RESEARCH ON SECRETORY MECHANISM OF HYPOTHALAMIC

下丘脑分泌机制的研究

• 批准号: 04671486
• 负责人: BANDO Hiroshi
• 金额: $ 1.34万
• 依托单位: THE UNIVERSITY OF TOKUSHIMA
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for General Scientific Research (C)
• 财政年份: 1992
• 资助国家: 日本
• 起止时间: 1992 至 1993
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-04671486/
• 关键词: Microdialysis; Hypothalamus; Somatostatin; TRH; GHRH; Circadian rhythm; Rat; Microdialysis; Hypothalamus; Somatostatin; Circadian rhythm; Rat; pituitary

We inestigated for these two years secretory mechanism of hypothalamic hormones such as somatostatin (SRIF) and Thyrotropin-Releasing hormone (TRH) using method of brain microdialysis, and we now summarize these results.1) In measuring the extremely small amount of hypothalamic hormones by brain microdialysis method, we clarified the usefulness and adequecy of PC and PS membranes.2) Secretory mechanism of somatostain (SRIF) from the hypothalamus : We investigated the SRIF concentration in extracellular fluid in rat pituitary gland. The results were follows : The concentration of SRIF in extracellular fluid of the rat pituitary was between 35 to 97 pg/ml, and it showed the circadian rhythm with higher concentration in the light phase (35-55 pg/ml) than in the dark phase (60-97 pg/ml). Furthermore, SRIF concentration occasionally increased in 3-6 hours-interval. When we investigated the influences of various anesthetic agents, SRIF concetration increased with urethane anesthesia, and it decreased with pentobarbital anesthesia.3) Secretory mechanism of Thyrotropin-Releasing Hormone (TRH) : We investigated the TRH concentration in the paraventricular nucleus in the hypothalamus of the rat. TRH concentration in the dialysate ranged from 7.6 to 10 pg/ml, and it showed higher tendency in the dark phase (2.74 (〕SY.+-.〔) 1/65 pg/ml) than in the light phase (2.15 (〕SY.+-.〔) 1.46 pg/ml).4) From these results, we clarified the possibility and usefulness of measuring hypothalamic hormones directly and with dinamically by using the method of brain microdialysis.Furthermore, we are at present investigating the secretory mechanism of Gonadotropin-Releasing Hormone (GnRH) and Growth Hormone-Releasing Hormone (GHRH).

我们用脑微透析的方法研究了两年来下丘脑生长抑素（SRIF）和促甲状腺素释放激素（TRH）的分泌机制，现对这些结果进行总结。1)通过脑微透析法测量极少量的下丘脑激素，我们明确了PC膜和PS膜的有效性和充分性。2)下丘脑分泌生长色素（SRIF）的机制：研究大鼠垂体细胞外液中SRIF的浓度。结果表明：大鼠垂体细胞外液中SRIF浓度在35 ~ 97 pg/ml之间，呈昼夜节律性，光照期浓度（35 ~ 55 pg/ml）高于暗期浓度（60 ~ 97 pg/ml）。SRIF浓度在3 ~ 6 h间偶有升高。不同麻醉药物对SRIF浓度的影响，脲烷麻醉时SRIF浓度升高，戊巴比妥麻醉时SRIF浓度降低。3)促甲状腺激素释放激素（TRH）的分泌机制：研究大鼠下丘脑室旁核TRH的浓度。透析液中TRH浓度在7.6 ~ 10 pg/ml之间，在暗相表现出较高的趋势(2.74 （]SY.+-）。[) 1/65 pg/ml)比光相(2.15 (]SY.+-。[) 1.46 pg/ml)。4)从这些结果中，我们明确了用脑微透析法直接和动态测量下丘脑激素的可能性和实用性。此外，我们目前正在研究促性腺激素释放激素（GnRH）和生长激素释放激素（GHRH）的分泌机制。

项目成果

高橋 秀夫、新谷 保実、石川 雅康、板東 浩、斎藤 史郎他: "ラット下垂体におけるソマトスタチン濃度の日内変動-脳内微小透析法いよる検討" 日本内分泌学会雑誌(抄録). 68. 808 (1992)

Hideo Takahashi、Yasumi Shintani、Masayasu Ishikawa、Hiroshi Bando、Shiro Saito 等：“大鼠垂体生长抑素浓度的昼夜变化 - 使用脑内微透析方法的调查”日本内分泌学会杂志（摘要）68. 808。 (1992))

岡内泰弘、高橋秀夫、板東浩、斎藤史郎、他: "ラット視床下部におけるThyrotropin-Releasing Hormone(TRH)の分泌動態-脳内微小透析法による検討" 日内分泌会誌(抄録). 69. 727 (1993)

Yasuhiro Okauchi、Hideo Takahashi、Hiroshi Bando、Shiro Saito 等：“大鼠下丘脑促甲状腺激素释放激素 (TRH) 的分泌动态 - 使用脑内微透析方法的研究”日本内分泌学会杂志（摘要）69. 727。 (1993))

Takahashi H, Sato S: "Analysis of neuropeptides by brain microdialysis" Folia Endocrinol Japonica. 70(in press). (1994)

Takahashi H、Sato S：“通过脑微透析分析神经肽”Folia Endocrinol Japonica。

三ッ井 貴夫、川井 尚臣 板東 浩、斎藤 史郎他: "サーカディアンリズムの障害を伴った無動性無言症" 神経内科. 37. 11-16 (1992)

Takao Mitsui、Naoomi Kawai、Hiroshi Bando、Shiro Saito 等人：“与昼夜节律紊乱相关的运动不能性沉默症”《神经病学》37. 11-16 (1992)。

Takahashi H.,Bando H.,Saito S.: "Measurement of somatostatin release in the rat brain by microdialysis" J Neurosci Method. 9 (in press). (1994)

Takahashi H.、Bando H.、Saito S.：“通过微透析测量大鼠大脑中生长抑素的释放”J Neurosci Method。