The regulatory mechanism mechanism of cellular activities by extracllular matrix

细胞与细胞外基质的粘附对于大多数动物细胞发挥细胞活动如生长、运动以及纤维连接蛋白和Alminin等是非常重要的。在本研究中，我们研究了细胞对这些蛋白质的附着与细胞活性之间的关系。1.在妊娠早期的人胎盘中，滋养细胞基底侧表达VLA 5纤连蛋白受体。在成熟的胎盘中，合体滋养细胞基底侧表达VLA 6层粘连蛋白受体。这些结果表明，细胞利用特异性受体进行附着，与它们附着的细胞外基质成分相对应。2.在接下来的研究中。我们检测了两种具有不同转移潜能的小鼠肺癌细胞系中层粘连蛋白和67 K层粘连蛋白受体的表达。结果表明，在高转移细胞系中，层粘连蛋白mRNA水平较低，但在高转移细胞系中，层粘连蛋白mRNA水平与高转移细胞系相似。这些发现表明，未被占据的层粘连蛋白受体可能有助于转移性质的差异。3.我们还发现，（-）-表儿茶素和（-）-表没食子儿茶素抑制3LL细胞与纤维连接蛋白的粘附。结果可以解释以前的发现，（-）-表没食子儿茶素抑制这些细胞在体内的肺转移。因此，本工作证明了细胞附着对于各种细胞活动的重要性。

The cell attachment to the extracellular matrixt is very important for most animal cells to exert cell's activities such as growth, locomotion and as fibronectin and alminin are now known. In the present study, we examined the relationship between the cell attachment to these proteins and cellular activities. 1. In the human placenta at early gestation, cytorophoblasts were found to express VLA5 fibronectin receptor on the basal sideIn the mature placenta, syncytiotrophoblasts expressed VLA6 laminin receptor on the basal side. These results indicate that cells make use of specific receptor for their attachment in correspondence to the extracellular matrix component to which they attach. 2. In the next study. we examined the expression of laminin and 67K laminin receptor in two cell lines with different metastatic potentials of murine lung carcinoma. It was demonstrated that the laminin mRNA level was lower in the high metastatic cell lines were at the similar level. These findings suggest that unoccupied laminin receptor may contribute to the difference in metastativ property. 3. We also found that the 3LL cell attachment to fibronectin was inhibited by (-) -epicatechin and (-)-epigallocatechin. The results can explain the previous finding that (-)-epigallocatechin inhibited the lung metastasis of these cells in vivo. Thus, the present work demonstrated the importance of the cell attachment for various cellular activities.