Enzymochemical Study on growth of Psychrotrophs during Food Preservation in Refrigirator

冰箱食品保藏过程中耐冷菌生长的酶化学研究

• 批准号: 04680070
• 负责人: YOKOIGAWA Kumio
• 金额: $ 1.34万
• 依托单位: Nara Women's University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for General Scientific Research (C)
• 财政年份: 1992
• 资助国家: 日本
• 起止时间: 1992 至 1993
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-04680070/
• 关键词: Food preservation; Psychrotroph; Food deterioration; Alanine racemase; 低温菌酵素; アミノ酸異性化酵素

Alanine racemase essential to growth of bacteria was purified from psychrotrophs isolated from foods. The enzymesfrom typical psychrotrophs, Pseudomonas fluorescens, bacillus psychrophilus and Bacillus psychrosaccharolyticus were markedly inactivated when incubated at over 30ﾟC for 1 h, and the bacteria could not growat over 30ﾟC.Thus, the region of growth temperature were consistent with the thermostability of the enzymes. Since the amino acid composition and sequence were similar to those of other alanine racemases from mesophiles and a thermophile, alanine racemase may be suitable for comparative study on thermostability. The enzymes from psychrotrophs were thermolabile, and inactivated by incubation with denaturants andorganic solvents. Especially, 20% ethanol markedly inactivated the thermolabile enzymes even at 0ﾟC.Ethanol was effective as a sterilizer to psychrotrophs.

从食品中分离的耐冷菌中纯化出细菌生长所必需的丙氨酸消旋酶。典型的耐冷菌荧光假单胞菌、嗜冷芽孢杆菌和解糖芽孢杆菌在30℃以上培养1 h后酶明显失活，细菌在30℃以上不能生长，因此，其生长温度范围与酶的热稳定性一致。由于氨基酸组成和序列与其他来自嗜温菌和嗜热菌的丙氨酸消旋酶相似，丙氨酸消旋酶可能适合于热稳定性的比较研究。来自耐冷生物的酶不耐热，并且通过与变性剂和有机溶剂一起孵育而失活。特别是，即使在0°C，20%的乙醇也能显着灭活耐热酶。乙醇对于耐冷生物来说是有效的灭菌剂。

项目成果

Kumio Yokoigawa: "Thermolabile alanine racemase from a psychrotroph,Pseudomonas fluorescens:purification and properties." Biosci.Biotech.Biochem.57. 93-97 (1993)

Kumio Yokoikawa：“来自耐冷菌荧光假单胞菌的耐热丙氨酸消旋酶：纯化和特性。”

Kumio Yokoigawa: "Thermolabile Alanine Racemase from a Psychrotroph,Purification and Properties" Biosci.Biotech.Biochem.57. 93-97 (1993)

Kumio Yokoikawa：“来自耐冷菌的耐热丙氨酸消旋酶、纯化和特性”Biosci.Biotech.Biochem.57。

Young-Hee Lim: "A new amino acid racemase with threonine alpha-epimerase activity from Pseudomonas putida : purification and characterization" J.Bacteriology. 175. 4213-4217 (1993)

Young-Hee Lim：“来自恶臭假单胞菌的具有苏氨酸 α-差向异构酶活性的新型氨基酸消旋酶：纯化和表征”J.Bacteriology。

林英姫: "A new amino acid racemase with threonine α-epimerase activity from Pseudomonas putida:purification and characterization" J.Bacteriology. 175. 4213-4217 (1993)

Hideki Hayashi：“来自恶臭假单胞菌的具有苏氨酸 α-差向异构酶活性的新氨基酸消旋酶：纯化和表征”J.Bacteriology。175. 4213-4217 (1993)

Young-Hee Lim: "A new amino acid racemase with threonine α-epimerase activity from Pseudomonas putida:purification and characterization." J.Bacteriology. 175. 4213-4217 (1993)

Young-Hee Lim：“来自恶臭假单胞菌的具有苏氨酸 α-差向异构酶活性的新型氨基酸消旋酶：纯化和表征。J.Bacteriology”175。4213-4217（1993）