氟，导致结合氟水平远远高于非离子氟水平。这些事实表明，凝胶层析法必须进一步研究不同动物类型和不同成分的血浆和血清。3.有机氟化合物(DF)剂量组没有引起血浆中离子氟的增加。然而，氟化钠剂量组血浆和硬组织中离子氟浓度的增加与血浆中离子氟水平有关，因为硬组织中离子氟浓度在血浆中表现出明显的变化趋势，而硬组织中离子氟浓度与血浆中离子氟浓度的变化趋势相似。4.低氟饮食可用于动物实验中血浆和其他组织中不同微量氟的研究。用凝胶层析法研究血浆蛋白中的氟化物。健康，40,456-457,1990。(日语)

The objective of this research was to determine by the gel chromatography method the basal range and the average concentration of fluoride in human plasma. The fluoride levels in the rat organs were measured when the organic fluoride compound (DF) and sodium fluoride were administered by gastric intubation once a day while breeding on a low fluoride diet.The results were as follows :1.In a polot study using market human serum, three types of fluoride (ionic, non-ionic bound and non-ionic low molecular) were measured, the results were similar to those of previous data concerning plasma and serum of animals^<*\)>. Therefore, the gelchromatography method proved to be an effective technique for the study of the three types of fluoride in plasma and serum.2.However, the gel chromatography method was not successful for the study of the plasma and serum taken from volunteers because most of the non-ionic fluoride in the plasma was bound fluoride resulting in a bound fluoride level much higher than the non-ionic fluoride level. These facts suggest that the gel chromatography method must be investigated further with various animal types and cmponents of plasma and serum.3.The organic fluoride compound (DF) dose group did not result in an increase of ionic fluoride in the plasma. However, the sodium fluoride dose group shwed an increase of ionic fluoride in the plasma and the hard tissues is related to the ionic fluoride level in plasma because the ionic fluoride cancentration in hard tissues showed a lebel in plasma because the ionic fluoride concentration in hard tissuses showed a similar tendency in the plasma.4.The low fluoride diet was useful for the study of different trace amounts of fluoride of plasma and other tissues in animal experiments.^<*\)>Koyama M., et al. : A study on fluoride in plasma protein by gel chromatography, J.Dent. Health, 40, 456-457, 1990. (in japanese)